Hybridization assay of hepatitis B virus by QCM peptide nucleic acid biosensor

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

“…With this objective, different groups have demonstrated that PNA probes immobilized on a QCM transducer enable screening of functionally relevant single mutations of the p53 gene [48,66]. In another recent application, Yao et al [67] constructed a PNA-based, QCM biosensor for label-free and real-time monitoring of the hybridization of hepatitis B virus (HBV) genomic DNA without previous PCR amplification. The detection limit (three times the noise signal) was 8.6×10 −12 g L −1 and, working with clinical samples, the specificity was found to be extremely high (94.44%) compared with the reference method of real time PCR.…”

Tackling the Problem of Tuberculosis by Nanotechnology

“…There is increasing demand for biosensor technology for fast and precise detection of TB with high affinity and specificity. Biosensor technology has the potential to provide a qualitative and quantitative analysis and is free from radioactive or fluorescent tags (Tombelli et al, 2000, Zhou et al, 2001Yao et al, 2008).…”

A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

“…The quantification of the hybridisation efficiency was ensured by fluorescence (Chakrabarti & Klibanov, 2003). In a very recent study, hybridisation of hepatitis B virus by PNA was detected by QCM with a detection limit of 8.6 pg/L (Yao et al, 2008). This work relied on the immobilisation of PNA probes on a gold quartz using Carruso et al method, grafting of a thiol on which avidin was chemically bound and then the biotinylated PNA probe (Carruso et al, 1997).…”

“…Basic Principle Sensitivity Reference 0.1 pM (Su et al, 2008) 0.7 pM (Endo et al, 2005) SPR Change in the refractive index upon hybridisation A few nM (Liu et al, 2006) 8.6pg/L (Yao et al, 2008) 5 μM (Ananthanawat et al, 2009) 10 μg/mL QCM Change in the resonance frequency of a quartz 1 μM (Höök et al, 2001) SERS Enhanced electromagnetic field on nanostructured surfaces 1 pM ToF SIMS Detection of PO 2 -and PO 3 -fragments (Arlinghaus & Kwoka, 1997;Brandt et al, 2003 techniques in the domain. To our view, more important than sensitivity that very much depends on the measurement conditions and, in some cases, types of labelling, the use of synthetic PNA, instead of DNA, offers a remarkable specificity to a single mismatched oligonucleotides ; this is critical for many gene desease recognition.…”