Hydrogel platform capable of molecularly resolved pulling on cells for mechanotransduction

Chen, Nan; Zhang, Yile; Wu, Yukai; Li, Bohan; Wang, Hong; Chen, Shaojie; Zhao, Peng; Cui, Jiaxi; Shen, Xiaoqin; Zhu, Xingjun; Zheng, Yijun

doi:10.1016/j.mtbio.2022.100476

Cited by 3 publications

(6 citation statements)

References 70 publications

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“…While it has been hypothesized that the reversible nature of DCCs would invariably lead to injectable hydrogels, [13,[16][17][18] that is not always the case. DCC hydrogels present a range of extrudability behavior, with many gel strategies that are too difficult to extrude by hand [21,35,61,62] or extrude as fragmented gels that cannot regain their original mechanical properties. [35,[63][64][65][66][67] There-fore, further exploration is necessary to uncover the fundamental design rules of dynamic polymer networks that would lead to hydrogels with both ease of injectability and robust mechanics.…”

Section: Click-chemistry Allows For Controlled Functionalization Of H...mentioning

confidence: 99%

Design Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks

Narciso

Gilchrist

Trigo

et al. 2023

Adv Healthcare Materials

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Dynamic covalent chemistry (DCC) crosslinks can form hydrogels with tunable mechanical properties permissive to injectability and self‐healing. However, not all hydrogels with transient crosslinks are easily extrudable. For this reason, two additional design parameters must be considered when formulating DCC‐crosslinked hydrogels: 1) degree of functionalization (DoF) and 2) polymer molecular weight (MW). To investigate these parameters, hydrogels comprised of two recombinant biopolymers: 1) a hyaluronic acid (HA) modified with benzaldehyde and 2) an elastin‐like protein (ELP) modified with hydrazine (ELP‐HYD), are formulated. Several hydrogel families are synthesized with distinct HA MW and DoF while keeping the ELP‐HYD component constant. The resulting hydrogels have a range of stiffnesses, G′ ≈ 10–1000 Pa, and extrudability, which is attributed to the combined effects of DCC crosslinks and polymer entanglements. In general, lower MW formulations require lower forces for injectability, regardless of stiffness. Higher DoF formulations exhibit more rapid self‐healing. Gel extrusion through a cannula (2 m length, 0.25 mm diameter) demonstrates the potential for minimally invasive delivery for future biomedical applications. In summary, this work highlights additional parameters that influence the injectability and network formation of DCC‐crosslinked hydrogels and aims to guide future design of injectable hydrogels.

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Section: Click-chemistry Allows For Controlled Functionalization Of H...mentioning

confidence: 99%

Design Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks

Narciso

Gilchrist

Trigo

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Then the sensors were rinsed with pH 5.5 Milli-Q water, causing an increase in frequency, indicating the removal of weakly attached polymers. After nanofilm assembly, the EDTA washing step markedly increased the frequency, indicating the washing away of copper ions remaining in the nanofilms. , …”

Section: Resultsmentioning

confidence: 99%

“…Upon alteration of different polymer solutions, LbL nanofilms could be fabricated via the click reaction until they were assembled to the desired number of layers. EDTA (0.02 M) solutions were used to wash the copper ions away by forming an EDTA–Cu complex. , …”

Section: Methodsmentioning

confidence: 99%

“…After nanofilm assembly, the EDTA washing step markedly increased the frequency, indicating the washing away of copper ions remaining in the nanofilms. 24,25 Theoretically, a higher pH reduces the number of electrostatic interactions between PLL chains. As shown in Figure S7, compared with the marked stepwise increase in thickness observed from the nanofilm assembled at pH 5.5, nanofilms could barely assemble at pH 3.5.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…EDTA (0.02 M) solutions were used to wash the copper ions away by forming an EDTA−Cu complex. 24,25 The polymer mass (Δm) that was deposited on the QCM sensor was calculated according to the Sauerbrey equation. For the QCM machine used here, a frequency change of 1 Hz corresponded to a mass change of 0.62 ng/cm 2 (eq 1).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Fabrication of Fully Positively Charged Layer-by-Layer Polyelectrolyte Nanofilms with pH-Dependent Swelling Properties

Zhang,

Zeng,

Matsusaki

2024

Langmuir

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Nanofilms fabricated by layer-by-layer (LbL) assembly from polyelectrolytes (PEs) are important materials for various applications. However, PE films cannot retain the charges along the polymer chains during fabrication, resulting in a low charge density. In this study, the preparation of LbL nanofilms with preserved positive charges via a controllable and efficient approach was achieved. To fabricate fully positively charged (FPC) LbL nanofilms, a polycation, poly-L-lysine, was partially grafted with azide and alkyne groups. Through coppercatalyzed azide−alkyne cycloaddition and the LbL procedure, nanofilms were fabricated with all of the individual layers covalently bonded, improving the pH stability of the nanofilms. Because the resulting nanofilms had a high charge density with positive charges both inside and on the surface, they showed unique pH-dependent swelling properties and adsorption of negatively charged molecules compared with those of traditional polyelectrolyte LbL nanofilms. This kind of FPC nanofilm has great potential for use in sensors, diagnostics, and filter nanomaterials in the biomedical and environmental fields.

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NIR‐II Absorption/Emission Dual Function Based 2D Targeted Nanotheranostics for Tunable Hydrogenothermal Therapy

Li,

Gao,

Wang

et al. 2024

Adv Healthcare Materials

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Photothermal therapy (PTT) is a promising approach for treating tumors that offers multiple advantages. Nevertheless, its practical use in clinical settings faces several limitations, such as suboptimal delivery efficiency, uneven heat distribution, and challenges in predicting optimal treatment duration. In addition, the localized hyperthermia generated by the PTT method to induce cell apoptosis can result in the production of excessive reactive oxygen species (ROS) and the release of inflammatory cytokines, which can pose a threat to the healthy tissues surrounding the tumor. To address the above challenges, we designed an integrated H2 delivery nanoplatform for multimodal imaging H2 thermal therapy. The combination of the second near‐infrared window (NIR‐II) fluorescence imaging (FL) agent (CQ4T) and the photothermal and photoacoustic (PA) properties of Ti3C2 (TC) enables real‐time monitoring of the tumor area and guides photothermal treatment. Simultaneously, due to the acid‐responsive H2 release characteristics of the nanoplatform, H2 can be utilized for synergistic photothermal therapy to eradicate tumor cells effectively. Significantly, acting as an antioxidant and anti‐inflammatory agent, Ti3C2‐BSA‐CQ4T‐H2 (TCBCH) protects peritumoral normal cells from damage. The proposed technique utilizing H2 gas for combination therapies and multimodal imaging integration exhibits prospects for effective and secure treatment of tumors in future clinical applications.This article is protected by copyright. All rights reserved

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Hydrogel platform capable of molecularly resolved pulling on cells for mechanotransduction

Cited by 3 publications

References 70 publications

Design Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks

Design Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks

Fabrication of Fully Positively Charged Layer-by-Layer Polyelectrolyte Nanofilms with pH-Dependent Swelling Properties

NIR‐II Absorption/Emission Dual Function Based 2D Targeted Nanotheranostics for Tunable Hydrogenothermal Therapy

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