Hydrogen peroxide yields in water radiolysis by high-energy ion beams at constant LET

Wasselin-Trupin, Virginie; Baldacchino, Gérard; Bouffard, S.; Hickel, B.

doi:10.1016/s0969-806x(01)00682-x

Cited by 61 publications

(39 citation statements)

References 29 publications

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“…H 2 O 2 yield increases with LET32, due to inter-track recombination of radicals and multiple ionisation events3334, but appears to be independent of dose rate35. However, for high LET particles, the yields of H 2 O 2 for the same LET but for different particles can be very different, suggesting that LET alone may not be sufficient to correctly characterise the energy deposition structure36. Indeed it has been demonstrated by fast spectroscopy, that the yield of short lived radicals (e aq − , · OH, · H, HO 2 · ) in response to water irradiation with pulsed heavy ions decreases with increasing LET1314.…”

Section: Discussionmentioning

confidence: 99%

Ultra-short laser-accelerated proton pulses have similar DNA-damaging effectiveness but produce less immediate nitroxidative stress than conventional proton beams

Raschke

Spickermann

Toncian

et al. 2016

Sci Rep

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Ultra-short proton pulses originating from laser-plasma accelerators can provide instantaneous dose rates at least 107-fold in excess of conventional, continuous proton beams. The impact of such extremely high proton dose rates on A549 human lung cancer cells was compared with conventionally accelerated protons and 90 keV X-rays. Between 0.2 and 2 Gy, the yield of DNA double strand breaks (foci of phosphorylated histone H2AX) was not significantly different between the two proton sources or proton irradiation and X-rays. Protein nitroxidation after 1 h judged by 3-nitrotyrosine generation was 2.5 and 5-fold higher in response to conventionally accelerated protons compared to laser-driven protons and X-rays, respectively. This difference was significant (p < 0.01) between 0.25 and 1 Gy. In conclusion, ultra-short proton pulses originating from laser-plasma accelerators have a similar DNA damaging potential as conventional proton beams, while inducing less immediate nitroxidative stress, which probably entails a distinct therapeutic potential.

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Section: Discussionmentioning

confidence: 99%

Ultra-short laser-accelerated proton pulses have similar DNA-damaging effectiveness but produce less immediate nitroxidative stress than conventional proton beams

Raschke

Spickermann

Toncian

et al. 2016

Sci Rep

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“…In the case of averaged LET measurements, the evolution of G‐value as function of LET is calculated from averaged values of G by differentiating an empirical function . This method is typically used for measurements of G‐values of hydrated electrons, hydroxyl radical, H‐atoms, and hydrogen peroxide …”

Section: Methodsmentioning

confidence: 99%

“…27 This method is typically used for measurements of G-values of hydrated electrons, hydroxyl radical, H-atoms, and hydrogen peroxide. [49][50][51][52][53][54] The yields of species from irradiation by electrons, protons, alpha particles, carbon ions, and silicon ions of varying energies were modeled using Geant4 to find the effects of the LET of charged particles on the initial and primary yields and the evolution of yields over time for each of the individual species. Further, the hydroxyl radical yield at 100 ns as a function of LET modeled by Geant4 was compared to experimental measurements.…”

Section: B Analysismentioning

confidence: 99%

Validation and investigation of reactive species yields of Geant4‐DNA chemistry models

et al. 2018

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Purpose Indirect biological damage due to reactive species produced in water radiolysis reactions is responsible for the majority of biological effect for low linear energy transfer (LET) radiation. Modeling water radiolysis and the subsequent interactions of reactive species, as well as track structures, is essential to model radiobiology on the microscale. Recently, chemistry models have been developed for Geant4‐DNA to be used in combination with the comprehensive existing physics models. In the current work, the first detailed, independent, in silico validation of all species yields with published experimental observations and comparison with other radiobiological simulations is presented. Additionally, the effect of LET of protons and heavier ions on reactive species yield in the model was examined, as well as the completeness of the chemical reactions following the radiolysis within the time after physical interactions simulated in the model. Methods Yields over time of reactive species were simulated for water radiolysis by incident electrons, protons, alpha particles, and ions with various LETs using Geant4 and RITRACKS simulation tools. Water dissociation and recombination was simulated using Geant4 to determine the completeness of chemical reactions at the end of the simulation. Yield validation was performed by comparing yields simulated using Geant4 with experimental observations and other simulations. Validation was performed for all species for low LET radiation and the solvated electron and hydroxyl radical for high LET ions. Results It was found that the Geant4‐DNA chemistry yields were generally in good agreement with experimental observations and other simulations. However, the Geant4‐DNA yields for the hydroxyl radical and hydrogen peroxide at the end of the chemistry stage were found to be respectively considerably higher and lower than the experimentally observed yields. Increasing the LET of incident hadrons increased the yield of secondary species and decreased the yield of primary species. The effect of LET on the yield of the hydroxyl radical at 100 ns simulated with Geant4 was in good agreement with experimental measurements. Additionally, by the end of the simulation only 40% of dissociated water molecules had been recombined and the rate of recombination was slowing. Conclusions The yields simulated using Geant4 are within reasonable agreement with experimental observations. Higher LET radiation corresponds with increased yields of secondary species and decreased yields of primary species. These trends combined with the LET having similar effects on the 100 ns hydroxyl radical yield for Geant4 and experimental measurements indicate that Geant4 accurately models the effect of LET on radiolysis yields. The limited recombination within the modeled chemistry stage and the slowing rate of recombination at the end of the stage indicate potential long‐range indirect biological damage.

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“…18) The direct consequence is a decrease of the amount of diffusible °OH radicals in the solution, resulting in a decrease of the SSB yield by free radicals attacks. Similar conclusions concerning the role of the free radicals °OH in SSB induction have been drawn by Jones et al in experiments made with helium ions 19) at different concentrations of DMSO.…”

Section: Dnamentioning

confidence: 99%

Comparison of DNA Breaks at Entrance Channel and Bragg Peak Induced by Fast C6+ Ions

Usami

Kobayashi

Hirayama³

et al. 2010

JRR

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When energetic carbon ion beam (GeV range) goes through the matter, inelastic processes such as electronic ionization, molecular and nuclear fragmentation occur. For carbontherapy (hadrontherapy) purpose, it is of interest to compare the number of DNA breaks -single SSB or double DSB- for a given dose at the entrance channel and at the Bragg peak to look for a possible differential effect in the number of DNA breaks induced at these two locations. Samples of free plasmids DNA and complexes of plasmids DNA added with molecules containing platinum have been placed at different locations of an experimental setup simulating penetration depths of the ion beam in water and irradiated by carbon ions 290 MeV/amu. The DNA breaks have been quantified by subsequent electrophoresis on agarose gels. To disentangle the respective role of the direct and indirect effect, a free radical scavenger of hydroxyl radicals HO degree-dimethylsulfoxide DMSO- has been added in some of the experiments. In the range of Linear Energy Transfer-LET 13 - 110 keV/microm-, the number of the DSB was found to be constant versus the LET for a given dose. Contrary, the number of the SSB decreases at the Bragg peak compared to the entrance channel. In the presence of platinum, the number of single and double breaks was considerably enhanced, and follows a similar behaviour than in the free-DNA experiments. Quantitative results on DNA damages do not show significant enhancement due to the nuclear or to the molecular fragmentation in the present experiments.

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Hydrogen peroxide yields in water radiolysis by high-energy ion beams at constant LET

Cited by 61 publications

References 29 publications

Ultra-short laser-accelerated proton pulses have similar DNA-damaging effectiveness but produce less immediate nitroxidative stress than conventional proton beams

Ultra-short laser-accelerated proton pulses have similar DNA-damaging effectiveness but produce less immediate nitroxidative stress than conventional proton beams

Validation and investigation of reactive species yields of Geant4‐DNA chemistry models

Comparison of DNA Breaks at Entrance Channel and Bragg Peak Induced by Fast C6+ Ions

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