Hydrogel composite scaffolds with an attenuated immunogenicity component for bone tissue engineering applications

Gao, Chenyuan; Sow, Wan Ting; Wang, Yingying; Wang, Yili; Yang, Dejun; Lee, Bae Hoon; Matičić, Dražen; Lian, Fang; Li, Huaqiong; Zhang, Chunwu

doi:10.1039/d0tb02588g

Cited by 29 publications

(28 citation statements)

References 32 publications

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“…The incorporation of BP into the GelMA matrix decreases the hydrophilic GelMA component within these scaffolds [ 9 , 10 , 25 ]. Due to the reduced matrix hydrophilicity, there is less water retention within the GelMA/BP scaffolds and, as a result, they swell less compared to the GelMA scaffolds with no BP.…”

Section: Resultsmentioning

confidence: 99%

Composite Scaffolds from Gelatin and Bone Meal Powder for Tissue Engineering

Lantigua

Suvarnapathaki

et al. 2021

Bioengineering

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Bone tissue engineering offers versatile solutions to broaden clinical options for treating skeletal injuries. However, the variety of robust bone implants and substitutes remains largely uninvestigated. The advancements in hydrogel scaffolds composed of natural polymeric materials and osteoinductive microparticles have shown to be promising solutions in this field. In this study, gelatin methacrylate (GelMA) hydrogels containing bone meal powder (BP) particles were investigated for their osteoinductive capacity. As natural source of the bone mineral, we expect that BP improves the scaffold’s ability to induce mineralization. We characterized the physical properties of GelMA hydrogels containing various BP concentrations (0, 0.5, 5, and 50 mg/mL). The in vitro cellular studies revealed enhanced mechanical performance and the potential to promote the differentiation of pre-osteoblast cells. The in vivo studies demonstrated both promising biocompatibility and biodegradation properties. Overall, the biological and physical properties of this biomaterial is tunable based on BP concentration in GelMA scaffolds. The findings of this study offer a new composite scaffold for bone tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Composite Scaffolds from Gelatin and Bone Meal Powder for Tissue Engineering

Lantigua

Suvarnapathaki

et al. 2021

Bioengineering

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“…In in vitro studies, dECM powder can very effectively promote cell attachment and proliferation [ 13 ]. Thus, human dECM powder can be used as efficient injection biomaterials for tissue engineering with great potential in addressing the clinical challenges of periodontal regenerative medicine [ 8 , 70 , 71 ]. In addition, decellularized bone powder, as a commonly used periodontal tissue engineering material, is another form of clinical application of dECM granules, which are often combined with hydrogel and a guiding tissue regeneration membrane to promote periodontal regeneration [ 50 , 71 , 72 ].…”

Section: Application Of Decm Of Different Forms In Periodontal Regene...mentioning

confidence: 99%

Advances Focusing on the Application of Decellularized Extracellular Matrix in Periodontal Regeneration

2023

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The decellularized extracellular matrix (dECM) is capable of promoting stem cell proliferation, migration, adhesion, and differentiation. It is a promising biomaterial for application and clinical translation in the field of periodontal tissue engineering as it most effectively preserves the complex array of ECM components as they are in native tissue, providing ideal cues for regeneration and repair of damaged periodontal tissue. dECMs of different origins have different advantages and characteristics in promoting the regeneration of periodontal tissue. dECM can be used directly or dissolved in liquid for better flowability. Multiple ways were developed to improve the mechanical strength of dECM, such as functionalized scaffolds with cells that harvest scaffold-supported dECM through decellularization or crosslinked soluble dECM that can form injectable hydrogels for periodontal tissue repair. dECM has found recent success in many periodontal regeneration and repair therapies. This review focuses on the repairing effect of dECM in periodontal tissue engineering, with variations in cell/tissue sources, and specifically discusses the future trend of periodontal regeneration and the future role of soluble dECM in entire periodontal tissue regeneration.

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“…In addition, with the development of genetic engineering technology, it is anticipated that implantable liver tissue constructs will be fabricated; these can subsequently be applied in clinical treatments with dECM prepared from xenogeneic or allogeneic tissues, wherein the immunogenic genes will be removed. To support this, a research team utilized porcine tissue as a material for dECM to print bone tissues, wherein the immune-rejection gene, α-1,3-galactose (α-1,3-gal), was knocked out ( Gao et al, 2021 ).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

Kim

Lee

et al. 2022

Front. Bioeng. Biotechnol.

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Three-dimensional (3D)-printed in vitro tissue models have been used in various biomedical fields owing to numerous advantages such as enhancements in cell response and functionality. In liver tissue engineering, several studies have been reported using 3D-printed liver tissue models with improved cellular responses and functions in drug screening, liver disease, and liver regenerative medicine. However, the application of conventional single-component bioinks for the printing of 3D in vitro liver constructs remains problematic because of the complex structural and physiological characteristics of the liver. The use of multicomponent bioinks has become an attractive strategy for bioprinting 3D functional in vitro liver tissue models because of the various advantages of multicomponent bioinks, such as improved mechanical properties of the printed tissue construct and cell functionality. Therefore, it is essential to review various 3D bioprinting techniques and multicomponent hydrogel bioinks proposed for liver tissue engineering to suggest future directions for liver tissue engineering. Accordingly, we herein review multicomponent bioinks for 3D-bioprinted liver tissues. We first describe the fabrication methods capable of printing multicomponent bioinks and introduce considerations for bioprinting. We subsequently categorize and evaluate the materials typically utilized for multicomponent bioinks based on their characteristics. In addition, we also review recent studies for the application of multicomponent bioinks to fabricate in vitro liver tissue models. Finally, we discuss the limitations of current studies and emphasize aspects that must be resolved to enhance the future applicability of such bioinks.

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Hydrogel composite scaffolds with an attenuated immunogenicity component for bone tissue engineering applications

Abstract: Xenogeneic bones are potential templates for bone regeneration. In this study, decellularized porcine bone powder with attenuated immunogenicity was incorporated into a photocurable hydrogel, gelatin methacryloyl (GelMA), to obtain scaffolds...

Cited by 29 publications

References 32 publications

Composite Scaffolds from Gelatin and Bone Meal Powder for Tissue Engineering

Composite Scaffolds from Gelatin and Bone Meal Powder for Tissue Engineering

Advances Focusing on the Application of Decellularized Extracellular Matrix in Periodontal Regeneration

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

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