Hydrogen peroxide enhanced indole-3-butyric acid effects on Cordia trichotoma adventitious rooting

Silva, Maria Kalyane Farias da; Siqueira, David Pessanha; Carvalho, Giovanna Campos Mamede Weiss de; Silva, Renata de Deus; Silva, Rachel Martins da Rocha; Barroso, Déborah Guerra

doi:10.1016/j.rhisph.2022.100533

Cited by 6 publications

(2 citation statements)

References 40 publications

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“…Arrab. Ex Steud mini-cuttings [ 66 ]. Furthermore, the application of 20–40 mM H 2 O 2 significantly increased the number of roots in cucumber seedling explants, while H 2 O 2 up to 50 mM resulted in increased root fresh weight [ 51 ].…”

Section: The Role and Function Of Hydrogen Peroxide In Root Formationmentioning

confidence: 99%

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

Roussos

2023

Antioxidants

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Adventitious root formation is defined as the formation of new roots on above-ground plant parts and is considered crucial for the survival of a plant under harsh environmental conditions (i.e., flooding, salt stress, and other abiotic stresses) as well as in the nursery industry. Clonal propagation is based on the ability of a plant part to grow and generate a completely new plant, genetically identical to the mother plant, where the plant part came from. Nurseries exploit this potential by multiplying millions of new plants. Most nurseries use cuttings to achieve that, through the induction of adventitious root formation. Many factors have been implicated in the capacity of a cutting to root, with the major role being played by auxins. During the last few decades, intense interest has emerged in the role of other potential rooting co-factors, such as carbohydrates, phenolics, polyamines, and other plant growth regulators, as well as signal molecules, such as reactive oxygen and nitrogen species. Among the latter, hydrogen peroxide and nitric oxide have been found to play significant roles in adventitious root formation. Their production, action, and general implication in rhizogenesis are discussed in this review, in terms of interaction with other molecules and signaling.

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Section: The Role and Function Of Hydrogen Peroxide In Root Formationmentioning

confidence: 99%

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

Roussos

2023

Antioxidants

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“…Cordia trichotoma es una especie arbórea de la familia Boraginaceae nativa del Bosque Atlántico, región que se distribuye en los países de Argentina, Brasil y Paraguay (da Silva et al, 2022). Es de gran valor ambiental y altamente apreciada en el comercio por su madera, debido a que posee buenas propiedades físicas y mecánicas (da Silva Zanchetta et al, 2020;Gonçalves et al, 2022).…”

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Establecimiento in vitro de semillas de una especie con importancia maderera y melífera del Bosque Atlántico

Duarte

2024

Revista Mutis

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Cordia trichotoma (Vell.) Arrab. ex Steud es una especie arbórea que crece naturalmente en los países de Argentina, Brasil y Paraguay, que se encuentra en estado de conservación vulnerable, debido a la sobreexplotación con fines comerciales por poseer una madera de interés industrial. Con el objetivo de establecer un protocolo de multiplicación in vitro de Cordia trichotoma, frutos con y sin escarificación física fueron sometidos a desinfección con peróxido de hidrógeno (H2O2) 30 volúmenes al 50% durante 5 días, etanol 70% durante un minuto e hipoclorito de sodio (NaClO) al 1 y 2% durante 20 minutos. Posteriormente, frutos o semillas fueron cultivados en medio basal compuesto de las sales minerales de Murashige y Skoog, con una concentración de sacarosa de 30 gr.L-1, semisólido (agar 6,5 gr.L-1), e incubados durante un mes a 27±2 °C y 16 horas de luz. El uso del H2O2 al 50% durante 5 días, combinado con diferentes concentraciones de NaClO y cultivando las semillas 0 frutos resulta en alto niveles de desinfección en donde más del 80% de los cultivos no presentaron contaminación visible, la CG fue del 33,33±23,09 al 90±10,00 % y el IVG fue de 0,13±0,09 a 0,57±0,03 semillas por día. El uso del H2O2 en la desinfección de frutos de C. trichotoma, es fundamental para eliminar la mayor cantidad de microorganismos que interfieren en el normal desarrollo de las plantas germinadas in vitro. La utilización de este desinfectante conjuntamente con el NaClO logró altos porcentajes de germinación, en tanto que la escarificación mecánica influye negativamente sobre el establecimiento de las semillas de Cordia trichotoma.

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Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

et al. 2023

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Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro- and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the mini cuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages.

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Hydrogen peroxide enhanced indole-3-butyric acid effects on Cordia trichotoma adventitious rooting

Cited by 6 publications

References 40 publications

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

Establecimiento in vitro de semillas de una especie con importancia maderera y melífera del Bosque Atlántico

Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

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