Hybridization in Hydrophiles: Natural Interspecific Hybrids in <I>Najas</I> (Hydrocharitaceae)

Through thick and thin: Cryptic sympatric speciation in the submersed genus Najas (Hydrocharitaceae)

“…Several species exhibit wide distributional ranges, and the genus is characterized by extensive inter-and intraspecific polyploidy (Triest, 1988;Les and Philbrick, 1993). In particular, our attention has focused on Najas flexilis, a common temperate North American species that extends into northern Eurasia (Triest, 1988;Les et al, 2010). The species is distributed widely across northern North America (Haynes, 2000), where it occurs predominantly above the last Pleistocene glacial boundary, but is quite rare in Eurasia despite a rather extensive fossil record there (Fernald, 1923;Backman, 1948;Hultén, 1958).…”

Najas minor (Hydrocharitaceae) in North America: A reappraisal

“…2). Because these combined loci effectively delimit all other Najas species examined to date (Les et al, 2010(Les et al, , 2015, we concluded that all of our material belonged to a single species (N. minor), despite differences in anther locule number, which has been reported to vary within other Najas species (Cowie et al, 2000).…”

“…Amplification and sequencing of nrITS and cpDNA (trnK 5' intron; matK and rbcL coding regions) followed Les et al (2010). The nrITS region (most variable), was sequenced for all accessions.…”

“…The tree was rooted with N. gracillima as outgroup following Les et al (2010). Internal support was determined by bootstrap analysis with raxMLGUI (1000 replicates; bootstrap + consensus option).…”

A New Genus and New Combinations in Australian Villarsia (Menyanthaceae)

“…Les et al 2009Les et al , 2010 have provided straightforward evidence of hybridization (i.e. both alleles of extant parental species or recombinants thereof are separable using molecular cloning methods).…”