Hybrid photoacoustic and electrophysiological recording of neurovascular communications in freely-moving rats

Xi, Lei; Jin, Tian; Zhou, Junli; Carney, Paul R.; Jiang, Hubei

doi:10.1016/j.neuroimage.2017.08.037

Cited by 18 publications

(11 citation statements)

References 56 publications

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“…Red blood cells conjugated with hemoglobin are perfect photoacoustic absorbers and supply sufficient image contrast. Several high‐speed ORPAMs show the encouraging studies of neurovascular coupling in anesthetized and restricted animal . However, the inconvenience of the systems limits the studies to certain areas in the brain.…”

Section: Resultsmentioning

confidence: 99%

“…It has been successfully demonstrated in both clinical and fundamental studies including early‐stage cancer diagnosis, arthritis detection, drug/nanomaterial evaluation and brain research . Photoacoustic microscopy, especially optical resolution photoacoustic microscopy (ORPAM), has been extensively applied in studying cortical hemodynamic in animals due to the excellent sensitivity to endogenous hemoglobin inside blood vessels, comparable penetration depth with 2/multiphoton microscopy, and identical resolving capability with pure optical microscopies . The use of new scanner and fast laser sources promotes the development of compact and miniaturized ORPAMs, which are suitable for brain imaging and reveal the potential to carry out studies in awaking animals .…”

Section: Introductionmentioning

confidence: 99%

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In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Guo

Chen

et al. 2018

Journal of Biophotonics

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Brain imaging is an important technique in cognitive neuroscience. In this article, we designed a stereotaxic-apparatus-compatible photoacoustic microscope for the studies of rat cortical hemodynamics. Compared with existing optical resolution photoacoustic microscopy (ORPAM) systems, the probe owns feature of fast, light and miniature. In this microscope, we integrated a miniaturized ultrasound transducer with a center frequency of 10 MHz to detect photoacoustic signals and a 2-dimensional (2D) microelectromechanical system (MEMS) scanner to achieve raster scanning of the optical focus. Based on phantom evaluation, this imaging probe has a high lateral resolution of 3.8 μm and an effective imaging domain of 2 × 2 mm . Different from conventional ORPAMs, combining with standard stereotaxic apparatus enables broad studies of rodent brains without any motion artifact. To show its capability, we successfully captured red blood cell flow in the capillary, monitored the vascular changes during bleeding and blood infusion and visualized cortical hemodynamics induced by middle cerebral artery occlusion.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Guo

Chen

et al. 2018

Journal of Biophotonics

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“…Therefore, the PAM is a technology expected to have great utility in the diagnosis of epilepsy events. To the best of our knowledge, two forms of PA signals can be captured to detect epileptic seizures (65)(66)(67)(68)(69)(70)(71). The first is a PAI obtained by PAM (65)(66)(67)(68), and the second is a PA signal curve captures by PA sensor technology (69)(70)(71).…”

Section: Epileptic Seizurementioning

confidence: 99%

Photoacoustic imaging as a highly efficient and precise imaging strategy for the evaluation of brain diseases

Qiu¹,

Lan²,

Gao³

et al. 2021

Quant Imaging Med Surg

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Photoacoustic imaging (PAI) is an emerging imaging strategy with a unique combination of rich optical contrasts, high ultrasound spatial resolution, and deep penetration depth without ionizing radiation.Taking advantage of the features mentioned above, PAI has been widely applied to preclinical studies in diverse fields, such as vascular biology, cardiology, neurology, ophthalmology, dermatology, gastroenterology, and oncology. Among various biomedical applications, photoacoustic brain imaging has great importance due to the brain's complex anatomy and the variability of brain disease. In this review, we aimed to introduce a novel and effective imaging modality for diagnosing brain diseases. Firstly, a brief overview of two major types of PAI system was provided. Then, PAI's major preclinical applications in brain diseases were introduced, including early diagnosis of brain tumors, subtle changes in the chemotherapy response, epileptic activity and brain injury, foreign body, and brain plaque. Finally, a perspective of the remaining challenges of PAI was given for future advancements.

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“…Hu group proposed a conventional motorized‐scanner‐based photoacoustic microscope to image cerebral hemodynamics in awake mice restricted by an angle‐ and height‐adjustable head‐restraint apparatus . Xi et al developed a miniature hybrid probe integrating photoacoustic sensor and microelectrodes to study brain hemodynamics of freely moving rats . However, this technique is invasive and only able to visualize hemodynamics in one spatial point.…”

Section: Introductionmentioning

confidence: 99%

Wearable optical resolution photoacoustic microscopy

Chen

Xie

2019

Journal of Biophotonics

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Optical resolution photoacoustic microscopy (ORPAM) is an emerging imaging technique, which has been extensively used to study various brain activities and disorders of the anesthetized/restricted rodents with a special focus on the morphological and functional visualization of cerebral cortex. However, it is challenging to develop a wearable photoacoustic microscope, which enables the investigation of brain activities/disorders on freely moving rodents. Here, we report a wearable and robust optical resolution photoacoustic microscope (W-ORPAM), which utilizes a small, light, stable and fast optical scanner. This wearable imaging probe features high spatiotemporal resolution, large field of view (FOV) and easy assembly as well as adjustable optical focus during the in vivo experiment, which makes it accessible to image cerebral cortex activities of freely moving rodents. To demonstrate the advantages of this technique, we used W-ORPAM to monitor both morphological and functional variations of vasculature in cerebral cortex during the induction of ischemia and reperfusion of a freely moving rat.

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Hybrid photoacoustic and electrophysiological recording of neurovascular communications in freely-moving rats

Cited by 18 publications

References 56 publications

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Photoacoustic imaging as a highly efficient and precise imaging strategy for the evaluation of brain diseases

Wearable optical resolution photoacoustic microscopy

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