Hydrolysis of whey by whole cells of Kluyveromyces bulgaricus immobilized in calcium alginate gels and in hen egg white

Biocatalysis with Immobilized Cells

“…In these capsules hybridoma cells could be otained in high density. Decleire et al (1985) mixed Kluyveromyces bulgaricus with hen egg white and treated the composite with glutaraldehyde; the hard gel so obtained was shattered by passage through a syringe and excess glutaraldehyde was washed off with distilled water. These immobilized cells were then used for the hydrolysis of whey lactose.…”

“…Mitani, Nishizawa and Nagai (1984a,b) observed that a mixture of photo-crosslinkable resin, K-carrageenan, locust bean gum and Celite together had the best mechanical and diffusion characteristics compared with those of any other combination of these components in the matrix. Decleire et al (1985) immobilized Saccharomyces cerevisiae cells by mixing with hen egg white and then treating the mixture with glutaraldehyde, as described previously (page 334). Cheetham, Garret and Clark (1985) immobilized Erwinia rhapontici cells on bone char by suspending it and the cells in water and adding tannic acid and glutaraldehyde to the mixture.…”

“…However, many people are unable to secrete lactase (13-galactosidase, EC 3.2.1.23) in their alimentary tract and, also, glucose and galactose are sweeter than lactose, so the hydrolysis of lactose in milk or whey is often desirable (Moulin and Galzy, 1984). Decleire et al (1985) achieved 80% conversion of lactose in whey with cells of a lactose producer, Kluyveromyces bulgaricus, immobilized in calcium alginate or egg white in a column reactor. At 4 C, the rate of hydrolysis was decreased by 10% after 13 days and by 20% after 17 days.…”