Hydrogen-Rich Saline is Cerebroprotective in a Rat Model of Deep Hypothermic Circulatory Arrest

Abstract

Deep hypothermic circulatory arrest (DHCA) has been widely used in the operations involving the aortic arch and brain aneurysm since 1950s; but prolonged DHCA contributes significantly to neurological deficit which remains a major cause of postoperative morbidity and mortality. It has been reported that hydrogen exerts a therapeutic antioxidant activity by selectively reducing hydroxyl radical. In this study, DHCA treated rats developed a significant oxidative stress, inflammatory reaction and apoptosis. The administration of HRS resulted in a significant decrease in the brain injury, together with lower production of IL-1β, TNF-α, 8-OHdG and MDA as well as decreased activity of NOS while increased activity of SOD. The apoptotic index as well as the expressions of caspase-3 in brain tissue was significantly decreased after treatment. HRS administration significantly attenuated the severity of DHCA induced brain injury by mechanisms involving amelioration of oxidative stress, down-regulation of inflammatory factors and reduction of apoptosis.

Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury

“…( 12 ) Several researchers also observed that H 2 reduces oxidative DNA damage in brain tissues, indicating its potential as a neuroprotective potential. ( 26 – 28 ) In addition, H 2 can penetrate the blood-brain barrier, an import advantage of using H 2 over other neuroprotective drugs. ( 16 ) We have previously confirmed that maternal H 2 administration increases the H 2 concentrations in the fetal brains.…”

The transfer of hydrogen from inert gas to therapeutic gas

“…According to previous studies, the most common injection methods of hydrogen included inhaling hydrogen gas, intravenous or intraperitoneal injecting hydrogen solution, and drinking hydrogen rich water (HRW). 11 12 13 However, the above methods may not be able to guarantee enough hydrogen concentration in certain local damage models. Shigeta et al 14 demonstrated the luminal injection of hydrogen-rich glucose saline may be a novel and promising way in a small bowel ischemic/reperfusion (I/R) injury model.…”

Inhibition of microRNA-29c protects the brain in a rat model of prolonged hypothermic circulatory arrest

“…6 Oxidative stress and apoptotic neuronal death signaling pathways also have been involved in the brain in patients undergoing CPB and DHCA. 7 Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) is a potent stimulator of mitochondrial biogenesis and respiration, and powerfully regulates oxidative and mitochondrial metabolism in a number of tissues, including the central nerve system. 8 However, it is still not known whether PGC-1a is expressed in the brain after DHCA and functions as a regulator of oxidative stress and apoptotic neuronal death.…”

“…A pathologic score was used to evaluate the neurologic damage: 0 ¼ no damage; 1 ¼ 0% to 12.5% damage; 2 ¼ 12.5% to 25% damage; 3 ¼ 25% to 50% damage; and 4 ¼ more than 50% damage. 7 microRNA Extraction and Quantitative Reverse Transcription Polymerase Chain Reaction Total RNA was isolated by using Trizol reagent (Invitrogen, Carlsbad, Calif) according to the manufacturer's instruction. The first-strand cDNA was generated using the Reverse Transcription System Kit (Invitrogen).…”