No abstract

For a long time, hydrogen sulfide (H2S) has been considered as merely a toxic by product of cell metabolism, but nowadays is emerging as a novel gaseous signal molecule, which participates in seed germination, plant growth and development, as well as the acquisition of stress tolerance including cross-adaptation in plants. Cross-adaptation, widely existing in nature, is the phenomenon in which plants expose to a moderate stress can induce the resistance to other stresses. The mechanism of cross-adaptation is involved in a complex signal network consisting of many second messengers such as Ca2+, abscisic acid, hydrogen peroxide and nitric oxide, as well as their crosstalk. The cross-adaptation signaling is commonly triggered by moderate environmental stress or exogenous application of signal molecules or their donors, which in turn induces cross-adaptation by enhancing antioxidant system activity, accumulating osmolytes, synthesizing heat shock proteins, as well as maintaining ion and nutrient balance. In this review, based on the current knowledge on H2S and cross-adaptation in plant biology, H2S homeostasis in plant cells under normal growth conditions; H2S signaling triggered by abiotic stress; and H2S-induced cross-adaptation to heavy metal, salt, drought, cold, heat, and flooding stress were summarized, and concluded that H2S might be a candidate signal molecule in plant cross-adaptation. In addition, future research direction also has been proposed.

It is well known that glutamate (Glu), a neurotransmitter in human body, is a protein amino acid. It plays a very important role in plant growth and development. Nowadays, Glu has been found to emerge as signaling role. Under normal conditions, Glu takes part in seed germination, root architecture, pollen germination, and pollen tube growth. Under stress conditions, Glu participates in wound response, pathogen resistance, response and adaptation to abiotic stress (such as salt, cold, heat, and drought), and local stimulation (abiotic or biotic stress)-triggered long distance signaling transduction. In this review, in the light of the current opinion on Glu signaling in plants, the following knowledge was updated and discussed. 1) Glu metabolism; 2) signaling role of Glu in plant growth, development, and response and adaptation to environmental stress; as well as 3) the underlying research directions in the future. The purpose of this review was to look forward to inspiring the rapid development of Glu signaling research in plant biology, particularly in the field of stress biology of plants.

Nitric oxide (NO) is a second messenger with multifunction that is involved in plant growth, development and the acquisition of stress tolerance. In recent years, hydrogen sulphide (H2S) has been found to have similar functions, but crosstalk between NO and H2S in the acquisition of heat tolerance is not clear. In this study, pretreatment with the NO donor sodium nitroprusside (SNP) improved the survival percentage of maize seedlings and alleviated an increase in electrolyte leakage and a decrease in tissue vitality as well as accumulation of malondialdehyde, indicating that pretreatment with SNP improved the heat tolerance of maize seedlings. In addition, pretreatment with SNP enhanced the activity of L-cystine desulfhydrase, which, in turn, induced accumulation of endogenous H2S, while application of H2S donors, NaHS and GYY4137, increased endogenous H2S content, followed by mitigating increase in electrolyte leakage and enhanced survival percentage of seedlings under heat stress. Interestingly, SNP-induced heat tolerance was enhanced by application of NaHS and GYY4137, but was eliminated by inhibitors of H2S synthesis DL-propargylglycine, aminooxyacetic acid, potassium pyruvate and hydroxylamine, and the H2S scavenger hypotaurine. All of the above-mentioned results suggest that SNP pretreatment could improve heat tolerance, and H2S may be a downstream signal molecule in NO-induced heat tolerance of maize seedlings.

Plants growing under field conditions are constantly exposed, either simultaneously or sequentially, to more than one abiotic stress factor. Plants have evolved sophisticated sensory systems to perceive a number of stress signals that allow them to activate the most adequate response to grow and survive in a given environment. Recently, cross-stress tolerance (i.e. tolerance to a second, strong stress after a different type of mild primary stress) has gained attention as a potential means of producing stress-resistant crops to aid with global food security. Heat or cold priming-induced cross-tolerance is very common in plants and often results from the synergistic co-activation of multiple stress signalling pathways, which involve reactive nitrogen species (RNS), reactive oxygen species (ROS), reactive carbonyl species (RCS), plant hormones and transcription factors. Recent studies have shown that the signalling functions of ROS, RNS and RCS, most particularly hydrogen peroxide, nitric oxide (NO) and methylglyoxal (MG), provide resistance to abiotic stresses and underpin cross-stress tolerance in plants by modulating the expression of genes as well as the post-translational modification of proteins. The current review highlights the key regulators and mechanisms underlying heat or cold priming-induced cross-stress tolerance in plants, with a focus on ROS, MG and NO signalling, as well as on the role of antioxidant and glyoxalase systems, osmolytes, heat-shock proteins (HSPs) and hormones. Our aim is also to provide a comprehensive idea on the topic for researchers using heat or cold priming-induced cross-tolerance as a mechanism to improve crop yields under multiple abiotic stresses.