Hydrogen sulfide stimulates β-amylase activity during early stages of wheat grain germination

Zhang, Hua; Dou, Wei; Jiang, Chengxi; Wei, Zhao‐Jun; Liu, Jian; Jones, Russell L.

doi:10.4161/psb.5.8.12297

Cited by 55 publications

(28 citation statements)

References 26 publications

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“…In plants, although originally associated with plant-pathogen interaction, it is now known to have an active role in diverse physiological processes such as oxidative stress, germination, and heat tolerance (Zhang et al, 2009a(Zhang et al, , 2009b(Zhang et al, , 2010a(Zhang et al, , 2010b(Zhang et al, , 2010dLi et al, 2013aLi et al, , 2013b. Recently, H 2 S has been proven to participate in ABA-or ethylene-induced stomatal closure in different plant species (García-Mata and Lamattina, 2010; Liu et al, 2011Liu et al, , 2012Jin et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Sulfide Generated byl-Cysteine Desulfhydrase Acts Upstream of Nitric Oxide to Modulate Abscisic Acid-Dependent Stomatal Closure

et al. 2014

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Abscisic acid (ABA) is a well-studied regulator of stomatal movement. Hydrogen sulfide (H 2 S), a small signaling gas molecule involved in key physiological processes in mammals, has been recently reported as a new component of the ABA signaling network in stomatal guard cells. In Arabidopsis (Arabidopsis thaliana), H 2 S is enzymatically produced in the cytosol through the activity of L-cysteine desulfhydrase (DES1). In this work, we used DES1 knockout Arabidopsis mutant plants (des1) to study the participation of DES1 in the cross talk between H 2 S and nitric oxide (NO) in the ABA-dependent signaling network in guard cells. The results show that ABA did not close the stomata in isolated epidermal strips of des1 mutants, an effect that was restored by the application of exogenous H 2 S. Quantitative reverse transcription polymerase chain reaction analysis demonstrated that ABA induces DES1 expression in guard cell-enriched RNA extracts from wild-type Arabidopsis plants. Furthermore, stomata from isolated epidermal strips of Arabidopsis ABA receptor mutant pyrabactin-resistant1 (pyr1)/pyrabactin-like1 (pyl1)/pyl2/pyl4 close in response to exogenous H 2 S, suggesting that this gasotransmitter is acting downstream, although acting independently of the ABA receptor cannot be ruled out with this data. However, the Arabidopsis clade-A PROTEIN PHOSPHATASE2C mutant abscisic acid-insensitive1 (abi1-1) does not close the stomata when epidermal strips were treated with H 2 S, suggesting that H 2 S required a functional ABI1. Further studies to unravel the cross talk between H 2 S and NO indicate that (1) H 2 S promotes NO production, (2) DES1 is required for ABA-dependent NO production, and (3) NO is downstream of H 2 S in ABA-induced stomatal closure. Altogether, data indicate that DES1 is a unique component of ABA signaling in guard cells.Abscisic acid (ABA) regulates diverse physiological and developmental processes in plants, among which seed dormancy and stomatal movement are the most studied. Stomata are pores bordered by pairs of specialized cells named guard cells located in the epidermis of the aerial part of most land plants. Due to the waxy cuticle of plants, stomatal pores regulate approximately 90% of all the gas exchange (i.e. the uptake of CO 2 required for photosynthesis and the loss of water vapor during transpiration) between the plant and the environment (Hetherington and Woodward, 2003). Thus, stomatal movement is a key process for the regulation of plant water status and biomass production. In guard cells, ABA induces an increase of intracellular calcium concentrations, which, in turn, induces an efflux of anions that causes membrane depolarization. In this voltage milieu, ion uptake is blocked through the inactivation of inward-rectifying potassium channels, and ion efflux is induced through activation of outward-rectifying potassium channels. This solute relocation drives water out of the guard cells and closes the stomatal pore as a result of a reduction in guard cell turgor (Blatt, 2000;Kim et al...

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Section: Discussionmentioning

confidence: 99%

Hydrogen Sulfide Generated byl-Cysteine Desulfhydrase Acts Upstream of Nitric Oxide to Modulate Abscisic Acid-Dependent Stomatal Closure

et al. 2014

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“…The quick activation of amylase and esterase during early stage of germination by H 2 S might contribute to the improved wheat germination under Cd stress. The possible mechanism of swift mobilization of amylase has been proposed in previous report that H 2 S might break the S-S bonds between b-amylase and activate it [27].…”

Section: Role Of Nahs On the Metabolism Of Germinating Wheat Grains Umentioning

confidence: 96%

Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress

Huang

Shao-can

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Hydrogen sulfide (H 2 S) has proved to be a multifunctional signaling molecule involved in plant development and response to abiotic stresses. In the present study, the effects of H 2 S donor, sodium hydrosulfide (NaHS) on the germination of wheat (Triticum aestivum L.) grains under cadmium stress was investigated. With the increase in Cd concentration, the germination percentage of wheat grains decreased steadily. NaHS pretreatment alleviated cadmium-induced germination inhibition in a dose-dependent manner, with the optimal concentration of 0.9 mM. Further studies showed that NaHS pretreatment could promote the activities of amylase and esterase, reducing cadmium-induced disturbance of plasma membrane integrity in the radicle tips, and sustain lower levels of superoxide radicals, hydrogen peroxide and malondialdehyde in germinating wheat grains. Besides, NaHS significantly promoted the accumulation of free amino acids in grains exposed to cadmium. Further investigation showed that NaHS pretreatment stimulated the activities of peroxidase, catalase and acorbate peroxidase and inhibited that of lipoxygenase. In addition, a higher level of endogenous H 2 S in wheat grains was observed in NaHSpretreated wheat grains. These data indicate that H 2 S ameliorated cadmium-stress in germinating wheat grains by modulating the antioxidant system.

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“…In Arabidopsis seedlings, the results of Shen et al (2013) showed that treating wild type with polyethylene glycol (PEG) 8000, to simulate drought stress, caused an increase in production rate of endogenous H 2 S (0.8 nmol mg -1 protein min -1 ). At early stage of osmotic exposure (PEG 6000 for 2 days), the endogenous H 2 S in wheat seeds rapidly increased from 1.5 to 3.5 μmol g -1 dry weight (DW) (Zhang et al, 2010a). …”

Section: H2s Signaling Triggered By Abiotic Stressmentioning

confidence: 99%

“…In strawberry seedlings, treatment of root with 0.1 mM NaHS resulted in significantly elevated H 2 S concentration (35 nmol g -1 FW) in leaves compared with control plants (25 nmol g -1 FW) (Christou et al, 2013). In wheat seeds, the endogenous H 2 S level [4.5 μmol g -1 dry weight (DW)] in NaHS-treated seed was slightly higher than that of control (1.7 mol g -1 DW) (Zhang et al, 2010a). These results indicated that H 2 S is easy to enter into plant cells and follow on being transported to other tissues or organs due to its highly lipophilic property, which in turn exert its physiological role in plants.…”

Section: H2s Signaling Triggered By Abiotic Stressmentioning

confidence: 99%

Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

2016

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For a long time, hydrogen sulfide (H2S) has been considered as merely a toxic by product of cell metabolism, but nowadays is emerging as a novel gaseous signal molecule, which participates in seed germination, plant growth and development, as well as the acquisition of stress tolerance including cross-adaptation in plants. Cross-adaptation, widely existing in nature, is the phenomenon in which plants expose to a moderate stress can induce the resistance to other stresses. The mechanism of cross-adaptation is involved in a complex signal network consisting of many second messengers such as Ca2+, abscisic acid, hydrogen peroxide and nitric oxide, as well as their crosstalk. The cross-adaptation signaling is commonly triggered by moderate environmental stress or exogenous application of signal molecules or their donors, which in turn induces cross-adaptation by enhancing antioxidant system activity, accumulating osmolytes, synthesizing heat shock proteins, as well as maintaining ion and nutrient balance. In this review, based on the current knowledge on H2S and cross-adaptation in plant biology, H2S homeostasis in plant cells under normal growth conditions; H2S signaling triggered by abiotic stress; and H2S-induced cross-adaptation to heavy metal, salt, drought, cold, heat, and flooding stress were summarized, and concluded that H2S might be a candidate signal molecule in plant cross-adaptation. In addition, future research direction also has been proposed.

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Hydrogen sulfide stimulates β-amylase activity during early stages of wheat grain germination

Cited by 55 publications

References 26 publications

Hydrogen Sulfide Generated byl-Cysteine Desulfhydrase Acts Upstream of Nitric Oxide to Modulate Abscisic Acid-Dependent Stomatal Closure

Hydrogen Sulfide Generated byl-Cysteine Desulfhydrase Acts Upstream of Nitric Oxide to Modulate Abscisic Acid-Dependent Stomatal Closure

Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress

Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

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