Hydrogel-Based Platforms for the Regeneration of Osteochondral Tissue and Intervertebral Disc

Guarino, Vincenzo; Gloria, Antonio; Raucci, Maria Grazia; Ambrosio, Luigi

doi:10.3390/polym4031590

Cited by 58 publications

(38 citation statements)

References 144 publications

(185 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These scaffolds fabricated using optimum phase separation parameters thus improve the medium transport capabilities and protein adsorption abilities, and may be potentially useful for tissue engineering applications by absorbing larger amount of proteins and growth factors. 44 Further experiments will be carried out to determine the ability of the porous scaffold to support cell attachment, proliferation, and differentiation in vitro .…”

Section: Resultsmentioning

confidence: 99%

Novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) porous scaffolds fabricated by phase separation for tissue engineering applications

et al. 2015

View full text Add to dashboard Cite

Scaffolds with intrinsically interconnected porous structures are highly desirable in tissue engineering and regenerative medicine. In this study, three-dimensional polymer scaffolds with highly interconnected porous structures were fabricated by thermally induced phase separation of novel synthesized biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) in a dioxane/water binary system. Defined porous scaffolds were achieved by optimizing conditions to attain interconnected porous structures. The effect of phase separation parameters on scaffold morphology were investigated, including polymer concentration (1, 3, 5, 7, and 9%), quench time (1, 4, and 8 min), dioxane/water ratio (83/17, 85/15, and 87/13 wt/wt), and freeze temperature (−20, −80, and −196 °C). Interesting pore morphologies were created by adjusting these processing parameters, e.g., flower-shaped (5%; 85/15; 1 min; −80 °C), spherulite-like (5%; 85/15; 8 min; −80 °C), and bead-like (5%; 87/13; 1 min; −80 °C) morphology. Modulation of phase separation conditions also resulted in remarkable differences in scaffold porosities (81% to 91%) and thermal properties. Furthermore, scaffolds with varied mechanic strengths, degradation rates, and protein adsorption capabilities could be fabricated using the phase separation method. In summary, this work provides an effective route to generate multi-dimensional porous scaffolds that can be applied to a variety of hydrophobic polymers and copolymers. The generated scaffolds could potentially be useful for various tissue engineering applications including bone tissue engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

Novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) porous scaffolds fabricated by phase separation for tissue engineering applications

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This peculiar capability, to generate a highly hydrated microenvironment, also allows for protecting sensitive drugs, thus preserving molecular stability prior to the delivery at the site of injury [4]. Moreover, this assures an efficient transport of biological substances, such as nutrients and products from cell metabolism, in and out of the hydrogels [5], which are fundamental to protect and sustain cell viability during the regeneration processes [6].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Microgels and Nanoparticles via Electrofluidodynamic Techniques for Biomedical Applications

Guarino

Altobelli

Ambrosio

2016

Gels

Self Cite

View full text Add to dashboard Cite

Electrofluidodynamics techniques (EFDTs) are emerging methodologies based on liquid atomization induced by electrical forces to obtain a fine suspension of particles from hundreds of micrometers down to nanometer size. As a function of the characteristic size, these particles are interesting for a wide variety of applications, due to the high scalability of chemical and physical properties in comparison to the bulk form. Here, we propose the optimization of EFDT techniques to design chitosan systems in the form of microgels or nanoparticles for several biomedical applications. Different microscopy techniques (Optical, SEM, TEM) have been used to investigate the morphology of chitosan systems at multiple size scale. The proposed study confirms the high versatility and feasibility of EFDTs for creating micro and nano-sized carriers for cells and drug species.

show abstract

“…In particular, in situ forming polymeric hydrogel materials have been gaining recent popularity in the field of osteochondral tissue regeneration [19, 20]. As part of this effort, our laboratory has developed a novel class of water soluble oligo(poly(ethylene glycol) fumarate) (OPF) macromers that can be chemically crosslinked to yield hydrolytically degradable and injectable hydrogels [21, 22].…”

Section: Introductionmentioning

confidence: 99%

Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels

Lam

Meretoja

et al. 2014

Acta Biomaterialia

View full text Add to dashboard Cite

This study investigated the capacity of chondrogenic and osteogenic pre-differentiation of mesenchymal stem cells (MSCs) for the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites. We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors. During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression. Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups. Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycans-to-hydroxyproline ratios. Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells. Groups with osteogenic cells displayed mineralization in the subchondral layer, confirming the effect of osteogenic pre-differentiation. In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic pre-differentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control. Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.

show abstract

Hydrogel-Based Platforms for the Regeneration of Osteochondral Tissue and Intervertebral Disc

Cited by 58 publications

References 144 publications

Novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) porous scaffolds fabricated by phase separation for tissue engineering applications

Novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) porous scaffolds fabricated by phase separation for tissue engineering applications

Chitosan Microgels and Nanoparticles via Electrofluidodynamic Techniques for Biomedical Applications

Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels

Contact Info

Product

Resources

About