Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms

Raval, Yash S.; Mohamed, Abdelrhman; Flurin, Laure; Mandrekar, Jayawant N.; Quaintance, Kerryl E. Greenwood; Beyenal, Haluk; Patel, Robin

doi:10.1016/j.bioflm.2021.100055

Cited by 19 publications

(21 citation statements)

References 46 publications

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“…In previous work, we showed that this e‐bandage markedly reduced in vitro bacterial biofilms within 48 h of treatment. [ 30 ] Here, we assessed this H 2 O 2 producing e‐bandage in a mouse wound infection model.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Activity of Hydrogen‐Peroxide Generating Electrochemical Bandage Against Murine Wound Infections

Raval

Fleming

Mohamed

et al. 2023

Advanced Therapeutics

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Biofilms formed by antibiotic-resistant bacteria in wound beds challenge the treatment of wound infections. In this work, the activity of a novel electrochemical bandage (e-bandage) composed of carbon fabric and controlled by a wearable potentiostat, designed to continuously deliver low amounts of hydrogen peroxide (H 2 O 2 ), is evaluated against methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Pseudomonas aeruginosa (MDR-PA) and mixed-species (MRSA and MDR-PA) wound infections. Wounds created on Swiss Webster mice are infected with the above-named bacteria and biofilms allow to establish in wound beds for 3 days. e-Bandages, which electrochemically reduce dissolved oxygen to H 2 O 2 when polarized at −0.6 V Ag/AgCl , are placed atop infected wound beds and polarized continuously for 48 h. Polarized e-bandage treatment results in significant reductions (p <0.001) of both monospecies and mixed-species wound infections. After e-bandage treatment, electron microscopy shows degradation of bacterial cells, and histopathology shows no obvious alterations to the inflammatory host response. Blood biochemistries show no abnormalities. Taken all together, results of this work suggest that the described H 2 O 2 -producing e-bandage can reduce in vivo MRSA, MDR-PA, and mixed-species wound biofilms, and should be further developed as a potential antibiotic-free strategy for treatment of wound infections.

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Section: Discussionmentioning

confidence: 99%

In Vivo Activity of Hydrogen‐Peroxide Generating Electrochemical Bandage Against Murine Wound Infections

Raval

Fleming

Mohamed

et al. 2023

Advanced Therapeutics

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“…With the e‐bandage, an agar membrane biofilm model was used to simulate physical properties of the wound bed and validate the technology prior to animal testing. Polarized e‐bandages were active against mono‐ and dual‐species bacterial and yeast biofilms (Raval et al, 2022 ; Raval, Mohamed, et al, 2021 ). In this work, validation was extended to assessment of e‐bandages against ex vivo biofilms grown on porcine explants.…”

Section: Discussionmentioning

confidence: 99%

“…Substitution of a hydrogel for the liquid medium was used to transform e‐scaffolds into electrochemical bandages (e‐bandages) for direct application to wound surfaces. The e‐bandage reduced in vitro biofilms of mono‐ and dual‐species micro‐organisms, including methicillin‐resistant S. aureus , multidrug‐resistant A. baumannii and yeast (Mohamed et al, 2021 ; Raval et al, 2022 ; Raval, Mohamed, et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model

Tibbits

Mohamed

Gelston

et al. 2022

Journal of Applied Microbiology

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Aims: Effects of H 2 O 2 producing electrochemical-bandages (e-bandages) on methicillin-resistant Staphylococcus aureus colonization and biofilm removal were assessed using a porcine explant biofilm model. Transport of H 2 O 2 produced from the e-bandage into explant tissue and associated potential toxicity were evaluated. Methods and Results: Viable prokaryotic cells from infected explants were quantified after 48 h treatment with e-bandages in three ex vivo S. aureus infection models: (1) reducing colonization, (2) removing young biofilms and (3) removing mature biofilms. H 2 O 2 concentration-depth profiles in explants/biofilms were measured using microelectrodes. Reductions in eukaryotic cell viability of polarized and nonpolarized noninfected explants were compared. e-Bandages effectively reduced S. aureus colonization (p = 0.029) and reduced the viable prokaryotic cell concentrations of young biofilms (p = 0.029) with limited effects on mature biofilms (p > 0.1). H 2 O 2 penetrated biofilms and explants and reduced eukaryotic cell viability by 32-44% compared to nonpolarized explants. Conclusions: H 2 O 2 producing e-bandages were most active when used to reduce colonization and remove young biofilms rather than to remove mature biofilms. Significance and Impact of Study: The described e-bandages reduced S. aureus colonization and young S. aureus biofilms in a porcine explant wound model, supporting their further development as an antibiotic-free alternative for managing biofilm infections. K E Y W O R D S biofilm, electroceutical, electrochemical bandage, hydrogen peroxide, porcine explant, Staphylococcus aureus CONFLICT OF INTEREST H.B. holds a patent (US20180207301A1), 'Electrochemical reduction or prevention of infections', which refers to the electrochemical scaffold described herein. R.P. reports | 3765

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“…Biofilm Eradication Assay. 35,36 Biofilm eradication experiments were performed using a pegged-lid microtiter plate assay to determine the MBEC values for compounds of interest, as previously described. 1,2 Briefly, 125 mL of midlog phase culture diluted to ca.…”

Section: ■ Conclusionmentioning

confidence: 99%

Soft QPCs: Biscationic Quaternary Phosphonium Compounds as Soft Antimicrobial Agents

et al. 2023

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Quaternary ammonium compounds (QACs) serve as a first line of defense against infectious pathogens. As resistance to QACs emerges in the environment, the development of next-generation disinfectants is of utmost priority for human health. Balancing antibacterial potency with environmental considerations is required to effectively counter the development of bacterial resistance. To address this challenge, a series of 14 novel biscationic quaternary phosphonium compounds (bisQPCs) have been prepared as amphiphilic disinfectants through straightforward, high-yielding alkylation reactions. These compounds feature decomposable or “soft” amide moieties in their side chains, anticipated to promote decomposition under environmental conditions. Strong bioactivity against a panel of seven bacterial pathogens was observed, highlighted by single-digit micromolar activity for compounds P6P-12A,12A and P3P-12A,12A. Hydrolysis experiments in pure water and in buffers of varying pH revealed surprising decomposition of the soft QPCs under basic conditions at the phosphonium center, leading to inactive phosphine oxide products; QPC stability (>24 h) was maintained in neutral solutions. The results of this work unveil soft QPCs as a potent and environmentally conscious new class of bisQPC disinfectants.

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Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms

Cited by 19 publications

References 46 publications

In Vivo Activity of Hydrogen‐Peroxide Generating Electrochemical Bandage Against Murine Wound Infections

In Vivo Activity of Hydrogen‐Peroxide Generating Electrochemical Bandage Against Murine Wound Infections

Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model

Soft QPCs: Biscationic Quaternary Phosphonium Compounds as Soft Antimicrobial Agents

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