Hydrodynamic control of titania nanotube formation on Ti-6Al-4V alloys enhances osteogenic differentiation of human mesenchymal stromal cells

Li, Jun; Mutreja, Isha; Tredinnick, Seamus; Jermy, Mark; Hooper, G.; Woodfield, Tim B. F.

doi:10.1016/j.msec.2019.110562

Cited by 26 publications

(18 citation statements)

References 57 publications

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“…Polishing techniques [4,5], or laser treatments [6] may be used to enhance the roughness and the hydrophilicity of the metallic substrate, that are known to induce better cell adhesion. Anodization of the surface to form titanium oxide nanotubes is also developed [7][8][9], as TiO 2 nanotubes enhance cell growth. Another approach is to coat the metallic surface with calcium phosphate material, due to its similarity with bone.…”

Section: Introductionmentioning

confidence: 99%

Baicalein-modified hydroxyapatite nanoparticles and coatings with antibacterial and antioxidant properties

Palierse

Hélary

Krafft

et al. 2021

Materials Science and Engineering: C

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Aseptic loosening and bacterial infections are the two main causes of failure for metallic implants used for joint replacement. A coating that is both bioactive and possesses antimicrobial properties may address such shortcomings and improve the performance of the implant. We have sought to study the properties of combining hydroxyapatite-based nanoparticles or coatings with baicalein, a plant-extracted molecule with both antibacterial and antioxidant properties. (B-type) carbonated hydroxyapatite nanoparticles prepared by a chemical wet method could subsequently adsorbed by soaking in a baicalein solution. The amount of adsorbed baicalein was determined to be 63 mg.g -1 by thermogravimetric measurements. In a second approach, baicalein was adsorbed on a biomimetic calciumdeficient hydroxyapatite planar coating (12 m thick) deposited on Ti6Al4V alloy from an aqueous solution of calcium, phosphate, sodium and magnesium salts. Soaking of the hydroxyapatite coated on titanium alloy in a baicalein solution induced partial dissolution/remodeling of the upper surface of the coating. However, the observed remodeling of the surface was much more pronounced in the presence of a baicalein solution, compared to pure water. The presence of adsorbed baicalein on the HAp layer, although it could not be precisely quantified, was assessed by XPS and fluorescence analysis. Planar coatings exhibited significant antibacterial properties against Staphylococcus epidermidis. Baicaleinmodified nanoparticles exhibited significant antioxidant properties. These results illustrate the potential of hydroxyapatite used as a carrier for natural biologically-active molecules and also discuss the challenges associated with their applications as antibacterial agents.

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Section: Introductionmentioning

confidence: 99%

Baicalein-modified hydroxyapatite nanoparticles and coatings with antibacterial and antioxidant properties

Palierse

Hélary

Krafft

et al. 2021

Materials Science and Engineering: C

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“…Our previous work based on anodization of Ti‐6Al‐4V and other publications demonstrated a reduction in Ti, Al, and V content compared with polished control, whereas O and F were detected on anodized Ti‐6Al‐4V surfaces. [ 27,71,72 ] However, F content was not expected to influence biocompatibility for application of anodized surfaces since in vitro and in vivo studies have validated the supportive effect on bone regeneration of anodized surfaces. [ 72,73 ] Furthermore, fluoride‐modified dental implants via electrochemical etching illustrated the significant improvement of bone regeneration and has been widely used in clinical applications, even though it is difficult to distinguish the individual contribution made by surface texture or chemical composition.…”

Section: Resultsmentioning

confidence: 99%

“…The nanostructures are known to enhance osteogenesis as demonstrated in our previous study and can also be used as reservoirs for controlled drug delivery. [ 21–26,27 ] For combating infection, one of the attractive options is silver ions that have demonstrated a broad antimicrobial spectrum. [ 28,29 ] However, the toxicity of silver to eukaryotic cells, along with compromised osseointegration and inflammatory response, has been a concern.…”

Section: Introductionmentioning

confidence: 99%

Combined Infection Control and Enhanced Osteogenic Differentiation Capacity on Additive Manufactured Ti‐6Al‐4V are Mediated via Titania Nanotube Delivery of Novel Biofilm Inhibitors

Mutreja

Hooper

et al. 2020

Adv Materials Inter

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Additive manufacturing (AM) of titanium alloys offers the capacity to fabricate patient-specific implants with defined porous architecture to enhance bone-implant fixation. However, clinical challenges associated with orthopedic implants include inconsistent osseointegration and biofilm-associated peri-implant infection, leading to implant failure. Here, a strategy is developed to reduce infection and promote osteogenesis simultaneously on AM Ti-6Al-4V implants by delivering biofilm inhibitor molecules via titania nanotube surface modification. Electrochemical anodization is performed on polished and as-manufactured Ti-6Al-4V to generate nanotubes, which are utilized for delivery of a novel methylthioadenosine nucleosidase inhibitor (MTANi) that targets MTAN-a key enzyme in bacterial metabolism involved in biofilm formation-thereby offering biofilm inhibition capacity combined with surface nano-topography for promoting osteogenesis. Clinical isolates of staphylococcus cohnii formed firm biofilms on polished and AM Ti-6Al-4V controls whereas modified implants loaded with MTANi inhibit biofilm formation. Anodized AM Ti-6Al-4V nanotube substrates enhance alkaline phosphatase production, bone-specific protein expression (osteocalcin, collagen I) and mineral deposition of human mesenchymal stromal cells (hMSCs), compared to as-manufactured controls. Importantly, no detrimental effects on hMSC proliferation and osteogenic differentiation are observed for MTANi-loaded substrates. Application of novel MTANi and electrochemical anodization offers a promising strategy for titanium alloy implant surface modification.

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“…They then evaluated the osteogenic differentiation capacity of human MSCs on their surfaces by further cell seeding and culture assays. The results showed a significant increase in the expression of osteocalcin and osteopontin as well as mineralization deposition in the 39 nm group [ 192 ]. In another example, 3D-printed porous Ti biomaterial covered with nano-tubular surfaces was developed using an optimized anodizing protocol, which was demonstrated to prevent biofilm formation when loaded with middle and high concentrations of Ag ions.…”

Section: Nano-medicine In Bone Repairmentioning

confidence: 99%

The advances in nanomedicine for bone and cartilage repair

Qiao

Tang

et al. 2022

J Nanobiotechnol

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With the gradual demographic shift toward an aging and obese society, an increasing number of patients are suffering from bone and cartilage injuries. However, conventional therapies are hindered by the defects of materials, failing to adequately stimulate the necessary cellular response to promote sufficient cartilage regeneration, bone remodeling and osseointegration. In recent years, the rapid development of nanomedicine has initiated a revolution in orthopedics, especially in tissue engineering and regenerative medicine, due to their capacity to effectively stimulate cellular responses on a nanoscale with enhanced drug loading efficiency, targeted capability, increased mechanical properties and improved uptake rate, resulting in an improved therapeutic effect. Therefore, a comprehensive review of advancements in nanomedicine for bone and cartilage diseases is timely and beneficial. This review firstly summarized the wide range of existing nanotechnology applications in the medical field. The progressive development of nano delivery systems in nanomedicine, including nanoparticles and biomimetic techniques, which are lacking in the current literature, is further described. More importantly, we also highlighted the research advancements of nanomedicine in bone and cartilage repair using the latest preclinical and clinical examples, and further discussed the research directions of nano-therapies in future clinical practice. Graphical Abstract

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Hydrodynamic control of titania nanotube formation on Ti-6Al-4V alloys enhances osteogenic differentiation of human mesenchymal stromal cells

Cited by 26 publications

References 57 publications

Baicalein-modified hydroxyapatite nanoparticles and coatings with antibacterial and antioxidant properties

Baicalein-modified hydroxyapatite nanoparticles and coatings with antibacterial and antioxidant properties

Combined Infection Control and Enhanced Osteogenic Differentiation Capacity on Additive Manufactured Ti‐6Al‐4V are Mediated via Titania Nanotube Delivery of Novel Biofilm Inhibitors

The advances in nanomedicine for bone and cartilage repair

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