Hydrogen peroxide and lignification

Olson, Paul; Varner, J. E.

doi:10.1046/j.1365-313x.1993.04050887.x

Cited by 244 publications

(166 citation statements)

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“…This reagent was composed of 4% (w/v) starch and 100 mol m -3 KI (Olson & Varner 1993) adjusted to pH 5·0 with KOH. Areas of H 2 O 2 production were monitored by observing the development of a dark stain on the cut surface over a period of 10 h, which was sensitive to catalase (Ros Barceló 1998a).…”

Section: Histochemical Stain For Monitoring H 2 O 2 Productionmentioning

confidence: 99%

“…It is easily monitored by the starch/KI reagent (Olson & Varner 1993;Ros Barceló 1998a), which yields a brown stain sensitive to catalase. With this in mind, the effect of NO-releasing compounds on H 2 O 2 production by the lignifying xylem of Z. elegans was also studied in order to ascertain whether NO donors, which are inhibitors of peroxidase, are also capable of inhibiting H 2 O 2 production.…”

Section: Effect Of No-releasing Compounds On the Catalytic Activity Omentioning

confidence: 99%

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Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Ferrer

Barceló

1999

Plant Cell & Environment

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Sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) are two nitric oxide (NO)-releasing compounds that, when used at 5·0 mol m -3 concentrations, are capable of releasing NO in the aqueous phase at a rate of 35 ± 4 and 47 ± 5 µmol m -3 s -1 , respectively. For this reason, the effect of SNP and SNAP on coniferyl alcohol peroxidase and on H 2 O 2 production by the lignifying xylem of Zinnia elegans (L.) has been studied in order to ascertain whether NO, which is a synchronizing chemical messenger in animals and an air pollutant, has any effect on these plant-specific metabolic aspects. The results showed that both SNP and SNAP provoke an inhibition in the mol m -3 concentration range of the coniferyl alcohol peroxidase activity of a basic peroxidase isoenzyme present in the intercellular washing fluid of Z. elegans. The effect of these NO-releasing compounds on peroxidase was confirmed through histochemical studies, which showed that xylem peroxidase was totally inhibited by treatment with these NO donors at 5·0 mol m -3 , and by NO at a concentration change rate of 55 ± 5 and 110 ± 9 µmol m -3 s -1 . However, SNP, at 5·0 mol m -3 , does not have any effect on H 2 O 2 production by the xylem of Z. elegans. The fact that SNP and SNAP are two structurally dissimilar compounds which only share the common ability to release NO in aqueous buffer, and that similar results were obtained when using NO itself, suggest that NO could be considered as an inhibitor of coniferyl alcohol peroxidase which does not affect H 2 O 2 production in the xylem of Z. elegans.Key-words: Zinnia elegans; coniferyl alcohol oxidation; H 2 O 2 production; inhibition; nitric oxide; peroxidase; xylem.Abbreviations: IWF, intercellular washing fluid; pCMB, pchloromercuribenzoic acid; SNAP, S-nitroso-N-acetyl-penicillamine; SNP, sodium nitroprusside; TMB, 3,3',5,5'-tetramethylbenzidine. INTRODUCTIONNitric oxide (NO) is a paramagnetic relatively stable freeradical molecule involved in many physiological processes in animals, where it serves as a synchronizing chemical messenger involved in vasorelaxation, platelet inhibition, neurotransmission, cytotoxicity and immunoregulation (Anbar 1995). In animal cells, NO is synthesized by the enzyme NO synthase (Knowles 1997), and most of its biological regulatory properties have been explained on the basis of its capacity to act as an iron ligand in haemoproteins (Stamler, Singel & Loscalzo 1992). Dual (activating or inhibitory) effects of NO on haemoproteins have been described (Tsai 1994), and the nature of the effect has been suggested to depend to a great extent on the resting state (oxidation state) of the haemoprotein, which conditions the ligand properties and the electronic configuration of the haem iron (Tsai 1994). In the case of catalase, it has been shown that NO reversibly binds to the haem of catalase and inhibits H 2 O 2 breakdown with a K i of about 0·2 mmol m -3 (Brown et al. 1997).Evidence is emerging to support the possibility that NO may also act as chemical mess...

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Section: Histochemical Stain For Monitoring H 2 O 2 Productionmentioning

confidence: 99%

Section: Effect Of No-releasing Compounds On the Catalytic Activity Omentioning

confidence: 99%

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Ferrer

Barceló

1999

Plant Cell & Environment

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“…ROS are also generated in plant tissues in response to wounding (Angelini et al, 1990;Bradley et al, 1992;Olson and Varner, 1993;Felton et al, 1994;Bi and Felton, 1995;Orozco-Cárdenas and Ryan, 1999), mechanical stimulation of isolated cells (Yahraus et al, 1995;Gus-Mayer et al, 1998), and the treatment of cell suspension cultures with elicitors (Legendre et al, 1993;Mithö fer et al, 1997;Stennis et al, 1998). ROS also have been associated with plant herbivore interactions Leitner et al, 2005), and oxidative changes in the plants correspond with oxidative damage in the midguts of insects feeding on previously wounded plants (Orozco-Cárdenas and Ryan, 1999).…”

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Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

et al. 2006

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In response to herbivore (Spodoptera littoralis) attack, lima bean (Phaseolus lunatus) leaves produced hydrogen peroxide (H 2 O 2 ) in concentrations that were higher when compared to mechanically damaged (MD) leaves. Cellular and subcellular localization analyses revealed that H 2 O 2 was mainly localized in MD and herbivore-wounded (HW) zones and spread throughout the veins and tissues. Preferentially, H 2 O 2 was found in cell walls of spongy and mesophyll cells facing intercellular spaces, even though confocal laser scanning microscopy analyses also revealed the presence of H 2 O 2 in mitochondria/peroxisomes. Increased gene and enzyme activations of superoxide dismutase after HW were in agreement with confocal laser scanning microscopy data. After MD, additional application of H 2 O 2 prompted a transient transmembrane potential (V m ) depolarization, with a V m depolarization rate that was higher when compared to HW leaves. In transgenic soybean (Glycine max) suspension cells expressing the Ca 21 -sensing aequorin system, increasing amounts of added H 2 O 2 correlated with a higher cytosolic calcium ([Ca 21 ] cyt ) concentration. In MD and HW leaves, H 2 O 2 also triggered the increase of [Ca 21 ] cyt , but MD-elicited [Ca 21 ] cyt increase was more pronounced when compared to HW leaves after addition of exogenous H 2 O 2 . The results clearly indicate that V m depolarization caused by HW makes the membrane potential more positive and reduces the ability of lima bean leaves to react to signaling molecules.

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“…Within minutes of pathogen recognition, reactive oxygen species are generated that may promote cross-linking and lignification of the cell wall (2,3), transcription of defense-related genes (4,5), secondary metabolite biosynthesis (6), the hypersensitive response (4,7,8), and direct pathogen cytotoxicity (9,10), depending on the plant species examined. In cultured cell systems, the oxidative burst can be promoted by isolated elicitors including harpin (11,12), oligouronides (13), elicitins (14), purified fungal peptides (15), and other molecules from the extracts of pathogens (7,16).…”

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confidence: 99%

Measurement of Ca2+ Fluxes during Elicitation of the Oxidative Burst in Aequorin-transformed Tobacco Cells

Chandra

Low

1997

Journal of Biological Chemistry

148

117

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We have employed suspension cultured aequorintransformed tobacco cells to examine the involvement of Ca 2؉ in signal transduction of the oxidative burst. Use of cultured cells for this purpose was validated by demonstrating that the cells responded to cold shock quantitatively and qualitatively similarly to the intact transgenic plants from which they were derived. Stimulation of the oxidative burst in the cell suspension was achieved by administration of oligogalacturonic acid, Mas-7 (a peptide known to activate G proteins and Ca 2؉ fluxes), hypo-osmotic stress, or harpin (a protein from the pathogenic bacterium Erwinia amylovora). The latter failed to promote any detectable increase in cytoplasmic Ca 2؉ concentration, whereas each of the former three triggered a rapid rise in cytosolic Ca 2؉ followed by a return within seconds to basal Ca 2؉ levels. Peak Ca 2؉ concentrations induced by the former three elicitors were ϳ0.7, 1.4, and 1.3 M, respectively. Three lines of evidence suggest that the observed Ca 2؉ pulses are essential to transduction of the oxidative burst signals by their respective elicitors: (i) inhibition of the Ca 2؉ transients with Ca 2؉ chelators or Ca 2؉ channel blockers prevented expression of the oxidative burst, (ii) introduction of exogenous Ca 2؉ into the same cells initiated the burst even in the absence of other inducers of the response, and (iii) the observed Ca 2؉ transients often returned to near basal levels well before any H 2 O 2 synthesis could be detected, suggesting that the Ca 2؉ influx is required to communicate the burst signal but not maintain the defense response. These data suggest that Ca 2؉ pulses serve frequently, but not invariably, to transduce an oxidative burst signal.The oxidative burst constitutes one of the more rapid responses of a plant cell to pathogen attack (1). Within minutes of pathogen recognition, reactive oxygen species are generated that may promote cross-linking and lignification of the cell wall (2, 3), transcription of defense-related genes (4, 5), secondary metabolite biosynthesis (6), the hypersensitive response (4,7,8), and direct pathogen cytotoxicity (9, 10), depending on the plant species examined. In cultured cell systems, the oxidative burst can be promoted by isolated elicitors including harpin (11, 12), oligouronides (13), elicitins (14), purified fungal peptides (15), and other molecules from the extracts of pathogens (7,16). Abiotic stimuli such as mechanical stress (17), the pesticide fenthion (18), cold shock (19), phosphatase inhibitors (4, 20), and hypo-osmotic stress (17) can also induce biosynthesis of reactive oxygen species in plant cells.Because of its rapid expression, ease of assay, and similarity to the analogous defense response in human neutrophils (21), the oxidative burst has recently served as a model for exploring signal transduction pathways in plants. As the numbers of elicitors examined have risen, it has become increasingly clear that different elicitors may inaugurate independent signal transduction pathways that...

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Hydrogen peroxide and lignification

Cited by 244 publications

References 0 publications

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

Measurement of Ca2+ Fluxes during Elicitation of the Oxidative Burst in Aequorin-transformed Tobacco Cells

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Hydrogen peroxide and lignification

Cited by 244 publications

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Differential effects of nitric oxide on peroxidase and H2O2 production by the xylem of Zinnia elegans

Differential effects of nitric oxide on peroxidase and H2O2 production by the xylem of Zinnia elegans

Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

Measurement of Ca2+ Fluxes during Elicitation of the Oxidative Burst in Aequorin-transformed Tobacco Cells

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Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans