Hybridization Assay at a Disposable Electrochemical Biosensor for the Attomole Detection of Amplified Human Cytomegalovirus DNA

DNA Electrochemical Behaviors, Recognition and Sensing by Combining with PCR Technique

“…Thirdly, association with other technologies such as immunological and DNA microarrays and chips etc will provide us further bio-information on the interaction between DNA and proteins or drugs, multiple DNA sequences and so on. The amplification methods such as PCR have been used to amplify the target DNA [54,72,74,81,87,104,111]. With this technique an approach for sensitive detection of femtogram original genomic target DNA can be achieved [126,141,142].…”

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

“…Only a few authors have reported the coupling of biosensors with a DNA amplification method (e.g. PCR) to obtain reliable measurements of clinical interest (Marrazza et al, 2000;Azek et al, 2000;Authier et al, 2001;Meric et al, 2002;Lucarelli et al, 2002). Direct, enzyme-based, detection of DNA or RNA with no PCR pre-amplification has rarely been reported (Downs et al, 1988;Wojciechowski et al, 1999).…”

“…An electrochemical enzyme-amplified hybridisation assay for the detection of human cytomegalovirus DNA in PCR samples was described by Brossier's group (Azek et al, 2000). The biosensor format involved: (a) adsorption of denatured PCR products (target) onto the sensing area of a screen-printed carbon electrode; (b) hybridisation with a short, biotinylated, DNA probe; (c) hybrid labelling with a streptavidin-conjugated HRP; (d) differential pulse voltammetric detection of the enzyme-generated product.…”

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

“…171,172 Azek et al demonstrated the sensitivity of this technique with their enzyme label-based biosensor. 173 The group used a peroxidase enzyme label and a screen-printed carbon electrode to detect DNA sequences in human Cytomegalovirus. The limit of detection of the target was 0.6 amol/mL, making the technique 83 times more sensitive than standard hybridization techniques using colorimetric methods.…”