Hydrodynamic Chromatography of Waxy Maize Starch

Abstract

A hydrodynamic column packed with solid beads chemically bonded with N‐methyl‐d‐glucamine residues was used with 90% dimethylsulfoxide (DMSO)‐H2O mobile phase as part of a chromatographic system to characterize jet‐cooked waxy maize starch. Software calculations based on signals from refractive index and dual‐angle light‐scattering detectors indicated the column could fractionate molecular weights up to ≈5 × 108. Calculated molecular weight values for the highest molecular weight sample was greatest at the lowest flow rate of 0.1 mL/min. Values of molecular weights and radii of gyration determined by the in‐line dual‐angle light‐scattering detector were significantly less than those determined with an off‐line multiangle light‐scattering detector that examined samples that had not traveled through the hydrodynamic column. This work has demonstrated the feasibility of using a hydrodynamic column to characterize waxy maize amylopectins. However, considerations of sample shear sensitivity and questions of in‐line light scattering detection show that further efforts are required to develop and optimize a chromatographic system to characterize very high molecular weight amylopectins.

Molecular Distribution and Pasting Properties of UV-Irradiated Corn Starches

“…On the other hand, Hanselmann et al [12] reported a molecular weight of 360 × 10 6 for waxy maize starch obtained from sedimentation field flow fraction. The molecular weight of jet-cooked waxy maize starch measured by Klavons et al [13], was found to be 412 × 10 6 in batch-mode, but 224 × 10 6 by hydrodynamic columns. Aberle et al [14] measured M w and R g of amylopectin and amylose fractions of several starches in very diluted aqueous solutions by static laser light scattering experiments.…”

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

“…HDC can be used in the size fractionation of organic and water soluble synthetic polymers [4-13, 52, 92, 93] or biopolymers [5,9,11,[17][18][19][20]52]. Prud´homme et al [93] developed a method for characterizing the molecular weight and molecular weight distribution of ultra-high molecular weight water soluble polymers (polyacrylamide, poly-acrylamide/acrylic acid copolymers, etc.).…”

“…Meanwhile, it was found application for HDC in a broad range of separations such as polymer latexes [2,3], rigid and flexible polymers [4][5][6][7][8][9][10][11][12][13], silica particles [14], zeolites [15], viruses [5], bacteria and yeasts [16], proteins [17], plasmid DNAs [17,18], starch [19,20], gold colloids [21], liposomes [21] and nanocapsules [22], these separations being performed using columns packed with nonporous particles (packed-column hydrodynamic chromatography (PCHDC)) or in open capillaries (capillary hydrodynamic chromatography (CHDC)).…”

Friction properties of chemically modified starch

“…The estimated molecular weight of a linear starch biopolymer amylose is in the range of 0.5-2.0 × 10 6 [7]. The molecular weight of amylopectin is several orders of magnitude higher than that of amylose starch [1,8].…”