Hybridisation in a tropical seagrass genus, Halodule (Cymodoceaceae), inferred from plastid and nuclear DNA phylogenies

Yu, I.; Tanaka, Norio

doi:10.7751/telopea20116015

Cited by 25 publications

(15 citation statements)

References 31 publications

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“…Halodule pinifolia and H. uninervis were resolved as distinct species. Taxonomic segregation of Halodule pinifolia from H. uninervis is not clearly resolved based on trnL (Waycott et al, 2006), single rbcL (Ito & Takahata, 2011) and rbcL/matK barcode (Lucas et al, 2012). In addition, a hybrid in the genus Halodule based on phyB analyses was detected (Ito & Takahata, 2011).…”

Section: Discussionmentioning

confidence: 91%

“…Taxonomic segregation of Halodule pinifolia from H. uninervis is not clearly resolved based on trnL (Waycott et al, 2006), single rbcL (Ito & Takahata, 2011) and rbcL/matK barcode (Lucas et al, 2012). In addition, a hybrid in the genus Halodule based on phyB analyses was detected (Ito & Takahata, 2011). However, the recent analysis of two plastid (matK, rbcL) and four mitochondrial genes (atp1, ccmB, cob, nad5) indicated that H. uninervis and H. pinifolia are distinct species (Petersen et al, 2014).…”

Section: Discussionmentioning

confidence: 96%

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New insights into DNA barcoding of seagrasses

Nguyen

Höfler

Glasenapp

et al. 2015

Systematics and Biodiversity

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Taxonomists find some plant genera challenging because of the few morphological differences or unclear characters among closely related species, which leads to the misidentification of taxa. DNA barcoding is an approach to identify species by using short orthologous DNA sequences, known as 'DNA barcodes'. Concatenated rbcL and matK sequences are considered DNA barcodes for seagrasses. However, these markers are not applicable to all members of seagrasses at the species level, especially within the genus Halophila. Our previous studies indicated that the internal transcribed spacer (ITS) showed higher species resolution than the concatenated rbcL and matK sequences in the case of Halophila ovalis and closely related species. In this study, 26 ITS, two rbcL and two matK consensus sequences from 18 seagrass taxa belonging to four families collected in India, Vietnam, Germany, Croatia and Egypt were processed. Molecular ITS analysis resolved five clades. The results also indicate that the Cymodoceaceae family might be a non-monophyletic group. In conclusion, ITS could be applied as a DNA barcode for seagrasses instead of the rbcL/matK system previously proposed.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 96%

New insights into DNA barcoding of seagrasses

Nguyen

Höfler

Glasenapp

et al. 2015

Systematics and Biodiversity

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“…Either of these reasons may cause incongruence between ITS and plastid phylogenies. Among marine angiosperms, natural hybridization has been observed in only four genera (Halodule, Ruppia, Posidonia, and Zostera) (Ito & Tanaka, 2011;Coyer et al, 2008;Martínez-Garrido et al, 2016;Sinclair, Cambridge & Kendrick, 2019). Ito & Tanaka (2011) found sympatric Halodule uninervis and H. pinifolia hybridizing in the waters of Okinawa by reconstructing their phylogenetic relationship with rbcL and psbA-trnH loci.…”

Section: Discussionmentioning

confidence: 99%

Genetic identification and hybridization in the seagrass genusHalophila(Hydrocharitaceae) in Sri Lankan waters

Liu

Kumara

Hsu

2020

PeerJ

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Seagrasses, as marine angiosperms, play important roles in coastal ecosystems. With increasing anthropogenic impacts, they are facing dramatic declines on a global scale. Halophila is well-known as a complex taxonomic challenge mainly due to high morphological plasticity. By using only a morphological approach, the genus could be over-split or similar species could be erroneously lumped, thus masking its true biodiversity. In the present study, we incorporated genetic identification with morphological examination to reveal the identity of Halophila plants in southern and northwestern Sri Lankan waters. The nuclear ribosomal internal transcribed spacer (ITS) region and chloroplast ribulose-bisphosphate carboxylase gene (rbcL) were used to identify plants collected from the Gulf of Mannar, Puttalam Lagoon, and Matara, Sri Lanka. Based on genetic identification, H. major (Zoll.) Miquel is reported for the first time from Sri Lanka, which might have been misidentified as H. ovalis in previous literature based on morphology alone. We also observed a first hybridization case of Halophila cross between H. ovalis and H. major. Two potential cryptic species were found, herein designated Halophila sp. 1 (allied to H. minor) and Halophila sp. 2 (closely related to H. decipiens). In order to clarify taxonomic ambiguity caused by morphological plasticity and the low resolution of genetic markers, further comparative phylogenomic approaches might be needed to solve species boundary issues in this genus.

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“…This phenomenon is known to facilitate their resistance to global change in different ecological niches [47][48][49][50][51]. Probably, the origins of morphotype for a species, is intraspecific polymorphism, speciation in progress, incomplete derivation sorting or hybridization through introgression [52][53] reported for the Cymodoceaceae family [54].…”

Section: Discussionmentioning

confidence: 99%

Morphology and Genetic Studies of Cymodocea Seagrass Genus in Tunisian Coasts

Bchir¹,

Djellouli²,

Zitouna³

et al. 2019

Phyton

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Specimens of Cymodocea (Viridiplantae, Magnoliophyta) collected on the Tunisian coasts showed a particular morphological and anatomical difference with the classical descriptions of Cymodocea nodosa (Ucria) Asch. the only species of this genus reported in the Mediterranean Sea. In order to precise the taxonomic identity of the new specimens we aimed in this work (i) to verify the identity of the new forms, (ii) to evaluate the genetic diversity of the population, (iii) to test the validity of the existing identification keys of the Tunisian Cymodocea populations. Four stations located in two regions of the Tunisian coasts were sampled. Leaf morphological and anatomical characters used in taxonomic identification were measured (e.g., number of cross veins, shape of the apex). The genetic study was performed using three most common chloroplast markers for plant characterization (DNA barcodes rbcL, matK and trnHpsbA). The morphological study revealed the presence of three C. nodosa morphotypes, described here for the first time, while the molecular characterization did not allow the discrimination of these morphological types. In regard to these results, it would be wise to review the classical identification keys of the Cymodocea genus.

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Hybridisation in a tropical seagrass genus, Halodule (Cymodoceaceae), inferred from plastid and nuclear DNA phylogenies

Cited by 25 publications

References 31 publications

New insights into DNA barcoding of seagrasses

New insights into DNA barcoding of seagrasses

Genetic identification and hybridization in the seagrass genusHalophila(Hydrocharitaceae) in Sri Lankan waters

Morphology and Genetic Studies of Cymodocea Seagrass Genus in Tunisian Coasts

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