Hydrogen-rich water promotes the formation of bulblets in Lilium davidii var. unicolor through regulating sucrose and starch metabolism

Hou, Xuemei; Qi, Ning; Wang, Chunlei; Li, Changxia; Huang, Dengjing; Li, Yihua; Wang, Ni; Liao, Weibiao

doi:10.1007/s00425-021-03762-6

Cited by 26 publications

(12 citation statements)

References 47 publications

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“…Starch and sucrose metabolisms hold central positions in carbohydrate metabolism 28 . Among the 135 genes in the starch and sucrose metabolism pathway, eight genes were up‐regulated after AVM exposure in the gastrointestinal tract.…”

Section: Resultsmentioning

confidence: 99%

“…Starch and sucrose metabolisms hold central positions in carbohydrate metabolism. 28 Among the 135 genes in the starch and sucrose metabolism pathway, eight genes were up-regulated after AVM exposure in the gastrointestinal tract. Among them, the alpha glucosidase, endo-beta-1,4-glucanase and cellulase GHF9 genes had very high fold changes (FC > 10) (Table 2, Supporting Information TableS5, Fig.…”

Section: Degs Associated With Stress-response and Metabolism Pathwaysmentioning

confidence: 99%

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Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

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BACKGROUND: Pesticides are extensively applied globally. Pesticide residues induce calamitous effects on the environment and untargeted organisms. Public concerns for the safety of freshwater organisms and the challenges posed by aquatic contaminants remain high. In the present study, the acute toxicity of avermectins (AVMs) to the crayfish, Procambarus clarkii was evaluated. We also evaluated the potential effects of AVM on the biochemical and transcriptomic status of the hepatopancreas and gastrointestinal tract in P. clarkii. RESULTS:The 24, 48, 72, 96 h median lethal concentrations (LC 50 ) of AVM on crayfish were 2.626, 1.162, 0.723, 0.566 mg L −1 , respectively. The crayfish were then exposed to 0.65 mg L −1 of AVM for 96 h. AVM significantly altered biochemical parameters including AChE and CAT activities in the hepatopancreas, and AChE, SOD and Na + -K + -ATPase activities in the gastrointestinal tract at several time points. Furthermore, transcriptomic analysis identified 953 and 1851 differentially-expressed genes (DEGs) in the hepatopancreas and gastrointestinal tract, respectively. KEGG enrichment showed that the gene expression profiles of the hepatopancreas and gastrointestinal tract were distinct from each other. The DEGs in the hepatopancreas were mostly enriched with stress-response pathways, while the majority of the DEGs in the gastrointestinal tract belonged to metabolism-related pathways.CONCLUSION: We demonstrated that the AVM induced acute toxicity, oxidative stress, osmoregulation disturbance, neurotoxicity and transcriptome imbalance in crayfish. These findings unraveled the detrimental effects of AVMs exposure on crayfish.

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Section: Resultsmentioning

confidence: 99%

Section: Degs Associated With Stress-response and Metabolism Pathwaysmentioning

confidence: 99%

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

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“…Compared with the CTAB method, the Kit method resulted in higher yield and purity RNA from bulb scales that contain high levels of polysaccharides and polyphenols. Previous studies reported that Kit method could be used to isolate RNA from bulb of Lanzhou lily 20 , 21 . However, the Kit protocol is not suitable for extracting total RNA from root, stem and leaf of Lilium in the current study (Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

Wang

Hou

et al. 2022

Sci Rep

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The high quality, yield and purity total RNA samples are essential for molecular experiments. However, harvesting high quality RNA in Lilium davidii var. unicolor is a great challenge due to its polysaccharides, polyphenols and other secondary metabolites. In this study, different RNA extraction methods, namely TRIzol method, the modified TRIzol method, Kit method and cetyltrimethylammonium bromide (CTAB) method were employed to obtain total RNA from different tissues in L. davidii var. unicolor. A Nano drop spectrophotometer and 1% agarose gel electrophoresis were used to detect the RNA quality and integrity. Compared with TRIzol, Kit and CTAB methods, the modified TRIzol method obtained higher RNA concentrations from different tissues and the A260/A280 ratios of RNA samples were ranged from 1.97 to 2.27. Thus, the modified TRIzol method was shown to be the most effective RNA extraction protocol in acquiring RNA with high concentrations. Furthermore, the RNA samples isolated by the modified TRIzol and Kit methods were intact, whereas different degrees of degradation happened within RNA samples isolated by the TRIzol and CTAB methods. In addition, the modified TRIzol method could also isolate high-quality RNA from other edible lily bulbs. Taken together, the modified TRIzol method is an efficient method for total RNA isolation from L. davidii var. unicolor.

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“…In this study, carbohydrate metabolism and amino acid metabolism were found to be significantly enriched ( p < 0.05). Starch metabolism and sucrose metabolism are the main carbohydrate metabolism of R. chrysanthum , which takes place through two different pathways [ 22 , 23 ]. The first pathway occurs in the chloroplast is starch synthesis.…”

Section: Discussionmentioning

confidence: 99%

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

Sun

Liu

Chen

et al. 2022

Plants

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Under natural environmental conditions, excess UV-B stress can cause serious injuries to plants. However, domestication conditions may allow the plant to better cope with the upcoming UV-B stress. The leaves of Rhododendron chrysanthum are an evergreen plant that grows at low temperatures and high altitudes in the Changbai Mountains, where the harsh ecological environment gives it different UV resistance properties. Metabolites in R. chrysanthum have a significant impact on UV-B resistance, but there are few studies on the dynamics of their material composition and gene expression levels. We used a combination of gas chromatography time-of-flight mass spectrometry and transcriptomics to analyze domesticated and undomesticated R. chrysanthum under UV-B radiation. A total of 404 metabolites were identified, of which amino acids were significantly higher and carbohydrates were significantly lower in domesticated R. chrysanthum. Transcript profiles throughout R. chrysanthum under UV-B were constructed and analyzed, with an emphasis on sugar and amino acid metabolism. The transcript levels of genes associated with sucrose and starch metabolism during UV-B resistance in R. chrysanthum showed a consistent trend with metabolite content, while amino acid metabolism was the opposite. We used metabolomics and transcriptomics approaches to obtain dynamic changes in metabolite and gene levels during UV-B resistance in R. chrysanthum. These results will provide some insights to elucidate the molecular mechanisms of UV tolerance in plants.

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Hydrogen-rich water promotes the formation of bulblets in Lilium davidii var. unicolor through regulating sucrose and starch metabolism

Cited by 26 publications

References 47 publications

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

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