Hydrogen sulfide donor sodium hydrosulfide-induced accumulation of betaine is involved in the acquisition of heat tolerance in maize seedlings

Li, Zhong-Guang; Zhu, L.-P.

doi:10.1007/s40415-014-0106-x

Cited by 34 publications

(13 citation statements)

References 46 publications

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“…However, the presence of H 2 S in the growth medium further enhanced Pro and GB content (Figure 3A,B) to a level that vigorously countered dehydration-induced osmotic stress, as reflected by enhanced LRWC and a lower rate of water loss in stressed plants (Figure 1A,B). An increment in Pro and GB content by H 2 S can be explained on the basis of the role of H 2 S in enhancing the activities of key enzymes responsible for Pro and GB synthesis [37,38]. Furthermore, H 2 S has been shown to increase the expression levels of abscisic acid (ABA) biosynthesis and reactivation genes [39], leading to stomatal closure [40].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill

Khan

Alzuaibr

Al-Huqail

et al. 2018

IJMS

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Hydrogen sulfide (H2S) has emerged as an important signaling molecule and plays a significant role during different environmental stresses in plants. The present work was carried out to explore the potential role of H2S in reversal of dehydration stress-inhibited O-acetylserine (thiol) lyase (OAS-TL), l-cysteine desulfhydrase (LCD), and d-cysteine desulfhydrase (DCD) response in arugula (Eruca sativa Mill.) plants. Dehydration-stressed plants exhibited reduced water status and increased levels of hydrogen peroxide (H2O2) and superoxide (O2•−) content that increased membrane permeability and lipid peroxidation, and caused a reduction in chlorophyll content. However, H2S donor sodium hydrosulfide (NaHS), at the rate of 2 mM, substantially reduced oxidative stress (lower H2O2 and O2•−) by upregulating activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and increasing accumulation of osmolytes viz. proline and glycine betaine (GB). All these, together, resulted in reduced membrane permeability, lipid peroxidation, water loss, and improved hydration level of plants. The beneficial role of H2S in the tolerance of plants to dehydration stress was traced with H2S-mediated activation of carbonic anhydrase activity and enzyme involved in the biosynthesis of cysteine (Cys), such as OAS-TL. H2S-treated plants showed maximum Cys content. The exogenous application of H2S also induced the activity of LCD and DCD enzymes that assisted the plants to synthesize more H2S from accumulated Cys. Therefore, an adequate concentration of H2S was maintained, that improved the efficiency of plants to mitigate dehydration stress-induced alterations. The central role of H2S in the reversal of dehydration stress-induced damage was evident with the use of the H2S scavenger, hypotaurine.

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Section: Discussionmentioning

confidence: 99%

Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill

Khan

Alzuaibr

Al-Huqail

et al. 2018

IJMS

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“…In maize seedlings, our previous studies also found that pretreatment with the NO donor sodium nitroprusside (SNP) (Li et al 2013a) or salicylic acid (SA) increased the survival percentage of maize seedlings by activating L-DES activity and endogenous H 2 S accumulation. Also, sodium hydrosulfide (NaHS), a H 2 S donor, can increase thermotolerance, and this effect is implicated in the accumulation of osmotic adjustment substances such as proline, trehalose and betaine as well as in enhancement of antioxidant system activity (Li et al 2013bLi and Zhu 2015). In addition, in tobacco suspension cell cultures, pretreatment with NaHS also can improve thermotolerance and the acquisition of this thermotolerance requires the mobilization of extracellular Ca 2?…”

Section: Introductionmentioning

confidence: 99%

Endogenous hydrogen sulfide regulated by calcium is involved in thermotolerance in tobacco Nicotiana tabacum L. suspension cell cultures

Long

Yang

et al. 2015

Acta Physiol Plant

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Calcium (Ca 2? )/calmodulin (CaM), a core component of calcium messenger system, is a multiple second messenger that is involved in multiple stress tolerance including heat tolerance in plants, hydrogen sulfide (H 2 S) is fast emerging similar functions, but Ca 2? and H 2 S crosstalk in the acquisition of thermotolerance is not completely understood. In this article, Ca 2? and CaM activated the activity of cysteine desulfhydrase (L-DES), followed by inducing endogenous H 2 S accumulation in tobacco suspension cultured cells, while treatment with Ca 2? chelator ethylene glycol-bis(b-aminoethylether)-N,N,N 0 ,N 0 -tetraacetic acid and CaM antagonists chlorpromazine lowered L-DES activity and H 2 S accumulation. Interestingly, Ca 2? -and CaM-activated L-DES activity and endogenous H 2 S accumulation were eliminated by H 2 S synthesis inhibitors DL-propargylglycine (PAG), aminooxy acetic acid (AOA), potassium pyruvate (PP) and hydroxylamine (HA), and the H 2 S scavenger hypotaurine (HT), indicating that Ca 2? and CaM regulated endogenous H 2 S generation via activating L-DES activity in tobacco cells. Furthermore, H 2 S donor NaHS, Ca 2? and CaM treatment alone significantly improved the thermotolerance of tobacco cells, and heat tolerance induced by Ca 2? and CaM was enhanced by exogenous NaHS, while weakened by PAG, AOA, PP, HA or HT. All above-mentioned results suggest that endogenous H 2 S generated by L-DES was involved, at least partly, in the thermotolerance induced by Ca 2? and CaM in tobacco suspension cell cultures.

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“…[11][12][13][14][15][16][17] Our previous research results also showed that NaHS treatment can improve heat tolerance of tobacco cells, maize and wheat seedlings. 1,[18][19][20][21][22][23][24] Enhancement in antioxidant defense system and osmotic adjustment are common mechanisms of plants adapt to adverse environments including heat stress by scavenging reactive oxygen species (ROS); buffering redox; stabilizing protein, nucleic acid and biomembrane; regulating acidity in cytoplasm. 25,26 Further study showed that SA and NaHS treatment alone or in combination significantly increased the activity of antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), catalase (CAT, SA treatment alone inhibited CT activity) and guaiacol peroxidase (GPX), and the content of antioxidants ascorbic acid (AsA) and glutathione (GSH), as well as the accumulation of endogenous osmolytes such as proline, betaine and trehalose in maize seedlings (Fig.…”

mentioning

confidence: 99%

Synergistic effect of antioxidant system and osmolyte in hydrogen sulfide and salicylic acid crosstalk-induced heat tolerance in maize (Zea mays L.) seedlings

2015

Plant Signaling & Behavior

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Salicylic acid (SA), is a plant hormone with multifunction that is involved in plant growth, development and the acquisition of stress tolerance. Hydrogen sulfide (H2S) is emerging similar functions, but crosstalk between SA and H2S in the acquisition of heat tolerance is not clear. Our recent study firstly reported that SA treatment enhanced the activity of L-cysteine desulfhydrase (L-DES), a key enzyme in H2S biosynthesis, followed by induced endogenous H2S accumulation, which in turn improved the heat tolerance of maize seedlings. (1) In addition, NaHS, a H2S donor, enhanced SA-induced heat tolerance, while its biosynthesis inhibitor DL-propargylglycine (PAG) and scavenger hydroxylamine (HT) weakened SA-induced heat tolerance. Also, NaHS had no significant effect on SA accumulation and its biosynthesis enzymes phenylalanine ammonia lyase (PAL) and benzoic-acid-2-hydroxylase (BA2H) activities, as well as significant difference was not observed in NaHS-induced heat tolerance of maize seedlings by SA biosynthesis inhibitors paclobutrazol (PAC) and 2-aminoindan-2-phosph- onic acid (AIP) treatment. (1) Further study displayed that SA induced osmolytes (proline, betaine and trehalose) accumulation and enhancement in activity of antioxidant system in maize seedlings. These results showed that antioxidant system and osmolyte play a synergistic role in SA and H2S crosstalk-induced heat tolerance of maize seedlings.

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Hydrogen sulfide donor sodium hydrosulfide-induced accumulation of betaine is involved in the acquisition of heat tolerance in maize seedlings

Cited by 34 publications

References 46 publications

Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill

Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill

Endogenous hydrogen sulfide regulated by calcium is involved in thermotolerance in tobacco Nicotiana tabacum L. suspension cell cultures

Synergistic effect of antioxidant system and osmolyte in hydrogen sulfide and salicylic acid crosstalk-induced heat tolerance in maize (Zea mays L.) seedlings

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