Hydrogen photoproduction by three different nitrogenases in whole cells of Anabaena variabilis and the dependence on pH

Tsygankov, Anatoly A.; Serebryakova, Larissa T.; Sveshnikov, Dmitry; Rao, K. K.; Гоготов, И. Н.; Hall, D.O.

doi:10.1016/s0360-3199(96)00242-x

Cited by 46 publications

(23 citation statements)

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“…production. For example, it is known that immobilized cells produce more hydrogen than do free-living cultures (125) and that non-Mo-containing nitrogenases allocate more electrons to the production of hydrogen (206). The gas phase (214), the age and density of the culture, and the composition, pH, and temperature of the growth medium are also crucial to the final result.…”

Section: Cyanobacterial Biohydrogenmentioning

confidence: 99%

“…The gas phase (214), the age and density of the culture, and the composition, pH, and temperature of the growth medium are also crucial to the final result. Most of the research on cyanobacterial hydrogen production has been carried out with nitrogen-fixing strains (31,55,106,111,166,205,206). In these organisms, the net hydrogen production is the result of hydrogen evolution catalyzed by nitrogenase and of hydrogen consumption catalyzed mainly by an uptake hydrogenase.…”

Section: Cyanobacterial Biohydrogenmentioning

confidence: 99%

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Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

466

372

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Cyanobacteria may possess several enzymes that are directly involved in dihydrogen metabolism: nitrogenase(s) catalyzing the production of hydrogen concomitantly with the reduction of dinitrogen to ammonia, an uptake hydrogenase (encoded by hupSL) catalyzing the consumption of hydrogen produced by the nitrogenase, and a bidirectional hydrogenase (encoded by hoxFUYH) which has the capacity to both take up and produce hydrogen. This review summarizes our knowledge about cyanobacterial hydrogenases, focusing on recent progress since the first molecular information was published in 1995. It presents the molecular knowledge about cyanobacterial hupSL and hoxFUYH, their corresponding gene products, and their accessory genes before finishing with an applied aspect—the use of cyanobacteria in a biological, renewable production of the future energy carrier molecular hydrogen. In addition to scientific publications, information from three cyanobacterial genomes, the unicellular Synechocystis strain PCC 6803 and the filamentous heterocystous Anabaena strain PCC 7120 and Nostoc punctiforme (PCC 73102/ATCC 29133) is included

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Section: Cyanobacterial Biohydrogenmentioning

confidence: 99%

Section: Cyanobacterial Biohydrogenmentioning

confidence: 99%

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

466

372

View full text Add to dashboard Cite

show abstract

“…Mo was replaced by V because Vnitrogenase containing cells showed high activity and stability in hydrogen photoproduction [9]. The cultures were grown at 113 WE m 3P s 3I using daylight £uorescent lights, pH 7.0 (controlled by automated addition of 0.2 N NaOH), 30³C, with a gas mixture of 2% CO P +98% air (0.5 l min 3I ).…”

Section: Growth Conditionsmentioning

confidence: 99%

Acetylene reduction and hydrogen photoproduction by wild-type and mutant strains of Anabaena at different CO2 and O2 concentrations

Tsygankov¹

1998

FEMS Microbiology Letters

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Hydrogen photoproduction by growing cultures of Anabaena variabilis and A. azollae did not occur under air+CO P or argon+CO P atmospheres at saturating light but did take place under argon alone. It was shown that CO P inhibited photoproduction of H P as a result of the photosynthetic production of O P whereas photoreduction of C P H P by these cyanobacteria was not inhibited by O P concentrations up to 20% in the assay gas phase. In contrast to the wild type of A. variabilis and of A. azollae, H P photoproduction by the hydrogenase-impaired mutant A. variabilis PK84 showed only a slight dependence on O P concentration. Thus, in the wild-type Anabaena the decrease in the observed rate of H P evolution at elevated O P concentrations could be the result of an increase in hydrogenase-mediated uptake of H P via an oxyhydrogen reaction. z

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“…It has been shown that A. variabilis PK84 has essential advantages over the native strain in terms of H 2 production (Mikheeva et al, 1995;Sveshnikov et al, 1997;Tsygankov et al, 1998b) and that it can continuously evolve H 2 during cultivation in CO 2 -enriched air in photobioreactors under batch-growth conditions and continuous illumination (Markov et al, 1997;Tsygankov et al, 1999). In the present study we used vanadium-grown cells of the cyanobacterium, as it has been shown that the replacement of Mo by V in the medium enhances hydrogen production activity of A. variabilis (Kentemich et al, 1991;Tsygankov et al, 1997) and grew the cells in continuous culture under simulated day-night conditions in air.…”

Section: Introductionmentioning

confidence: 99%