Hydrocolloid Coating of Xenopus laevis Embryos

Kampf, Nir; Zohar, C.; Nussinovitch, A.

doi:10.1021/bp0000252

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…For instance, agarose, carrageenan or low-methoxy pectin biopolymers have been used to generate a 50-mm thick gel veneer around newly fertilized toad eggs. 102,103 This process is an excellent example of a single-cell coating (rather than gel encapsulation) and was shown to be effective at preventing microbial infection and improving posthatching survival rates. This study, however, was demonstrated using relatively large cells (diameter ¼ 1-1.5 mm) and has not been applied to smaller cells (diameter <100 mm).…”

Section: Extended Cellular Coatingsmentioning

confidence: 99%

Strategies for cell membrane functionalization

Armstrong

Perriman

2016

Exp Biol Med (Maywood)

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The ability to rationally manipulate and augment the cytoplasmic membrane can be used to overcome many of the challenges faced by conventional cellular therapies and provide innovative opportunities when combined with new biotechnologies. The focus of this review is on emerging strategies used in cell functionalization, highlighting both pioneering approaches and recent developments. These will be discussed within the context of future directions in this rapidly evolving field.

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Section: Extended Cellular Coatingsmentioning

confidence: 99%

Strategies for cell membrane functionalization

Armstrong

Perriman

2016

Exp Biol Med (Maywood)

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Hydrocolloid Coatings

2003

Water‐Soluble Polymer Applications in Foods

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The use of polymers for coating of cells

Kampf

2002

Polymers for Advanced Techs

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Bare cells or tissues are sometimes exposed to external hazardous conditions, physical (thermal changes), chemical or biological (such as enzymes, bacteria and viruses). Liquids, semi‐liquids (e.g. gels) or solid films are often used to surround individual cells or tissues in order to protect them and to achieve greater stability in laboratory and industrial uses. On the one hand there is a need for isolation and creating a barrier between the cell and its surroundings, and on the other hand this should not affect the metabolism and viability of the cell used. Polymers are major substances for cell encapsulation and entrapment. These preparations are often used for the manufacture of drugs and other cell metabolites. There are only a few publications describing single‐cell coating. We can consider a definition of coating as a process of surrounding an object with a thin film, having a thickness of only a small fraction of the object's dimensions. Taking this definition into account, in fact, most coating experiments actually deal with entrapment or immobilization rather than coating. The latest research shows for the first time the ability to coat a single cell by producing a thin gel layer around it. Xenopus laevis eggs and embryos were used as a model system for coating with thin films composed of low‐methoxy pectin (LMP), alginate, and ι‐ and κ‐carrageenans. These gums have different compositions and structures and accordingly created different coatings around the cells. The first goal was to achieve a thin film (∼50 µm) around an individual non‐fertilized egg (one cell). Next, the influence of coating an early‐stage toad embryo was tested. All coated embryos appeared to develop normally, similar to non‐coated embryos. The survival percentages after hatching of coated embryos were significantly higher than of the non‐coated control. In addition, the coating served as a barrier to microbial contamination and thus improved survival prospects. The coatings delayed hatching by 18 to 24 hr. At hatch, the embryos were at a more developed stage than their non‐coated counterparts. Thus, coating cells by polymeric thin film appears to be a suitable tool for laboratories interested in performing longer‐term experiments with cells or embryos. Copyright © 2003 John Wiley & Sons, Ltd.

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Hydrocolloid Coating of Xenopus laevis Embryos

Cited by 4 publications

References 29 publications

Strategies for cell membrane functionalization

Strategies for cell membrane functionalization

Hydrocolloid Coatings

The use of polymers for coating of cells

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