Hybrid plant regeneration from interfamilial somatic hybridization between grapevine (vitia vinifera L.) and Red Thorowax (Bupleurum scorzonerifolium willd)

Song, Xuhong; Xia, Guangmin; Zhou, Aifen; Bao, Xue-Zhen; Chen, Huimin

doi:10.1007/bf02886345

Cited by 6 publications

(4 citation statements)

References 7 publications

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“…Some of the reports on somatic hybrids have shown that the chromosome numbers of the hybrids were less than the sum of the chromosome numbers of the fusion parents (Song et al, 1999;Yue et al, 2001;Xiang et al, 2003a, b). Therefore, chromosome number or ploidy level is unsuitable to identify somatic hybrids alone in a reliable manner.…”

Section: Cytological Markersmentioning

confidence: 96%

“…Protoplast symmetric fusion between nightshade (A. belladonna) and tobacco (N. tabacum) gave rise to highly asymmetric and fertile somatic hybrids containing only one small chromosome of nightshade in addition to the whole tobacco genome (Babiychuk et al, 1992). It is proposed that spontaneous asymmetry was possibly necessary for normal division and morphogenesis of the hybrid cells derived from remote combinations (Chen et al, 1992;Liu et al, 1995b;Song et al, 1999).…”

Section: Inheritance Of Nuclear Genomes In Symmetric Fusionmentioning

confidence: 96%

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Intergeneric somatic hybridization and its application to crop genetic improvement

Liu

Deng

2005

Plant Cell Tiss Organ Cult

112

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Related or distant species of cultivated crops are a large pool of many desirable genes. Gene transfer from these species through conventional breeding is difficult owing to post-and pre-zygotic sexual incompatibilities. Somatic hybridization via protoplast fusion is a possible alternative for gene transfer from these species to cultivated crops. Since the early days of somatic hybridization many intergeneric somatic hybrids have been developed through symmetric fusion, asymmetric fusion and microfusion. Somatic hybrids are mainly selected by using markers such as specific media or fusion parents with special features, biochemical mutants, antibiotic resistance and complementation strategy. The hybridity of the regenerants is determined based on morphological, cytological and molecular analysis. The inheritance patterns of nuclear and cytoplasmic genomes in the somatic hybrids are diverse. Nuclear DNA from both fusion parents co-exists congruously in some hybrids with translocation and rearrangement of chromosomes, but spontaneous elimination of chromosomes from either or both fusion parents has been observed very often. In asymmetric fusion, chromosome elimination is an important issue that is a complicated process influenced by many factors, such as irradiation dose, phylogenetic relatedness, ploidy level of fusion parent and regenerants. As for chloroplast genome, uniparental segregation is mainly detected, though co-existence is also reported in some cases. The mitochondrial genome, in contrast to chloroplast, undergoes recombination and very frequent rearrangements. Somatic cell fusion has potential applications for crop genetic improvement by overcoming sexual incompatibility or reproductive barriers, and by realizing novel combinations of nuclear and/or cytoplasmic genomes.

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Section: Cytological Markersmentioning

confidence: 96%

Section: Inheritance Of Nuclear Genomes In Symmetric Fusionmentioning

confidence: 96%

Intergeneric somatic hybridization and its application to crop genetic improvement

Liu

Deng

2005

Plant Cell Tiss Organ Cult

112

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“…Hybrid plant regeneration always needs chromosomes elimination in some degree to reduce the imbalance of hereditary substances between both parents (Song et al 1999), but the elimination has a limitation. Although there are many factors influencing the chromosome elimination in somatic hybrids, but we suggest that the most important reason for chromosome elimination in tri-parental hybrids was hereditary imbalance.…”

Section: Gish Of Somatic Hybrids and Their Progeniesmentioning

confidence: 99%

Tri-parental protoplast fusion ofBrassicaspecies to produce somatic hybrids with high genetic and phenotypic variability

Lian¹,

Lin²,

Zheng³

2015

Intern. Jour. Contemp. Microbiol.

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Plating efficiencies and the plant regeneration frequencies of bi-parental fusion of Brassica campestris pekinensis with Ogura cytoplasmic male sterility (CMS), B. oleracea L. var. italica with Ogura CMS, and Brassica juncea var. crispifolia were compared for illustrating the advantages of tri-parental somatic hybridization. The results showed that in the triparental fusion combination, 73 plants regenerated from 712 calli and the plant regeneration frequency was 10.3%, three or four times that obtained with the 2 fusion combinations. These hybrids were classified into 12 types based on morphology, and most showed intermediate characteristics between 2 or more of the parental species. The somatic hybiridity were confirmed by flow cytometry, genomic in situ hybridization (GISH) and PCR analysis, indicating that these regenerated plants were all true hybrids. Most of the progenies with normal pollen showed varied number of seed set after backcrossing with B. juncea. The high variability in the hybrids obtained illustrated that somatic hybridization may be useful in broadening existing Brassica gene pools and obtaining material for breeding.

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“…(Umbelliferae) is a traditionally important Chinese herb used in the treatment of influenza, fever, malaria, and menstrual disorders in China (Chang and But 1987). Somatic hybrid plants were obtained by using B. scorzonerifolium as one partner (Song et al 1999;Wang et al 2005;Zhou et al 2006). In our former research, B. scorzonerifolium was considered as an interesting donor parent to produce hybrids that could enable its gene flow to the A. thaliana gene pool, so as to target the important genes in relation to the effect content of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Chromosomes are eliminated in the symmetric fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd.

Wang

Zhao

Peng

et al. 2007

Plant Cell Tiss Organ Cult

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In order to investigate chromosome elimination in symmetric somatic hybridization between Bupleurum scorzonerifolium and Arabidopsis thaliana, protoplasts were isolated from suspension cultures of both A. thaliana and B. scorzonerifolium parents. Biparental protoplasts were mixed at a rate of 1.5:1 and fused with PEG-method. After protoplast fusion, the products were cultured in the P5 liquid medium for microcallus formation. Single cell lines formed from microcalli after subculturing on the MB1 (Xia and Chen, Plant Sci 120:197-203, 1996) solid medium. The putative somatic hybrid cell lines were identified by cytological and molecular analysis. Of the 132 somatic cell lines generated, 16 were identified as somatic hybrids, with the phenotypes resembled B. scorzonerifolium parent. These hybrids showed a complete set of B. scorzonerifolium chromosome and 0-2 small chromosome(s) of A. thaliana. A few of them showed nuclear and cytoplasmic SSR fragments of A. thaliana. These hybrid cell lines could differentiate to green spots, buds/leaves through complementation of regeneration ability. The chromosomes elimination of A. thaliana was discussed.

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Hybrid plant regeneration from interfamilial somatic hybridization between grapevine (vitia vinifera L.) and Red Thorowax (Bupleurum scorzonerifolium willd)

Cited by 6 publications

References 7 publications

Intergeneric somatic hybridization and its application to crop genetic improvement

Intergeneric somatic hybridization and its application to crop genetic improvement

Tri-parental protoplast fusion ofBrassicaspecies to produce somatic hybrids with high genetic and phenotypic variability

Chromosomes are eliminated in the symmetric fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd.

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