Hydrogels for tissue engineering and regenerative medicine

Hunt, John A.; Chen, Rui; Veen, T. van der; Bryan, Nicholas

doi:10.1039/c4tb00775a

Cited by 325 publications

(200 citation statements)

References 216 publications

(138 reference statements)

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“…Hydrogels are an ideal tissue engineering material which can be sourced naturally, created synthetically or used in combination with other materials [61][62][63][64] . Hydrogel networks are comprised of polymer or peptide chains.…”

Section: Using Hydrogels For 3d Bioprintingmentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.

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Section: Using Hydrogels For 3d Bioprintingmentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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“…Hydrogels used in tissue engineering should have low viscosity before injection and should be gelling fast in the physiological environment of the tissue, and the most important is gelling (sol-gel transition) by cross-linking, which may take place when producing them in vitro and in vivo during injection. Physical cross-linking is used in particular in the case of poly(N-isopropylacrylamide) (poly(NIPAAM)), which may be used in tissue engineering after introducing acrylic acid (AAc) or PEG [460,461] or biodegradable polymers, including such as chitosan, gelation, hyaluronic acid and dextran [462][463][464][465][466] to block copolymers, such as poly(ethylene oxide) PEO-PPO-PEO (Pluronic), poly(lactide-co-glycolide) PLGA-PEG-PLGA, PEG-PLLA-PEG, polycaprolactone PCL-PEG-PCL and PEG-PCL-PEG [467][468][469][470][471], and also agarose (a polysaccharide polymer material, extracted from seaweed as one of the two principal components of agar) [459], as thermo-sensitive systems [472], to avoid the use of potentially cytotoxic ultraviolet radiation. Poly(NIPAAM) and block copolymer hydrogels may undergo cross-linking as a consequence of temperature and pH acting at the same time, as in the case of acrylates [473,474], such as 2-(dimethylamino)ethyl-methacrylate (DMAEMA) or 2-(diethylaminoethyl) methyl methacrylate.…”

Section: Selection Of Technologies Of Implantable Devices In Regeneramentioning

confidence: 99%

“…Ionic cross-linking hydrogels include calcium-cross-linked alginate [459] and chitosanpolylysine, chitosan-glycerol phosphate salt and chitosan-alginate hydrogels [506][507][508]. Different synthetic and natural polymers were used for this purpose, including polyethylene glycol (PEG), and copolymers containing PEG [486,510], hyaluronic acid (HA) [511] after an oxidation reaction through HA-tyramine conjugates [505] and as a result of the formation between HA-SH [492,512] and Michael addition [491,513], collagen and gelatin hydrogels mostly cross-linked using glutaraldehyde, genipin or water-soluble carbodiimides [513][514][515], chitosan [516][517][518][519], dextran 192 [520,521] and alginate [522].…”

Section: Selection Of Technologies Of Implantable Devices In Regeneramentioning

confidence: 99%

“…In the case of enzyme-mediated cross-linking [458], transglutaminases (including Factor Xllla) and horseradish peroxidases (HRP) [459] are used for the catalysis of star-shaped PEG hydrogels [496] and tissue transglutaminase catalysed PEG hydrogels [497]. This also applies tyrosinase, phosphopantetheinyl transferase, lysyl oxidase, plasma amine oxidase, and phosphatases [498].…”

Section: Selection Of Technologies Of Implantable Devices In Regeneramentioning

confidence: 99%

See 1 more Smart Citation

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

Dobrzański¹

2018

Biomaterials in Regenerative Medicine

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“…Due to the large water storing capability, hydrogels have been utilized in a myriad of applications, such as in agriculture, 136 contact lenses, 137 drug delivery devices, 138 sensors, 139 tissue engineering, 140 wound dressings, 141 cosmetic products, 142 regenerative medicine 140 and much more. Focusing on hydrogels in biomaterial applications, the water storing capability make hydrogels ideal for e.g.…”

Section: Chapter 6 Superstructures Polymeric Network and Hydrogelsmentioning

confidence: 99%

Tunable and modular assembly of polypeptides and polypeptide-hybrid biomaterials

Aronsson¹

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Biomaterials are materials that are speci cally designed to be in contact with biological systems and have for a long time been used in medicine. Examples of biomaterials range from sophisticated prostheses used for replacing outworn body parts to ordinary contact lenses. Currently it is possible to create biomaterials that can e.g. speci cally interact with cells or respond to certain stimuli. Peptides, the shorter version of proteins, are excellent molecules for fabrication of such biomaterials. By following and developing design rules it is possible to obtain peptides that can self-assemble into well-de ned nanostructures and biomaterials. e aim of this thesis is to create "smart" and tunable biomaterials by molecular self-assembly using dimerizing α-helical polypeptides. Two di erent, but structurally related, polypeptide-systems have been used in this thesis. e EKIV-polypeptide system was developed in this thesis and consists of four 28-residue polypeptides that can be mixed-and-matched to self-assemble into four di erent coiled coil heterodimers. e dissociation constant of the di erent heterodimers range from µM to < nM. Due to the large di erence in a nities, the polypeptides are prone to thermodynamic social self-sorting. e JR-polypeptide system, on the other hand, consists of several 42-residue de novo designed helix-loop-helix polypeptides that can dimerize into four-helix bundles. In this work, primarily the glutamic acid-rich polypeptide JR2E has been explored as a component in supramolecular materials. Dimerization was induced by exposing the polypeptide to either Zn 2+ , acidic conditions or the complementary polypeptide JR2K.By conjugating JR2E to hyaluronic acid and the EKIV-polypeptides to star-shaped poly(ethylene glycol), respectively, highly tunable hydrogels that can be self-assembled in a modular fashion have been created. In addition, self-assembly of spherical superstructures has been investigated and were obtained by linking two thiol-modi ed JR2E polypeptides via a disul de bridge in the loop region.e thesis also demonstrates that the polypeptides and the polypeptide-hybrids can be used for encapsulation and release of molecules and nanoparticles. In addition, some of the hydrogels have been explored for 3D cell culture. By using supramolecular interactions combined with bio-orthogonal I covalent crosslinking reactions, hydrogels were obtained that enabled facile encapsulation of cells that retained high viability.e results of the work presented in this thesis show that dimerizing α-helical polypeptides can be used to create modular biomaterials with properties that can be tuned by speci c molecular interactions. e modularity and the tunable properties of these smart biomaterials are conceptually very interesting and make them useful in many emerging biomedical applications, such as 3D cell culture, cell therapy, and drug delivery. II Populärvetenskaplig sammanfattningBiomaterialär material somär designade för a vara i kontakt med biologiskt material och har använts länge inom m...

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Hydrogels for tissue engineering and regenerative medicine

Cited by 325 publications

References 216 publications

3D bioprinting for tissue engineering: Stem cells in hydrogels

3D bioprinting for tissue engineering: Stem cells in hydrogels

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

Tunable and modular assembly of polypeptides and polypeptide-hybrid biomaterials

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