Hydrogen peroxide metabolism and functions in plants

Smirnoff, Nicholas; Arnaud, D

doi:10.1111/nph.15488

Cited by 701 publications

(496 citation statements)

References 202 publications

(227 reference statements)

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“…Moreover, the mitochondrial enzymes, 1-galactono-γlactone dehydrogenase and aconitase are the key enzymes for the indirect and direct formation of O2 •respectively (Kanojia and Dijkwel 2018). Beside chloroplasts and mitochondria, peroxisomes also produce ROS during stress and act as a major site for H2O2 generation (Smirnoff and Arnaud 2019). During stress, the mechanism behind switching high H2O2 generation is the low CO2 availability inside the plant leaves due to which ribulose-1,5-bisphosphate carboxylase/oxylase catalyzes a competitive reaction.…”

Section: Discussionmentioning

confidence: 99%

Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense response

Kumari¹,

Kaur²

2020

Biol. plant.

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Di-n-butyl phthalate (DBP) is one of the frequently detected phthalates in environmental samples. The effects of phthalates are extensively studied in the animals but the effects on plants are scarce. Therefore, the present study is aimed to envisage the effects of DBP on the antioxidative defense system in Hordeum vulgare L. seedlings grown under laboratory conditions for 7 d. The activities of different antioxidative enzymes were enhanced in the shoots. In the roots, the activity of guaiacol peroxidase increased and the catalase activity decreased initially but increased at higher DBP concentrations, whereas the activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase declined. Furthermore, the content of polyphenols elevated after exposure of seedlings to DBP. The possible reason for these responses of barley seedlings is the oxidative burst, i.e., enhanced production of reactive oxygen species, which were confirmed using confocal microscopy in terms of loss in plasma membrane integrity. DBP also disturbed the normal stomatal morphology of barley seedlings. The study may help to provide insights into the defense of crop plants against phthalate stress.

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Section: Discussionmentioning

confidence: 99%

Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense response

Kumari¹,

Kaur²

2020

Biol. plant.

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“…HL-induced damage of the photosynthetic electron transport chain is often related to the production of ROS such as singlet oxygen, which is mainly produced in PSII antenna, and superoxide anion and H 2 O 2 , which are mainly produced in the vicinity of PSI (Smirnoff and Arnaud, 2019). To simulate conditions of photoinhibition at the PSII side and induce overproduction of ROS, we treated leaf disks of Col-0 and cia2-2 cil-1 with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and methyl viologen (MV) and monitored F v /F m (Figure 3d).…”

Section: Characterization Of Cia2 Cil Responses In Chloroplast-targetmentioning

confidence: 99%

Dual-targeted transcription factors are required for optimal photosynthesis and stress responses in Arabidopsis thaliana

Gawroński

Burdiak

et al. 2019

Preprint

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SummaryChloroplast to nucleus retrograde signaling is essential for cell function, acclimation to fluctuating environmental conditions, plant growth and development. The vast majority of chloroplast proteins are nuclear-encoded and must be imported into the organelle after synthesis in the cytoplasm. This import is essential for the development of fully functional chloroplasts. On the other hand, functional chloroplasts act as sensors of environmental changes and can trigger acclimatory responses that influence nuclear gene expression. Signaling via mobile transcription factors (TFs) has been recently recognized as a way of communication between organelles and the nucleus. In this study, we performed a targeted reverse genetic screen to identify novel dual-localized TFs involved in chloroplast retrograde signaling during stress responses. We found that CHLOROPLAST IMPORT APPARATUS 2 (CIA2), a TF with putative plastid transit peptide can be detected in chloroplasts and the nucleus. Further, we found that CIA2, along with its homolog CIA2-like (CIL) act in an unequally redundant manner and are involved in the regulation of Arabidopsis responses to UV-AB, high light, and heat shock. Finally, our results suggest that both CIA2 and CIL are crucial for chloroplast translation. Our results contribute to a deeper understanding of signaling events in the chloroplast-nucleus cross-talk.SignificanceWe found that a transcription factor CIA2 can be located in chloroplasts and nucleus. CIA2 and is close homolog CIL are involved in protein translation and abiotic stress responses, and we suggest that they play an essential role in retrograde signaling between these organelles.

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“…Plants have evolved complex signal transduction networks that enable the sensing and integration of extracellular signals, and the processing and transduction of the underlying information into physiological-, growth- and developmental responses. Within such signaling networks, spatiotemporal concentration changes of hormones, the divalent cation Ca 2+ and reactive oxygen species (ROS) mediate various downstream responses (Dodd et al, 2010; Kudla et al, 2010; Shan et al, 2012; Vanstraelen and Benková, 2012; Mittler et al, 2017; Waszczak et al, 2018; Smirnoff and Arnaud, 2019). Among the plant hormones, abscisic acid (ABA) functions as a central regulator of the plant water status (Cutler et al, 2010; Finkelstein et al, 2013; Yoshida et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Dual-sensing genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second messenger dynamics in Arabidopsis

Waadt

Köster

Andrés

et al. 2019

Preprint

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Dual-sensing genetically encoded fluorescent indicators resolve the 22 spatiotemporal coordination of cytosolic abscisic acid and second messenger 23 dynamics in Arabidopsis 24 SHORT TITLE 25 Coordination of signaling compound fluxes in roots 26 Rainer Waadt et al. -Coordination of signaling compound fluxes in roots 2 27 ABSTRACT 28 Deciphering signal transduction processes is crucial for understanding 29 how plants sense and respond to environmental changes. Various chemical 30 compounds function as central messengers within deeply intertwined signaling 31 networks. How such compounds act in concert remains to be elucidated. We 32 have developed dual-sensing genetically encoded fluorescent indicators (2-In-1-33 GEFIs) for multiparametric in vivo analyses of the phytohormone abscisic acid 34 (ABA), Ca 2+ , protons (H + ), chloride (anions), the glutathione redox potential (E GSH ) 35 and hydrogen peroxide (H 2 O 2 ). Simultaneous analyses of two signaling 36 compounds in Arabidopsis (Arabidopsis thaliana) roots revealed that ABA 37 treatment and uptake did not trigger rapid cytosolic Ca 2+ or H + fluxes. Glutamate, 38 ATP, Arabidopsis PLANT ELICITOR PEPTIDE (AtPEP1) and glutathione disulfide 39 (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca 2+ , H + 40 and anion fluxes, but except for GSSG only weakly affected the cytosolic redox 41 state. Overall, 2-In-1-GEFIs enable complementary high-resolution in vivo 42 analyses of signaling compound dynamics and facilitate an advanced 43 understanding of the spatiotemporal coordination of signal transduction 44 processes in Arabidopsis. 45 46 198 Supplemental Movie 3). These experiments established the functionality of both 2-In-1-199 GEFIs and revealed that ABA does not trigger rapid cytosolic Ca 2+ or pH changes in 200 roots. 201 Auxin, ATP and glutamate treatments trigger spatiotemporally overlapping fluxes 202 of Ca 2+ , H + and Cl -203 Rainer Waadt et al. -Coordination of signaling compound fluxes in roots 8 Next, we used R-GECO1-GSL-E 2 GFP to simultaneously monitor Ca 2+ , H + and Cl -204 fluxes. Because anions, such as Cl -, quench the fluorescence of E 2 GFP and because its 205 excitation ratiometric pH readout is Clindependent, E 2 GFP provides a means to 206 simultaneously assess cytosolic H + and Clchanges (Bizzarri et al., 2006; Arosio et al., 207 2010). In response to 1 µM IAA, R-GECO1 reported biphasic Ca 2+ signals in the root 208 elongation zone that travelled to neighboring regions. Subsequent 1 mM ATP 209 treatments triggered Ca 2+ signals in the calyptra and meristematic zone that proceeded 210 shootward (Figure 4A left). Interestingly, both Ca 2+ signals coincided with a cytosolic 211 acidification reported by E 2 GFP (Figure 4A middle, Supplemental Movie 4). IAA also 212 induced a Clinflux, indicated by a E 2 GFP fluorescence emission decrease in the entire 213 imaged root, with a maximum decrease in the meristematic zone (Figure 4A right). ATP 214 however induced Clinflux in the upper elongation zone and above, but Clefflux ...

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Hydrogen peroxide metabolism and functions in plants

Cited by 701 publications

References 202 publications

Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense response

Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense response

Dual-targeted transcription factors are required for optimal photosynthesis and stress responses in Arabidopsis thaliana

Dual-sensing genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second messenger dynamics in Arabidopsis

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