We demonstrate matterwave interference in a warm vapor of rubidium atoms. Established approaches to light pulse atom interferometry rely on laser cooling to concentrate a large ensemble of atoms into a velocity class resonant with the atom optical light pulse. In our experiment, we show that clear interference signals may be obtained without laser cooling. This effect relies on the Doppler selectivity of the atom interferometer resonance. This interferometer may be configured to measure accelerations, and we demonstrate that multiple interferometers may be operated simultaneously by addressing multiple velocity classes.The technique of light pulse atom interferometry (LPAI) has proved to be exceptionally useful for precision acceleration measurements. Since its inception [1], research has branched into pursuits of inertial sensor technology [2-6] and foundational precision measurements [7][8][9][10], including space-based gravity wave detectors [11]. These demonstrations build upon well-vetted techniques in the field of laser cooling and trapping [12]. Reducing the velocity distribution of a large ensemble of atoms and collecting them into a well-defined spatial location affords ample time for interrogation [13,14] and high fidelity detection [15]. In this setting, the matter wave of each atom evolves with inertial freedom such that photon recoils may be used to coherently split and recombine the wave packets without perturbation. The experimental overhead is laser system complexity and ultra-high vacuum requirements that have challenged efforts fielding these instruments [14,[16][17][18][19][20].The simplicity of a vapor cell approach, used for atomic clocks [21] and magnetometry [22,23], is an alluring alternative. In this approach, long interrogation times are achieved through the use of a buffer gas or a spin antirelaxation coating. As such, multiple collisions occur between the interrogated atom and the buffer gas or cell coating over the duration of one measurement period. Such collisions spoil the inertial purity of the wave packets and would obfuscate the LPAI fringe. Nevertheless, by borrowing certain aspects of the vapor cell approach, namely a spin anti-relaxation coating for state preparation, and blending this with the inherent velocity-filtering function of the photon recoil in LPAI, we re-imagine atom interferometry. Consequently, we achieve high fidelity interference signals in a significantly simplified warm vapor experiment, without laser cooling.LPAI uses two-photon stimulated Raman transitions between hyperfine ground states (e.g. |F = 1 and |F = 2 in 87 Rb) to create coherent superpositions of momentum states with the effect of redirecting matter wave packets to form the atom optical elements of beam splitter and mirror. When the two optical fields are ar- (Color online) Vapor interferometer concept-not to scale. The 2-D mesh Gaussian represents the MaxwellBoltzmann distribution in cylindrical coordinates z and ρ for room temperature atoms in |F = 1 . The solid blue Sinc functions are two na...