Hydrogen sulfide suppresses the expression of MMP-8, MMP-13, and TIMP-1 in left ventricles of rats with cardiac volume overload

Abstract

Aim: To study the effects of hydrogen sulfide (H 2 S) on the left ventricular expression of MMP-8, MMP-13, and TIMP-1 in a rat model of congenital heart disease. Methods: Male SD rats underwent abdominal aorta-inferior vena cava shunt operation. H 2 S donor NaHS (56 μmol·kg -1 ·d -1, ip) was injected from the next day for 8 weeks. At 8 weeks, the hemodynamic parameters, including the left ventricular systolic pressure (LVSP), the left ventricular peak rate of contraction and relaxation (LV±dp/dt max ) and the left ventricular end diastolic pressure (LVEDP) were measured. The left ventricular tissues were dissected out, and hydroxyproline and collagen I contents were detected with ELISA. The expression of MMP-8, MMP-13, and a tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues was measured using real-time PCR, Western blotting, and immunohistochemistry, respectively. Results: The shunt operation markedly reduced LVSP and LV±dp/dt max , increased LVEDP, hydroxyproline and collagen I contents, as well as the mRNA and protein levels of MMP-8, MMP-13, and TIMP-1 in the left ventricles. Chronic treatment of the shunt operation rats with NaHS effectively prevented the abnormalities in the hemodynamic parameters, hydroxyproline and collagen I contents, and the mRNA and protein levels of MMP-13 and TIMP-1 in the left ventricles. NaHS also prevented the increase of MMP-8 protein expression, but did not affect the increase of mRNA level of MMP-8 in the shunt operation rats. Conclusion: H 2 S suppresses protein and mRNA expression of MMP-8, MMP-13, and TIMP-1 in rats with cardiac volume overload, which may be contributed to the amelioration of ventricular structural remodeling and cardiac function.

Crude protein from spirulina increases the viability of CCD‑986sk cells via the EGFR/MAPK signaling pathway

“…In the present study, cell collagen production induced by SPCP treatment was 30-142% higher compared with the control group. The expression levels of the collagen-degrading protein MMP-8, which serves as a key enzyme in the degradation of collagen and stimulates the degradation of other major dermal components (which subsequently leads to aging) (41), was investigated by western blot analysis. These were decreased significantly in a dose-dependent manner in cells treated with SPCP.…”

Reverse Regulatory Pathway (H2S / PGE2 / MMP) in Human Aortic Aneurysm and Saphenous Vein Varicosity

“…Reversely, in mice cardiac tissues obtained following myocardial infarction, the observed decrease in CSE expression was associated with an increase in MMPs [ 30 ]. Other studies have also demonstrated that exogenous H 2 S sources (NaHS) led to reduction of MMP-13 and/or MMP-8 levels in human chondrocytes under inflammatory conditions [ 14 ] and in a model of rat heart disease [ 31 ].…”