Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability

Liu, Yayu; Wei, Lijuan; Feng, Li; Zhang, Meiling; Hu, Dongliang; Tie, Jianzhong; Liao, Weibiao

doi:10.3390/plants11070935

Cited by 26 publications

(20 citation statements)

References 47 publications

(51 reference statements)

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“…Our previous study found that NO or H 2 O 2 treatment enhanced POD activity in chrysanthemum cuttings during AR initiation [ 19 ]. Sodium hydrosulfide (NaHS) treatment increased the activities of antioxidant enzymes (POD, superoxide dismutase, ascorbate peroxidase and catalase) and increased the contents of ascorbic acid and glutathione during rooting [ 54 ]. In this study, MRW, EBL and MRW + EBL treatments significantly enhanced POD activity during both the initiation phase (6-12 h) and expression phase (12-48 h), while Brz treatment significantly inhibited the positive effect (Fig.…”

Section: Discussionmentioning

confidence: 99%

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Hua²,

Hou

et al. 2023

BMC Plant Biol

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Background Methane (CH4) and brassinosteroids (BRs) are important signaling molecules involved in a variety of biological processes in plants. Results Here, marigold (Tagetes erecta L. ‘Marvel’) was used to investigate the role and relationship between CH4 and BRs during adventitious root (AR) formation. The results showed a dose-dependent effect of CH4 and BRs on rooting, with the greatest biological effects of methane-rich water (MRW, CH4 donor) and 2,4-epibrassinolide (EBL) at 20% and 1 μmol L− 1, respectively. The positive effect of MRW on AR formation was blocked by brassinoazole (Brz, a synthetic inhibitor of EBL), indicating that BRs might be involved in MRW-regulated AR formation. MRW promoted EBL accumulation during rooting by up-regulating the content of campestanol (CN), cathasterone (CT), and castasterone (CS) and the activity of Steroid 5α-reductase (DET2), 22α-hydroxylase (DWF4), and BR-6-oxidase (BR6ox), indicating that CH4 could induce endogenous brassinolide (BR) production during rooting. Further results showed that MRW and EBL significantly down-regulated the content of cellulose, hemicellulose and lignin during rooting and significantly up-regulated the hydrolase activity, i.e. cmcase, xylanase and laccase. In addition, MRW and EBL also significantly promoted the activity of two major cell wall relaxing factors, xyloglucan endotransglucosylase/hydrolase (XTH) and peroxidase, which in turn promoted AR formation. While, Brz inhibited the role of MRW on these substances. Conclusions BR might be involved in CH4-promoted AR formation by increasing cell wall relaxation.

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Section: Discussionmentioning

confidence: 99%

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Hua²,

Hou

et al. 2023

BMC Plant Biol

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“…Our results implied that suitable concentration of NO might alleviate the negative effect of Cd stress on the adventitious rooting of cucumber. Moreover, several studies indicated that NO could regulate the growth and development of plant roots, including root elongation, lateral root growth and adventitious root formation ( Díaz et al., 2021 ; Wang et al., 2021 ; Liu Y.Y. et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…The mixture is heated in a boiling water bath for 30 min and then centrifuged for 10 min. The absorbance of the supernatant was measured at 450 nm, 532 nm and 600 nm ( Liu et al, 2022b ). For estimating

generation, the samples were homogenized with potassium phosphate buffer (pH 7.8) and centrifuged for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

Niu

Tang²,

Zhu³

et al. 2023

Front. Plant Sci.

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Cadmium (Cd) as a potentially toxic heavy metal that not only pollutes the environment but also interferes with plant growth. Nitric oxide (NO) regulates plant growth and development as well as abiotic stress response. However, the mechanism underpinning NO-induced adventitious root development under Cd stress remains unclear. In this study, cucumber (Cucumis sativus ‘Xinchun No. 4’) was used as the experimental material to investigate the effect of NO on the development of adventitious roots in cucumber under Cd stress. Our results revealed that, as compared to Cd stress, 10 μM SNP (a NO donor) could considerably increase the number and length of adventitious roots by 127.9% and 289.3%, respectively. Simultaneously, exogenous SNP significantly increased the level of endogenous NO in cucumber explants under Cd stress. Our results revealed that supplementation of Cd with SNP significantly increased endogenous NO content by 65.6% compared with Cd treatment at 48 h. Furthermore, our study indicated that SNP treatment could improve the antioxidant capacity of cucumber explants under Cd stress by up-regulating the gene expression level of antioxidant enzymes, as well as reducing the levels of malondialdehyde (MDA), hydrogen peroxide (H2O2) and superoxide anion (O2.-) to alleviate oxidative damage and membrane lipid peroxidation. Application of NO resulted in a decrease of the O2.-, MDA, and H2O2 level by 39.6%, 31.4% and 60.8% as compared to Cd-alone treatment, respectively. Besides that, SNP treatment significantly increased the expression level of related genes involved in glycolysis processes and polyamine homeostasis. However, application of NO scavenger 2-(4-carboxy -2-phenyl)-4, 4, 5, 5-tetramethy limidazoline -1-oxyl -3-oxide (cPTIO) and the inhibitor tungstate significantly reversed the positive role of NO in promoting the adventitious root formation under Cd stress. These results suggest that exogenous NO can increase the level of endogenous NO, improve antioxidation ability, promote glycolysis pathway and polyamine homeostasis to enhance the occurrence of adventitious roots in cucumber under Cd stress. In summary, NO can effectively alleviate the damage of Cd stress and significantly promote the development of adventitious root of cucumber under Cd stress.

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“…However, the related genes in the POD activity were partially downregulated due to the prolonged NaCl stress. APX, GPX, and GR are important enzymes for scavenging intracellular ROS [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Antioxidant Mechanism of Tamarix ramosissima Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic

Chen

Zhang

et al. 2022

Antioxidants

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There is a serious problem with soil salinization that affects the growth and development of plants. Tamarix ramosissima Ledeb (T. ramosissima), as a halophyte, is widely used for afforestation in salinized soils. At present, there are few reports on the antioxidant mechanism of T. ramosissima under NaCl stress. In this study, we learned about the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, and hydrogen peroxide (H2O2) and malondialdehyde (MDA) content changes in T. ramosissima. We also mined the relevant metabolic pathways in the antioxidant mechanism, candidate key genes, and their related differential metabolites and verified them using quantitative real-time PCR (qRT-PCR). The results show that the SOD, POD, and CAT activities, and the H2O2 and MDA content reached the highest values in the roots of T. ramosissima. Simultaneously, 92 differentially expressed genes (DEGs) related to antioxidant enzyme activities changed during 48 and 168 h of NaCl stress, and these DEGs were mainly upregulated in 168 h. Based on the association analysis of transcriptomic and metabolomic data, we found Unigene0089358 and Unigene0007782 as genes related to key enzymes in the flavonoid biosynthesis pathway. They were located in the upstream positive regulation at 48 and 168 h under NaCl stress, and their respective related metabolites (phloretin and pinocembrin) were involved in resistance to NaCl stress, and they were significantly correlated with their respective metabolites. In conclusion, at 48 and 168 h under NaCl stress, the roots of T. ramosissima resist NaCl stress by enhancing enzymatic and nonenzymatic antioxidant mechanisms, scavenging ROS generated by high-salt stress, alleviating NaCl toxicity, and maintaining the growth of T. ramosissima. This study provides genetic resources and a scientific theoretical basis for further breeding of salt-tolerant Tamarix plants and the molecular mechanism of antioxidants to alleviate NaCl toxicity.

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Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability

Cited by 26 publications

References 47 publications

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

Analysis of the Antioxidant Mechanism of Tamarix ramosissima Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic

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