Hydrodynamic Stress and Heterogeneities in Animal Cell Culture

Palomares, Laura A.; Ramı́rez, Octavio T.

doi:10.1016/b978-0-444-64046-8.00139-7

Cited by 1 publication

(1 citation statement)

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“…Bioreactors are the backbone of countless biological production processes for high‐value products in the pharmaceutical and biotechnological industry by enabling the efficient cultivation of bacteria, fungi, and animal cells. However, despite technological advances in sensor technology, process control, and vessel material, the literature (Mandenius, 2016; Palomares & Ramírez, 2019) highlights that aeration as one of the major bioreactor design criteria has experienced little innovation since the twentieth century: The gas supply in submerged cultures is still performed by bubble dispersion and stirring. Due to this lack of innovation, conventional bioreactor types cannot combine low‐stress mixing conditions with high gas entry.…”

Section: Introductionmentioning

confidence: 99%

A novel membrane stirrer system enables foam‐free biosurfactant production

Bongartz

Karmainski

Meyer

et al. 2023

Biotech & Bioengineering

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Bioreactors are the operative backbone, for example, for the production of biopharmaceuticals, biomaterials in tissue engineering, and sustainable substitutes for chemicals. Still, the Achilles' heel of bioreactors nowadays is the aeration which is based on intense stirring and gas sparging, yielding inherent drawbacks such as shear stress, foaming, and sterility concerns. We present the synergistic combination of simulations and experiments toward a membrane stirrer for the efficient bubble-free aeration of bioreactors. A digital twin of the bioreactor with an integrated membrane-module stirrer (MemStir) was developed with computational fluid dynamics (CFD) studies addressing the determination of fluid mixing, shear rates, and local oxygen concentration.Usability of the MemStir is shown in a foam-free recombinant production process of biosurfactants (rhamnolipids) from glucose with different strains of Pseudomonas putida KT2440 in a 3-L vessel and benchmarked against a regular aerated process. The MemStir delivered a maximal oxygen transfer rate (OTR max ) of 175 mmol L −1 h −1 in completely foam-free cultivations. With a high space-time yield (STY) of 118 mg RL L −1 h −1 during a fed-batch fermentation, the effectiveness of the novel MemStir is demonstrated. Simulations show the generic value of the MemStir beyond biosurfactant production, for example, for animal cell cultivation.

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Section: Introductionmentioning

confidence: 99%