Recently, it has been shown that the chiral magnetic insulator Cu 2 OSeO 3 hosts skyrmions in two separated pockets in temperature and magnetic field phase space. It has also been shown that the predominant stabilization mechanism for the low-temperature skyrmion (LTS) phase is via the crystalline anisotropy, opposed to temperature fluctuations that stabilize the well-established high-temperature skyrmion (HTS) phase. Here, we report on a detailed study of LTS generation by field cycling, probed by GHz spin dynamics in Cu 2 OSeO 3 . LTSs are populated via a field cycling protocol with the static magnetic field applied parallel to the 100 crystalline direction of plate and cuboid-shaped bulk crystals. By analyzing temperature-dependent broadband spectroscopy data, clear evidence of low-temperature skyrmion excitations with clockwise (CW), counterclockwise (CCW), and breathing mode (BR) character at temperatures below T = 40 K are shown. We find that the mode intensities can be tuned with the number of field-cycles below the saturation field. By tracking the resonance frequencies, we are able to map out the field-cyclegenerated LTS phase diagram, from which we conclude that the LTS phase is distinctly separated from the high-temperature counterpart. We also study the mode hybridization between the dark CW and the BR modes as a function of temperature. By using two Cu 2 OSeO 3 crystals with different shapes and therefore different demagnetization factors, together with numerical calculations, we unambiguously show that the magnetocrystalline anisotropy plays a central role for the mode hybridization.