Hydration of pectic polysaccharides

Ryden, Peter; MacDougall, Alistair J.; Tibbits, C.William; Ring, Stephen G.

doi:10.1002/1097-0282(200011)54:6<398::aid-bip40>3.0.co;2-g

Cited by 71 publications

(52 citation statements)

References 37 publications

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“…For more highly charged polymers, minimal swelling was observed because of molecular collapse. Replacement of Na + by Ca 2+ cations resulted in minimal swelling and the formation of a network even for weakly charged pectic polysaccharides (Ryden et al 2000). In cell walls, NMR Fig.…”

Section: Discussionmentioning

confidence: 97%

“…Model concentrated systems (films prepared by dispersing pectin molecules in water) have allowed in vitro studies of the swelling properties at different pH, ionic strengths as a function of osmotic stress (Ryden et al 2000). For weakly charged polymers at low concentration of monovalent salt, the swelling of pectic polysaccharides increased with reduction of osmotic pressure, behaviour characteristic of a polyelectrolyte effect.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of pectic epitopes in cell walls of the sugar beet root

Guillemin

Guillon

Bonnin

et al. 2005

Planta

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Immunolabelling techniques with antibodies specific to partially methyl-esterified homogalacturonan (JIM5: unesterified residues flanked by methylesterified residues. JIM7: methyl-esterified residues flanked by unesterified residues), a blockwise de-esterified homogalacturonan (2F4), 1,4-galactan (LM5) and 1,5-arabinan (LM6) were used to map the distribution of pectin motifs in cell walls of sugar beet root (Beta vulgaris). PME and alkali treatments of sections were used in conjunction with JIM5-7 and 2F4. The JIM7 epitope was abundant and equally distributed in all cells. In storage parenchyma, the JIM5 epitope was restricted to some cell junctions and the lining of intercellular spaces while in vascular tissues it occurred at cell junctions in some phloem walls and in xylem derivatives. After secondary wall formation, the JIM5 epitope was restricted to inner cell wall regions between secondary thickenings. The 2F4 epitope was not detected without de-esterification treatment. PME treatments prior to the use of 2F4 indicated that HG at cell corners was not acetylated. The LM5 epitope was mainly present in the cambial zone and when present in storage parenchyma, it was restricted to the wall region closest to the plasma membrane. The LM6 epitope was widely distributed throughout primary walls but was more abundant in bundles than in medullar ray tissue and storage parenchyma. These data show that the occurrence of oligosaccharide motifs of pectic polysaccharides are spatially regulated in sugar beet root cell walls and that the spatial patterns vary between cell types suggesting that structural variants of pectic polymers are involved in the modulation of cell wall properties.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Distribution of pectic epitopes in cell walls of the sugar beet root

Guillemin

Guillon

Bonnin

et al. 2005

Planta

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“…Pectins are polysaccharidic polyelectrolytes whose degree of hydration depends on the equilibrium between neutral carboxylic residues (e.g. due to methyl esterification) and exposed negative charges of dissociated carboxyls (Dähnert and Huster, 1999;Ryden et al, 2000). Cations interfere with this equilibrium causing pectins to shrink (Willats et al, 2001a) with consequent increase in the dimensions of pores in the pit membranes and, hence, in K s .…”

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confidence: 99%

Reduced Content of Homogalacturonan Does Not Alter the Ion-Mediated Increase in Xylem Hydraulic Conductivity in Tobacco

Nardini¹,

Gascó²,

Cervone³

et al. 2007

Plant Physiology

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Xylem hydraulic conductivity (K s ) in stems of tobacco (Nicotiana tabacum) wild-type SR1 was compared to that of PG7 and PG16, two transgenic lines with increased levels of expression of the gene encoding the Aspergillus niger endopolygalacturonase (AnPGII). Activity of AnPGII removes in planta blocks of homogalacturonan (HG) with deesterified carboxyls, thus increasing the degree of neutrality of pectins. The effect of K 1 was tested in increasing stem K s using model plants with more neutral polysaccharides in primary walls and, hence, in intervessel pit membranes. K s measured with deionized water was compared to that with KCl solutions at increasing concentrations (DK s , %). Plants transformed for HG degree of neutrality showed a dwarfed phenotype, but DK s did not differ among the three experimental groups. The ion-mediated hydraulic effect saturated at a KCl concentration of 25 mM in SR1 plants. All the three tobacco lines showed DK s of around 112.5% and 117.0% when perfused with 10 and 25 mM KCl, respectively. Because modification of HG content did not influence ion-mediated hydraulic enhancement, we suggest that pectin components other than HG, like rhamnogalacturonan-I and/or rhamnogalacturonan-II, might play important roles in the hydrogel behavior of pit membranes.

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“…2) -potato-pulp shows excellent adhesive properties (Mayer 1998). Up to 17% of the dry matter of the potato tissue is pectin (Mayer & Hillebrandt 1997) which has good water solubility and a high ability of swelling (Jarvis 1984, Ryden et al 2000, Zsivanovits et al 2004.…”

Section: Mechanically-enzymatically Decomposed Potato-pulp In Productmentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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Hydration of pectic polysaccharides

Cited by 71 publications

References 37 publications

Distribution of pectic epitopes in cell walls of the sugar beet root

Distribution of pectic epitopes in cell walls of the sugar beet root

Reduced Content of Homogalacturonan Does Not Alter the Ion-Mediated Increase in Xylem Hydraulic Conductivity in Tobacco

Wood production, wood technology, and biotechnological impacts

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