Hybridization in staminate and pistillate Salix alba and S. fragilis (Salicaceae): morphology versus RAPDs

Triest, Ludwig

doi:10.1007/s006060170062

Cited by 29 publications

(24 citation statements)

References 15 publications

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“…Based on morphology, both balanced S. alba-S. fragilis hybrids and introgressive hybrids, deriving from repeated backcrossing of hybrids to parental types, seem to dominate in the field (De Bondt, 1996;Triest et al, 2000). The analysis of leaves, buds and twigs have indicated that many potential hybrids are present within natural populations (Triest, 2001). This finding agrees with the observations of several botanists (Rechinger, 1964;Skvortsov, 1973;De Langhe et al, 1983;Meikle, 1992) that the vast majority of willows in the S. alba-S. fragilis complex could be hybrids or introgressants.…”

Section: Introductionmentioning

confidence: 99%

“…The latter type of markers offers an almost unlimited supply of molecular traits for distinctive fingerprinting of plant materials without prior knowledge of target DNA sequences. The area of willow research showing the greatest development with respect to the use of molecular marker technology is that of population genetics (Beismann et al, 1997;Triest et al, 1999Triest et al, , 2000Triest, 2001), whereas information on basic genomics is scanty (Thibault, 1997;Triest et al, 1997;Alstrom-Rapaport et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Linkage mapping in tetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids

et al. 2003

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Salix albaÀSalix fragilis complex includes closely related dioecious polyploid species, which are obligate outcrossers. Natural populations of these willows and their hybrids are represented by a mixture of highly heterozygous genotypes sharing a common gene pool. Since nothing is known about their genomic constitution, tetraploidy (2n ¼ 4x ¼ 76) in willow species makes basic and applied genetic studies difficult. We have used a two-way pseudotestcross strategy and singledose markers (SDMs) to construct the first linkage maps for both pistillate and staminate willows. A total of 242 amplified fragment length polymorphisms (AFLPs) and 50 selective amplifications of microsatellite polymorphic loci (SAMPL) markers, which showed 1:1 segregation in the F 1 mapping populations, were used in linkage analysis. In S. alba, 73 maternal and 48 paternal SDMs were mapped to 19 and 16 linkage groups covering 708 and 339 cM, respectively. In S. fragilis, 13 maternal and 33 paternal SDMs were mapped in six and 14 linkage groups covering 98 and 321 cM, respectively. For most cosegregation groups, a comparable number of markers linked in coupling and repulsion was identified. This finding suggests that most of chromosomes pair preferentially as occurs in allotetraploid species exhibiting disomic inheritance. The detection of 10 pairs of marker alleles from single parents showing codominant inheritance strengthens this hypothesis. The fact that, of the 1122 marker loci identified in the two male and female parents, the vast majority (77.5%) were polymorphic and as few as 22.5% were shared between parental species highlight that S. alba and S. fragilis genotypes are differentiated. The highly difference between S. alba-and S. fragilis-specific markers found in both parental combinations (on average, 65.3 vs 34.7%, respectively) supports the (phylogenetic) hypothesis that S. fragilis is derived from S. alba-like progenitors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Linkage mapping in tetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids

et al. 2003

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“…In previous studies of S. alba and S. fragilis, a higher differentiation of these two species was reported by using DNA markers as compared to morphological traits (Triest 2001). It has been suggested that this might be a consequence of the allotetraploid nature of the genomes of S. alba and S. fragilis, with the two species sharing one common or two highly related diploid ancestor species, but differentiated from each other by the genomic contributions of divergent second diploid ancestor species (Barcaccia et al 2014).…”

Section: Discussionmentioning

confidence: 89%

“…There is evidence that interspecific hybridisation is common between a range of Salix species (Palmé et al 2003), and controlled crosses have shown that S. alba and S. fragilis can also readily hybridise (Triest et al 2000). Additionally, continuous variation of morphological characteristics between these species has been reported in natural populations (Triest 2001). Despite this morphological continuum between the two species, DNA marker studies have indicated that the two species are clearly differentiated, suggesting that introgressive hybridisation is not widespread (Triest 2001, Oberprieler et al 2013.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, continuous variation of morphological characteristics between these species has been reported in natural populations (Triest 2001). Despite this morphological continuum between the two species, DNA marker studies have indicated that the two species are clearly differentiated, suggesting that introgressive hybridisation is not widespread (Triest 2001, Oberprieler et al 2013.…”

Section: Introductionmentioning

confidence: 99%

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Genetic analysis of Latvian Salix alba L. and hybrid populations using nuclear and chloroplast DNA markers

Ruņģis¹,

Laiviņš²,

Gailīte³

et al. 2017

iForest

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Latvia is at the northern border of the species range of S. alba L. in Europe, and there has been some debate whether the Latvian populations of S. alba are autochthonous, as this species has long been planted in Latvia as an ornamental tree in gardens, parks and roadsides. In addition, there is increasing interest in the use of several Salix species (including S. alba) as bioenergy crops. Natural S. alba stands throughout Latvia, as well as stands of possibly hybrid origin were analysed using nuclear and chloroplast markers. Our results showed that S. alba populations are probably natural, and that the rate of vegetative reproduction is low, supporting the evidence that Latvia is within the natural range of S. alba. These results provide the basis for the identification of possibly introduced or artificially regenerated stands of S.alba in Latvia. In addition, our results confirm that S. alba hybridises with S. fragilis, and that natural stands including hybrid individuals can be established. The analysis of chloroplast markers indicated that the predominant hybridisation occurs by fertilisation of S. fragilis by S. alba pollen; however, the extent of haplotype sharing between these two species should be further investigated.

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Genetic and phenotypic differentiation between invasive and native Rhododendron (Ericaceae) taxa and the role of hybridization

Erfmeier

Tsaliki

Ross

et al. 2011

Ecology and Evolution

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Hybridization has been repeatedly put forward to explain the invasiveness of Rhododendron ponticum L. in the British Isles. The present study investigates the pattern of ecotypic differentiation and hybridization among native North American R. catawbiense and R. maximum, native R. ponticum from Georgia and Spain, and invasive R. ponticum from Ireland and aims to assess the contribution of hybridization for Rhododendron invasion in the British Isles. Six populations per taxon were analyzed with AFLP markers for genetic dissimilarity, subjected to germination and growth experiments, and tested for frost hardiness. We assessed variation in morphological and ecological characteristics to identify traits displaying evidence of hybridization, thus, promoting invasiveness. Molecular marker analyses revealed a clear distinction between North American R. catawbiense and R. maximum on the one hand, and all R. ponticum populations on the other hand, displaying a complete intermixture of native Spanish and invasive Irish populations. Multivariate analyses of traits revealed leaf length–width ratio, relative growth rates (RGRs) in leaf length, root biomass, and shoot–root ratio to significantly discriminate between the different taxa and unequivocally assigned invasive Irish R. ponticum to the Spanish phenotypes. While the Irish R. ponticum had similar growth traits as conspecific native R. ponticum provenances, germination and biomass allocation were more similar to North American R. catawbiense and R. maximum. Hybridization did not contribute to explaining invasiveness of R. ponticum in Ireland. The similarity in germination and biomass allocation of invasive Irish R. ponticum and North American species has evolved independently and can more probably be attributed to an independent shift within the Ponticum cluster in Ireland.

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Hybridization in staminate and pistillate Salix alba and S. fragilis (Salicaceae): morphology versus RAPDs

Cited by 29 publications

References 15 publications

Linkage mapping in tetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids

Linkage mapping in tetraploid willows: segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecific hybrids

Genetic analysis of Latvian Salix alba L. and hybrid populations using nuclear and chloroplast DNA markers

Genetic and phenotypic differentiation between invasive and native Rhododendron (Ericaceae) taxa and the role of hybridization

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