Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis

Dehghani, Sima; Sabzehei, Faezeh; Taromchi, Amir Hossein; Mobaien, Ahmad Reza; Arsang‐Jang, Shahram

doi:10.1016/j.jim.2021.113123

Cited by 9 publications

(8 citation statements)

References 31 publications

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“…The study on omp22, omp 25 and omp 31 applied six possible linear B-cell epitopes in tandem and constructed a recombinant protein as diagnostic antigens. However, the accuracy of the recombinant protein is not as good as our full-length omps, 28 which may be due to the lack of some important epitopes in recombinant protein. On the other hand, the accuracy of omp combination with omp10, omp 19 and omp28 was 96.0%, which was higher than our combination in diagnosing cattle sera, 29 which is probably due to fewer samples used in our study.…”

Section: Discussionmentioning

confidence: 92%

“…Many studies have demonstrated that using Brucella omps such as omp10, omp16, omp19, omp25, omp28, omp31 and BP26 is very effective way for serological diagnosis of brucellosis. [26][27][28][29] We previously tested human, goat and cattle sera using individual omp as antigen, but the sensitivity of single omp was not as good as the conventional LPS antigen. 13 We hypothesized that the sensitivity of diagnosis could be improved by using multiple omps.…”

Section: Discussionmentioning

confidence: 99%

“…The combination of omp10, omp 19 and omp28 has ever been used to diagnose human brucellosis and combination of omp 22, omp 25 and omp 31 has also been used for the diagnosis of cattle brucellosis. 28,29 However, there are no studies showing that any combinations of omps can be simultaneously used for human, goat and cattle brucellosis. The study on omp22, omp 25 and omp 31 applied six possible linear B-cell epitopes in tandem and constructed a recombinant protein as diagnostic antigens.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis

Yao¹,

Guo²,

Wu³

et al. 2022

IDR

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Background Brucellosis is a zoonotic disease that causes substantial public health problems and endangers the development of animal husbandry in endemic areas. Early diagnosis of infected animals and humans is a crucial step in reducing the incidence of brucellosis. In this study, we designed different combinations of Brucella major outer membrane proteins (omps) including omp10, omp16, omp19, omp25, omp31 and BP26 as antigens and evaluated their efficiency in serodiagnosis for brucellosis. The efficiency assay was conducted using the method of indirect enzyme-linked immunosorbent assay (iELISA) together with a collection of brucellosis-positive sera and healthy sera from multiple species (161 from human, 120 from goat and 144 from cattle). The diagnostic effectiveness of each omp combination was analyzed by receiver operating characteristic (ROC) curve with the software GraphPad Prism version 6.05. Results The omp25/omp31/BP26 combination showed the best efficiency in diagnosis for human brucellosis. The area under the ROC curve (AUC) was 0.995 and, compared with the serum tube agglutination test (SAT) and the Rose Bengal plate agglutination test (RBPT), the positive and negative diagnostic accuracies of iELISA were 94.59% (105/111) and 100.0% (50/50), respectively. Evaluation of the 120 goat and 144 cattle serum samples showed that the best combination for diagnosing both omp31/BP26, the AUC was 0.9262 in goat and 0.9344 in cattle, and compared with those of SAT and RBPT, the positive and negative diagnostic accuracies in goat were 72.73% (48/66) and 100.0% (54/54), respectively. The positive and negative diagnostic accuracies in cattle were 79.79% (75/94) and 100.0% (50/50), respectively. Cross-reaction assays showed that omp25/omp31/BP26 and omp31/BP26 do not cross with other common pathogens. Conclusion The results indicated that combinations of omps, as protein antigens, can be used to diagnose brucellosis with high accuracy in human, goat and cattle.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis

Yao¹,

Guo²,

Wu³

et al. 2022

IDR

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“…Another approach to improve the sensitivity and specificity of an ELISA test is the utilization of multi-epitope recombinant protein as antigen [ 17 , 18 ]. For example, Yao et al (2022) used OMP19 in different combination with Brucella outer membrane proteins in the serodiagnosis of brucellosis: (OMP10, OMP16, OMP19) and (OMP10, OMP16, OMP19, OMP25, OMP31 and BP26) [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…These researchers suggested that the best OMP combination was omp25, omp31 and BP26 [ 17 ]. Dehghani et al (2021) selected Brucella OMP22, OMP25, and OMP31 antigens and hybridized them with the rigid KP linker (K = Lysine, P = Proline) [ 18 ]. They evaluated their assembled antigen for diagnosis of brucellosis in 37 patients and 27 healthy individuals by ELISA tool.…”

Section: Discussionmentioning

confidence: 99%

Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen

Golchin,

Mollayi,

Mohammadi

et al. 2023

BMC Biotechnol

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Background Brucellosis remains one of the global health concerns that reemerges in recent years. Delayed or inaccurate diagnosis end to a long treatment duration and financial burden; therefore, finding a good antigen for detection of specific anti-Brucella antibodies is crucial. We intended to evaluate the serodiagnosis value of recombinant Brucella outer membrane protein 19 kDa (rOMP19) using indirect ELISA system compared with Rose Bengal test. Results The OMP19 sequence was successfully cloned into pET-28a and produced in E. coli cells (DE3). After extraction and purification of rOMP19, this protein was used for designing indirect ELISA to detect anti-Brucella antibodies in 73 human sera, including 6 brucellosis-positive and 67 brucellosis-negative samples. The accuracy of rOMP19 ELISA was evaluated by receiver operating characteristic (ROC) curve and then compared with Rose Bengal plate test and a commercial anti-IgG Brucella ELISA kit. In comparison with Rose Bengal plate test, the area under the ROC curve was 0.985 (95% CI, 0.96–1.00). From coordinates of the curve, the optimal cut-off value was selected at 0.147, in which the diagnostic sensitivity was 100%, and the specificity was 94%. At this cut-off point, 10 samples were diagnosed as positive (6 true positives and 4 false positives), while negative samples were all correctly diagnosed. The results of our designed rOMP19 ELISA was the same as data obtained from commercial ELISA kit, which applied LPS as an antigen. Conclusions We concluded that OMP19 is an efficient antigen for the serodiagnosis of human brucellosis.

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Expression of cytokine and Apoptosis-Associated genes in mice bone Marrow-Derived Macrophages stimulated with Brucella recombinant type IV secretion effectors

Li,

Wang,

Han

et al. 2024

Cytokine

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Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis

Cited by 9 publications

References 31 publications

Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis

Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis

Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen

Expression of cytokine and Apoptosis-Associated genes in mice bone Marrow-Derived Macrophages stimulated with Brucella recombinant type IV secretion effectors

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