This paper presents the investigation of relative sensitivity profile of Alcohol through Photonic Crystal fiber at different temperature. Here, 15%, 40%, 60%, 75% of Ethyl Alcohol-water mixture is inserted through the core of Photonic crystal fiber at temperature like 20˚C, 25˚C and 30˚C. COMSOL Multiphysics is used as simulation software and the simulation process is done at wavelength range 600 nm to 1600 nm. From this work, the relative sensitivity is obtained approximately 44, 44.59, 44.85, 45 in percentage at temperature 20˚C, 42, 44.2, 44.8, 44.9 in percentage at temperature 25˚C, and 42, 43.8, 44.5, 44.85 in percentage at temperature 30˚C for 15%, 40%, 60%, 75% of Ethyl Alcohol-water mixture at wavelength 1500 nm respectively. Again, higher sensitivity is achieved when this sensor is operated at lower temperature.