Hydrogen‐producing <scp><i>E</i></scp><i>scherichia coli</i> strains overexpressing lactose permease: <scp>FT</scp>‐<scp>IR</scp> analysis of the lactose‐induced stress

Grūbe, Māra; Dimanta, Ilze; Gavare, Marita; Strazdiņa, Inese; Liepiņš, Jānis; Juhna, Tālis; Kalnenieks, Uldis

doi:10.1002/bab.1128

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…This experiment confirmed that little or no lactose molecule penetrates inside lon cells by either other facilitated transport systems or diffusion and LacY is required for the disaccharide internalization ( Figure 10 A). However, a deleterious effect on cell viability was observed in lacY + cells, resembling a “lactose killing” phenomenon [ 46 ]. Surprisingly, the comparison of the growth behaviors of recombinant KrPL lacY + bearing pP79- lacZ and pP79 highlights that the cause of the observed stress is not related to the de-energization caused by elevated transmembrane lactose transport.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Pseudoalteromonas haloplanktis TAC125 as a Cell Factory: IPTG-Inducible Plasmid Construction and Strain Engineering

et al. 2020

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Our group has used the marine bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125) as a platform for the successful recombinant production of “difficult” proteins, including eukaryotic proteins, at low temperatures. However, there is still room for improvement both in the refinement of PhTAC125 expression plasmids and in the bacterium’s intrinsic ability to accumulate and handle heterologous products. Here, we present an integrated approach of plasmid design and strain engineering finalized to increment the recombinant expression and optimize the inducer uptake in PhTAC125. To this aim, we developed the IPTG-inducible plasmid pP79 and an engineered PhTAC125 strain called KrPL LacY+. This mutant was designed to express the E. coli lactose permease and to produce only a truncated version of the endogenous Lon protease through an integration-deletion strategy. In the wild-type strain, pP79 assured a significantly better production of two reporters in comparison to the most recent expression vector employed in PhTAC125. Nevertheless, the use of KrPL LacY+ was crucial to achieving satisfying production levels using reasonable IPTG concentrations, even at 0 °C. Both the wild-type and the mutant recombinant strains are characterized by an average graded response upon IPTG induction and they will find different future applications depending on the desired levels of expression.

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Section: Discussionmentioning

confidence: 99%

Improvement of Pseudoalteromonas haloplanktis TAC125 as a Cell Factory: IPTG-Inducible Plasmid Construction and Strain Engineering

et al. 2020

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“…The FT-IR spectrum can be used for both qualitative and quantitative analyses of chemical components and is thus highly informative for analysis of multicomponent samples. FT-IR spectroscopy is thus a wellregarded analytical method for evaluation, monitoring and analysis of various biotechnological processes [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Application of FT-IR Spectroscopy for Investigation of Pink Water Remediation by Pine Bark

Grūbe¹,

Chusova²,

Gavare³

et al. 2015

TOBIOTJ

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Abstract. This study demonstrates the application of FT-IR spectroscopy for investigating the remediation of pink water with the low cost adsorbent pine bark. The removal of 2,4,6-trinitrotoluene (TNT) from pink water by adsorption to pine bark was accompanied by a reduction in intensities of peaks at 1544 and 1347 cm -1 in the spectra of acetonitrile extracts of the pine bark. Hierarchial cluster analysis differentiated samples with high (30-180 mg/L) and low (0-4 mg/L) TNT concentrations, demonstrating the potential of this approach as a quick screening method for the control of the removal of TNT from pink water. The amount of lignin in pine bark was inversely proportional to the size of the pine bark particles, while the concentration of phenolic hydroxyl groups increases with increasing size of pine bark particles. FT-IR spectra showed that as well as TNT, pine bark can also adsorb nitramine explosives such as RDX and HMX.

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Application of Anaerobic Digestate as Inoculum for Treatment of the Plant-Derived Wastes: Case Study

Kalneniece¹,

Bērziņš²,

Petrina³

et al. 2017

Materials Science and Applied Chemistry

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-The aim of this study was to characterize the digestate from an activated sludge biogas reactor (IDARS) as an inoculum for enzymatic hydrolysis and biogas production from organic wastes. Aerobic and anaerobic bacteria are represented in IDARS with H' index 3.1 and 2.5, respectively. IDARS stimulated the enzymatic hydrolysis of wood and leaves, preferably broadleaf tree species. The results demonstrated the potential of IDARS for improvement of the technological parameters for conversion of organic wastes into biogas.

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Hydrogen‐producing Escherichia coli strains overexpressing lactose permease: FT‐IR analysis of the lactose‐induced stress

Cited by 3 publications

References 23 publications

Improvement of Pseudoalteromonas haloplanktis TAC125 as a Cell Factory: IPTG-Inducible Plasmid Construction and Strain Engineering

Improvement of Pseudoalteromonas haloplanktis TAC125 as a Cell Factory: IPTG-Inducible Plasmid Construction and Strain Engineering

Application of FT-IR Spectroscopy for Investigation of Pink Water Remediation by Pine Bark

Application of Anaerobic Digestate as Inoculum for Treatment of the Plant-Derived Wastes: Case Study

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