Hydrogen sulfide alleviated chromium toxicity in wheat

Abstract

Effects of H 2 S on seed germination under chromium (Cr) stress were investigated in wheat (Triticum aestivum L.). Under Cr stress, the percentage of germination of wheat seeds decreased, but this decrease could be alleviated by pretreatment with NaHS, an H 2 S donor, in a dose-dependent manner. Furthermore, NaHS significantly enhanced the activities of amylase, esterase, superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase in Cr-stressed germinating seeds, whereas reduced the Cr-induced increase in lipoxygenase activity and over-production of malondialdehyde (MDA) and H 2 O 2 , and sustained slightly higher content of endogenous H 2 S.

Effect of pretreatment with hydrogen sulfide donor sodium hydrosulfide on heat tolerance in relation to antioxidant system in maize (Zea mays) seedlings

“…In present study, pretreatment with NaHS enhanced survival percentage of 2.5-dayold maize seedlings in a concentration-dependent manner (Fig. 1), consistent with the results of Zhang et al (2009bZhang et al ( , 2010a in wheat and sweetpotato under Cr and osmotic stress. All of above-mentioned studies indicated that H 2 S may be relative to the acquisition of stress tolerance including heat tolerance in plants, but their mechanisms are not fully clear.…”

“…In cucumber seedlings, total and isozymatic activities and corresponding transcripts of SOD, CAT, GPX and APX were activated differentially by NaHS, thus resulting in the alleviation of oxidative damage induced by boron (Wang et al 2010. Pretreatment with exogenous NaHS enhanced the activities of SOD, CAT, APX and GPX in wheat (Triticum aestivum L.) seedlings, reduced the Cr-induced increase in over-production of MDA and H 2 O 2 , followed by alleviated decrease in the germination rate of wheat seeds under Cr stress in a dose-dependent manner (Zhang et al 2010a). Again, application of NaHS increased the activities of APX, GR, dehydroascorbate reductase (DHAR) and γ-glutamylcysteine synthetase (γ-GCS), a key enzyme of GSH biosynthesis, as well as the contents of AsA, GSH, total ascorbate and total glutathione under water stress, which in turn decreased the MDA accumulation induced by water deficiency in wheat compared to control without NaHS treatment (Shan et al 2011).…”

“…In cucumber (Cucumis sativus) seedlings, root elongation was significantly inhibited by exposure of cucumber seedlings to solutions containing 5 mM boron (B), but this inhibitory effect was substantially alleviated by treatment with NaHS, a hydrogen sulfide donor (Wang et al 2010). In addition, under chromium (Cr) stress, the percentage of germination of wheat seeds decreased, but this decrease could be alleviated by pretreatment with NaHS in a dose-dependent manner (Zhang et al 2010a). Exposure of alfalfa (Medicago sativa) to 100 mM NaCl, seed germination and thereafter seedling growth were inhibited, but this negative effects could significantly be attenuated by NaHS at 100 µM .…”

“…In plant systems, hydrogen sulfide has been found to play multiple physiological roles in plant growth, development and the acquisition of stress tolerance like heat tolerance (Lisjak et al 2010(Lisjak et al , 2013Li 2013). The novel functions of hydrogen sulfide have been uncovered in seed germination (Zhang et al 2010a;Li et al 2012a), root organogenesis (Zhang et al 2009a) and stomata movement (Lisjak et al 2010;Liu et al 2011;Jin et al 2013), as well as in alleviation in osmotic stress (Zhang et al 2009b), salt stress (Wang et al 2012), oxidative stress (Shan et al 2011, heavy metal stress (Zhang et al 2008(Zhang et al , 2010bChen et al 2013) and pathogen infection (Bloem et al 2011). Our previous results also showed that pretreatment with sodium hydrosulfide (NaHS), a hydrogen sulfide donor, could improve resistance of tobacco cells (Li et al 2012b) and maize seedlings (Li et al 2013a(Li et al ,b, 2014 to high temperature, but its mechanism is not fully clear.…”

Hydrogen sulfide alleviates chlorobenzene toxicity in soybean seedlings

“…It is therefore the third discovered gaseous signaling molecule, following the discoveries of Nitric oxide (NO) and Carbon monoxide (CO). In plants, H 2 S is mainly produced via the L-/D-cysteine desulfhydrase enzymes (LCD/ DCD) and it has been reported to be involved in stress responses to drought, heat, heavy metal and salt during seed germination and seedling growth (ZHANG et al, 2010a;JIN et al, 2011;LAI et al, 2014;LI et al, 2014). Applications of H 2 S could also significantly improve root morphology, chlorophyll content and photosynthetic activity under Pb stress (ALI et al, 2014).…”