Hybrid recreation by reverse breeding in Arabidopsis thaliana

Wijnker, Erik; Deurhof, Laurens; Belt, José van de; Snoo, C. Bastiaan de; Blankestijn, Hetty; Becker, Frank; Ravi, Maruthachalam; Chan, Simon W. L.; Dun, Kees van; Lelivelt, C. L. C.; Jong, Hans de; Dirks, R.; Keurentjes, Joost J. B.

doi:10.1038/nprot.2014.049

Cited by 43 publications

(37 citation statements)

References 27 publications

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“…Furthermore, the HI parent produces few viable pollen 16,23 and thus we prefer to use HI as a female parent in crosses to produce haploids. F1 seed from HI Â WT crosses produce a mixture of hybrid diploids, aneuploids and haploids in addition to a high frequency of aborted seeds 16,24 .…”

Section: Resultsmentioning

confidence: 99%

“…Haploids can be identified by phenotypic, genotypic and cytological methods 16,24 (see Methods, Supplementary Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the haploid plants can also be PCR tested for absence of the GFP-tailswap construct. For details on the primers and methodology for PCRbased genotyping, please refer to an earlier publication 24 . Final confirmation of haploids can be done by counting the number of chromosomes either in mitosis or meiosis ( Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

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A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

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Section: Resultsmentioning

confidence: 99%

“…Haploids can be identified by phenotypic, genotypic and cytological methods 16,24 (see Methods, Supplementary Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

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A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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“…The suppression of meiotic crossovers and transmission of non-recombinant chromosomes to haploid gametes is a key to reverse breeding. Gametes are subsequently regenerated as doubled-haploid offspring among which the parental lines are selected (Wijnker et al, 2014). Thanks to the knowledge of the oil palm gene space (Low et al, 2014), meiosis regulators, such as DMC1orthologs (Wijnker et al, 2012) can be identified and then controlled.…”

Section: Increasing Oil Palm Productivitymentioning

confidence: 99%

Oil palm natural diversity and the potential for yield improvement

et al. 2015

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African oil palm has the highest productivity amongst cultivated oleaginous crops. Species can constitute a single crop capable to fulfill the growing global demand for vegetable oils, which is estimated to reach 240 million tons by 2050. Two types of vegetable oil are extracted from the palm fruit on commercial scale. The crude palm oil and kernel palm oil have different fatty acid profiles, which increases versatility of the crop in industrial applications. Plantations of the current varieties have economic life-span around 25–30 years and produce fruits around the year. Thus, predictable annual palm oil supply enables marketing plans and adjustments in line with the economic forecasts. Oil palm cultivation is one of the most profitable land uses in the humid tropics. Oil palm fruits are the richest plant source of pro-vitamin A and vitamin E. Hence, crop both alleviates poverty, and could provide a simple practical solution to eliminate global pro-vitamin A deficiency. Oil palm is a perennial, evergreen tree adapted to cultivation in biodiversity rich equatorial land areas. The growing demand for the palm oil threatens the future of the rain forests and has a large negative impact on biodiversity. Plant science faces three major challenges to make oil palm the key element of building the future sustainable world. The global average yield of 3.5 tons of oil per hectare (t) should be raised to the full yield potential estimated at 11–18t. The tree architecture must be changed to lower labor intensity and improve mechanization of the harvest. Oil composition should be tailored to the evolving needs of the food, oleochemical and fuel industries. The release of the oil palm reference genome sequence in 2013 was the key step toward this goal. The molecular bases of agronomically important traits can be and are beginning to be understood at the single base pair resolution, enabling gene-centered breeding and engineering of this remarkable crop.

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“…So konnten im Rahmen einer Proof-of-Concept-Studie anhand der Modellpflanze Arabidopsis thaliana homozygote Elternlinien aus einem Hybridindividuum gewonnen werden (Wijnker et. al., 2012;Wijnker et. al., 2014).…”

Section: Reverse Breedingunclassified

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

Müller‐Röber¹,

Marx-Stölting²

2015

Dritter Gentechnologiebericht

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Hybrid recreation by reverse breeding in Arabidopsis thaliana

Cited by 43 publications

References 27 publications

A haploid genetics toolbox for Arabidopsis thaliana

A haploid genetics toolbox for Arabidopsis thaliana

Oil palm natural diversity and the potential for yield improvement

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

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