Hydrogen sulfide sensing using an aurone-based fluorescent probe

Abstract

Hydrogen sulfide detection and sensing is an area of interest from both an environmental and a biological perspective.

Aurone-derived 1,2,3-triazoles as potential fluorescence molecules in vitro

“…Since ionic detergents such as CTAB and sodium dodecyl-sulfate (SDS) have been shown to modulate the strength of fluorescent probes 20,45,46 and since detergent micelles have been used as membrane mimics for the study of membrane proteins, 47,48 it was decided that the interactions of AT5 with both ionic and neutral detergents should be characterized. To do this, various concentrations flanking the critical micelle concentration of CTAB, Triton X-100 (Triton), SDS, or Tween 20 (Tween) were separately mixed with 100 μM AT5 in PBS.…”

“…19 Most recently, Kafle et al in 2020 developed an aurone-derived sensor for hydrogen sulfide. 20 Though the potential for aurones as bioavailable fluorescent molecules has been reported, aurones traditionally only fluoresce in non-polar solvents and are quenched in aqueous environments. 12,13,20 Kafle et al were able to bypass the quenching effect of aqueous environments through the use of hexadecyltrimethylammonium bromide (CTAB).…”

“…12,13,20 Kafle et al were able to bypass the quenching effect of aqueous environments through the use of hexadecyltrimethylammonium bromide (CTAB). 20…”

An NBD tertiary amine is a fluorescent quencher and/or a weak green-light fluorophore in H2S-specific probes

“…In the last decade, many research groups have successfully developed hundreds of H 2 S fluorescent probes via different H 2 S-mediated reactions and sensing mechanisms. 7–18 For example, the H 2 S-induced reduction of azides has emerged as the most widely-used strategy for H 2 S probes since 2011. 8 Other reactions, including reduction of a nitro group and hydroxyamine, 9 thiolysis of dinitrophenyl ether, 10 various cleavages of C–O bonds, 11 C–S bonds, 12 and C–Cl bonds, 13 as well as Michael addition, 14 have also been used for H 2 S fluorescent probes.…”

A hydrostable CuII coordination network prepared hydrothermally as a “turn-on” fluorescent sensor for S2− and a selective adsorbent for methylene blue

“…5 It is well documented that long-term exposure to high concentrations of S 2− will result in serious environmental problems, as well as various physiological and biochemical diseases, such as respiratory paralysis, 6 Alzheimer's disease, 7 and Down's syndrome. 8 Many detection methods have been developed for the detection of S 2− in natural and physiological environments, such as gas chromatography (GC), 9 electrochemical analysis, 10 colorimetry, etc. 11 Nevertheless, these methods are usually not only limited by complex operation, monitoring delays and high detection limits, but also by the requirement for precision equipment and professionals.…”