Hydrolysis of oils in the Wadi Hanifah River in Saudi Arabia by free and immobilized Staphylococcus aureus ALA1 lipase

Abstract

This study describes the biochemical properties of Staphylococcus aureus lipase immobilized by physical adsorption to assess its role in the biodegradation of oils in the Wadi Hanifah River. After optimization of the immobilization conditions, the recovered enzyme activity was 95% with appreciable increase in stability. The immobilized and free lipase retained 70% and only 10% of the initial activity after a 60‐min incubation at 80 °C, respectively. More than ~40% residual activity remained after 48 h of incubation at pH5 to 11. The immobilized enzyme retained 100% of the initial activity when used for four cycles and 42% of initial activity when stored at 25 °C for 120 days. Enzyme stability was enhanced in the presence of inactivating agents including β‐mercaptoethanol, SDS, EDTA, and Co2+. The bioremediation potential of lipid‐rich wastewater by the immobilized and free lipases was explored by analyses of chemical oxygen demand and lipid content. Both free and immobilized lipases efficiently hydrolyzed all oils tested and organic matter present in the wastewater. Overall, the results indicate the potential of immobilized S. aureus lipase in biological wastewater treatment and offer new options for several industrial applications. © 2018 American Institute of Chemical Engineers Environ Prog, 38:e13000, 2019

Combined immobilized lipases for effective biodiesel production from spent coffee grounds

“…Candida rugosa lipase (CRL), CRL-B immobilized on an acrylic resin (Novozyme-435), porcine pancreatic lipase-II (PPL-II), and Rhizomucor miehei lipase (RML) were products of Sigma Aldrich (St. Louis, USA). Staphylococcus aureus (SAL) and Bacillus stearothermophilus (BSL) lipases were produced, partially purified and immobilized on CaCO 3 as described in previous works ( Ben Bacha et al, 2022b , Bacha et al, 2019 , Ben Bacha et al, 2016 , Bacha et al, 2015 ).…”

“…To overcome the drawbacks of chemical catalysts, lipases could be utilized as biocatalysts to produce biodiesel ( Karmee, 2016 , Karmee, 2018 ;). Interestingly, lipase enzymes are widely employed under moderate conditions as well as in conventional and nonconventional solvents, free fatty acid and moisture tolerant, and easy to recover ( Weldeslase et al, 2023 , Zou et al, 2023 , Ben Bacha et al, 2022a , Ben Bacha et al, 2022b , Binhayeeding et al, 2020 , Bacha et al, 2019 ). Microbial lipases, in particular, attracted a lot of attention with the recent advances in enzyme technology because they displayed numerous advantages such as the absence of seasonal changes, the continuous supply, high yield production in economically advantageous media besides the extensive variety of their catalytic activities.…”