Hydrodynamic Flow Confinement Technology in Microfluidic Perfusion Devices

Ainla, Alar; Jeffries, Gavin D. M.; Jesorka, Aldo

doi:10.3390/mi3020442

Cited by 20 publications

(11 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, one of the drawbacks of “closed” microfluidics is the challenge to process objects likely to induce clogging or that are simply too large to fit in a channel network, such as tissue sections, large cells and particles. Open microfluidic systems overcome some of these problems by using hydrodynamic flow confinement (HFC) to confine a fluid stream such as in microfluidic probes (MFP) 4 5 6 or capillary effects to confine droplets between a probe tip and a surface, such as in the “chemistrode” configuration 10 7 8 . Using the HFC property of MFPs, reagents injected by the probe can be confined within close proximity of the probe, allowing their delivery to a surface with high spatial resolution and lower shear stress than in channel-based microfluidics systems.…”

mentioning

confidence: 99%

Two-Aperture Microfluidic Probes as Flow Dipoles: Theory and Applications

Safavieh

Qasaimeh

Vakil

et al. 2015

Sci Rep

View full text Add to dashboard Cite

A microfluidic probe (MFP) is a mobile channel-less microfluidic system under which a fluid is injected from an aperture into an open space, hydrodynamically confined by a surrounding fluid, and entirely re-aspirated into a second aperture. Various MFPs have been developed, and have been used for applications ranging from surface patterning of photoresists to local perfusion of organotypic tissue slices. However, the hydrodynamic and mass transfer properties of the flow under the MFP have not been analyzed, and the flow parameters are adjusted empirically. Here, we present an analytical model describing the key transport properties in MFP operation, including the dimensions of the hydrodynamic flow confinement (HFC) area, diffusion broadening, and shear stress as a function of: (i) probe geometry (ii) aspiration-to-injection flow rate ratio (iii) gap between MFP and substrate and (iv) reagent diffusivity. Analytical results and scaling laws were validated against numerical simulations and experimental results from published data. These results will be useful to guide future MFP design and operation, notably to control the MFP “brush stroke” while preserving shear-sensitive cells and tissues.

show abstract

mentioning

confidence: 99%

Two-Aperture Microfluidic Probes as Flow Dipoles: Theory and Applications

Safavieh

Qasaimeh

Vakil

et al. 2015

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Small dimensions of the microchannel may raise the risk of clogging by big particles or cells, clumps of cells or even extraneous debris. Details about basic concepts, fabrication strategies and advanced applications of hydrodynamic focusing in microflow cytometers can be found in a review by Ainla et al [ 23 ].…”

Section: Major Components Of An Optofluidic Microflow Cytometermentioning

confidence: 99%

Optofluidic Device Based Microflow Cytometers for Particle/Cell Detection: A Review

et al. 2016

View full text Add to dashboard Cite

Optofluidic devices combining micro-optical and microfluidic components bring a host of new advantages to conventional microfluidic devices. Aspects, such as optical beam shaping, can be integrated on-chip and provide high-sensitivity and built-in optical alignment. Optofluidic microflow cytometers have been demonstrated in applications, such as point-of-care diagnostics, cellular immunophenotyping, rare cell analysis, genomics and analytical chemistry. Flow control, light guiding and collecting, data collection and data analysis are the four main techniques attributed to the performance of the optofluidic microflow cytometer. Each of the four areas is discussed in detail to show the basic principles and recent developments. 3D microfabrication techniques are discussed in their use to make these novel microfluidic devices, and the integration of the whole system takes advantage of the miniaturization of each sub-system. The combination of these different techniques is a spur to the development of microflow cytometers, and results show the performance of many types of microflow cytometers developed recently.

show abstract

“…A PCD with the edge pixels having a negative net flow rate, such as in Fig. 2 A-C, operates in the well-established hydrodynamic flow confinement regime (12,20). For any aperture configuration other than microfluidic pixels, including every previously published open-space microfluidic system, this regime is the only possible way to confine streaming reagents since, without a net aspiration from the device, they would diffuse to infinity and escape confinement.…”

Section: Significancementioning

confidence: 99%

“…For example, the scanning speed of reagent-streaming devices is limited by the incubation time of the reagents involved in the surface reaction, often minutes to hours for protein-protein reactions (immunoassays) or cell stimulation (e.g., drug incubation). Finally, all open-space microfluidic systems developed so far can only operate on immersed surfaces since, for fluid confinement to occur, more fluid must be aspirated from the probe than what is injected, with the excess taken from the immersion medium (12)(13)(14).…”

mentioning

confidence: 99%

Pixel-based open-space microfluidics for versatile surface processing

Goyette

Boulais

Tremblay

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

An increasing number of applications in biology, chemistry, and material sciences require fluid manipulation beyond what is possible with current automated pipette handlers, such as gradient generation, interface reactions, reagent streaming, and reconfigurability. In this article, we introduce the pixelated chemical display (PCD), a scalable strategy for highly parallel, reconfigurable liquid handling on open surfaces. Microfluidic “pixels” are created when a fluid stream injected above a surface is confined by neighboring identical fluid streams, forming a repeatable flow unit that can be used to tesselate a surface. PCDs generating up to 144 pixels are fabricated and used to project “chemical moving pictures” made of several reagents over both immersed and dry surfaces, without any physical barrier or wall. This work distinguishes itself from previous work in open-space microfluidics by presenting a device architecture where the number of confinement areas can be scaled to any size. Furthermore, it challenges the open-space tenet that the aspiration rate must be higher than the injection rate for reagents to be confined. Overall, this article sets the foundation for massively parallel surface processing using continuous flow streams and showcases possibilities in both wet and dry surface patterning and roll-to-roll processes.

show abstract

Hydrodynamic Flow Confinement Technology in Microfluidic Perfusion Devices

Cited by 20 publications

References 39 publications

Two-Aperture Microfluidic Probes as Flow Dipoles: Theory and Applications

Two-Aperture Microfluidic Probes as Flow Dipoles: Theory and Applications

Optofluidic Device Based Microflow Cytometers for Particle/Cell Detection: A Review

Pixel-based open-space microfluidics for versatile surface processing

Contact Info

Product

Resources

About