The need for bioethanol each year increases steadily while raw materials tend to decline, this is due to bioethanol raw materials, so sought after materials that can be regenerated, bamboo is one of the alternative raw materials, Bamboo has a cellulose content ranging from 42.4%-53.6%. The chemical process in bioethanol cultivation includes the process of pretreatment, delignification, hydrolysis, and fermentation, it equipped with a micro-type controller Arduino with a fuzzy method using Delphi. Research results in the form of speed profiles, residual glucose levels, bioethanol function fermentation time. Fermentation time with fermiol optimum at 6 days, the highest yield of bioethanol 31.7%, the highest residual glucose concentration 3.9%, filtrate bamboo 250 ml. Result of running the Arduino type Controller microprogram with the fuzzy method using the Delphi language at the time of 137 seconds with a set point of 97 0C, as a result of running a comparison program from two controllers IE Auto-tuning PID with overshot 217 (0.08) slurries Time 5.26 and PID ACO with overshot 202 (0.018) slurries time 0.55 seconds. Of these two controllers can be selected PID ACO as the best controller with the fastest slurries time and the overshot is quite small.