Hydrogen peroxide detection nanosensor array for biosensor development

Lupu, A.; Lisboa, Patrícia Cristina; Valsesia, Andrea; Colpo, Pascal; Rossi, François

doi:10.1016/j.snb.2008.10.004

Cited by 40 publications

(22 citation statements)

References 41 publications

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“…The increase in the cathodic current corresponds to the reduction of H 2 O 2 as a result of the electrocatalytic activity of the AgNPs on the SWCNT electrode. The reduction peak of H 2 O 2 was undefined; this is consistent with previous studies that reported a broad range of potentials for H 2 O 2 [56,57]. Figure 3 A further investigation was done to find out the transport characteristics of H 2 O 2 to the SWCNT/AgNPs modified electrode.…”

Section: Resultssupporting

confidence: 87%

Voltammetric and amperometric determination of hydrogen peroxide using a carbon-ceramic electrode modified with a nanohybrid composite made from single-walled carbon nanotubes and silver nanoparticles

2012

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A nanohybrid composite material was prepared from single-walled carbon nanotubes and silver nanoparticles, and used to fabricate a modified carbon-ceramic electrode. The preparation of the composite is facile and efficient. The nanohybrid composite deposited on the carbon-ceramic electrode was characterized by X-ray diffraction and cyclic voltammetry. The new electrode displays favorable electrocatalytic ability towards hydrogen peroxide (H 2 O 2 ) and can be used to electrocatalytically reduce this species. Under the optimum conditions, the current measured during hydrodynamic amperometry is linearly related to the concentration of H 2 O 2 over the concentration range from 0.01 to 8 mM, with a detection limit of 2×10 −7 M at a signal-to-noise ratio of 3 and sensitivity of 3.23 μA/mM. The electrode exhibits good reproducibility, long-term stability and negligible interference by dopamine, uric acid, and other important biological compounds. The electrode was successfully applied to the determination of H 2 O 2 in honey samples, and the recovery was 101.2%.

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Section: Resultssupporting

confidence: 87%

Voltammetric and amperometric determination of hydrogen peroxide using a carbon-ceramic electrode modified with a nanohybrid composite made from single-walled carbon nanotubes and silver nanoparticles

2012

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“…Biosensors based on horseradish peroxidase (HRP) have emerged as the most convenient tools for hydrogen peroxide determination [1][2][3][4]. It is well-known that immobilizing a biomolecule on the sensing electrode surface, and maintaining the enzyme activity is a crucial step in enzyme biosensor design [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Yuan

Zhuo

et al. 2010

Appl Biochem Biotechnol

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In this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (HRP) on SiO₂/BSA/Au/thionine/nafion-modified gold electrode was fabricated successfully. Firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (Thi) as an electron mediator via the ion-exchange interaction between the Thi and nafion. Subsequently, the SiO₂/BSA/Au composite nanoparticles were assembled onto Thi film through the covalent bounding with the amino groups of Thi. Finally, HRP was immobilized on the SiO₂/BSA/Au composite nanoparticles due to the covalent conjugation to construct an enzyme biosensor. The surface topographies of the SiO₂/BSA/Au composite nanoparticles were investigated by using scanning electronic microscopy. The stepwise self-assemble procedure of the biosensor was further characterized by means of cyclic voltammetry and chronoamperometry. The enzyme biosensor showed high sensitivity, good stability and selectivity, a wide linear response to hydrogen peroxide (H₂O₂) in the range of 8.0 x 10⁻⁶ ~3.72 x 10⁻³ mol/L, with a detection limit of 2.0 x 10⁻⁶ mol/L. The Michaelies-Menten constant K(app)(M) value was estimated to be 2.3 mM.

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“…A higher sensitivity was obtained by Lupu et al [93] using a new sensor platform based on nanoarrays of PPy nanopillars, obtained using nanosphere lithography. Polystyrene beads deposited on a gold surface were reduced in size by plasma etching, covered with an insulating layer, and subsequently removed, thus leaving nanoholes on the surface.…”

Section: Conductive Polymer Nanostructures Used In Catalytic Biosensorsmentioning

confidence: 92%

New Micro‐ and Nanotechnologies for Electrochemical Biosensor Development

Berti

Turner

2011

Biosensor Nanomaterials

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IntroductionOver the last decade, great attention has been paid to the inclusion of newly developed nanomaterials such as nanowires, nanotubes, and nanocrystals in sensor devices. This can be attributed to the ability to tailor the size and structure, and hence the properties of nanomaterials, thus opening up excellent prospects for designing novel sensing systems and enhancing the performance of bioanalytical assays [1] . Considering that most biological systems, including viruses, membranes, and protein complexes, are naturally nanostructured materials and that molecular interactions take place on a nanometer scale, nanomaterials are intuitive candidates for integration into biomedical and bioanalytical devices [2, 3] . Moreover, they can pave the way for the miniaturization of sensors and devices with nanometer dimensions (nanosensors and nanobiosensors) in order to obtain better sensitivity, specifi city, and faster rates of recognition compared to current solutions.The chemical, electronic, and optical properties of nanomaterials generally depend on both their dimensions and their morphology [4] . A wide variety of nanostructures have been reported in the literature for interesting analytical applications. Among these, organic and inorganic nanotubes, nanoparticles, and metal oxide nanowires have provided promising building blocks for the realization of nanoscale electrochemical biosensors due to their biocompatibility and technologically important combination of properties, such as high surface area, good electrical properties, and chemical stability. Moreover, the integration of nanomaterials in electrochemical devices offers the possibility of realizing portable, easy -to -use, and inexpensive sensors, due to the ease of miniaturization of both the material and the transduction system. Over the last decade, this fi eld has been extensively investigated and a huge number of papers have been published. This chapter principally summarizes progress made in the last few years (2005 to date) in the integration of nanomaterials such as carbon nanotubes (CNTs), nanoparticles, and polymer nanostructures in electrochemical biosensing systems.

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Hydrogen peroxide detection nanosensor array for biosensor development

Cited by 40 publications

References 41 publications

Voltammetric and amperometric determination of hydrogen peroxide using a carbon-ceramic electrode modified with a nanohybrid composite made from single-walled carbon nanotubes and silver nanoparticles

Voltammetric and amperometric determination of hydrogen peroxide using a carbon-ceramic electrode modified with a nanohybrid composite made from single-walled carbon nanotubes and silver nanoparticles

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

New Micro‐ and Nanotechnologies for Electrochemical Biosensor Development

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