In order to facilitate water resources decisions, it is important that accurate and informative hydrometric data are collected. Combining information theory with multi-objective optimization has led to methods of optimizing the information content provided by hydrometric networks; however, there is no available study on the effects of spatial scale and data limitation on these methods. Herein, a dual entropy multi-objective optimization (DEMO) and a transinformation (TI) analysis were done to recommend optimal locations for additional hydrometric stations in the Madawaska Watershed. This analysis was designed to be comparative to a similar study conducted on the Ottawa River Basin which encompasses the Madawaska Watershed to allow for an investigation of the spatial scale effects in this type of network design. This study concludes that TI analysis is not adversely affected by scaling; however, the DEMO analysis is sensitive to the placement of potential station locations and the size of the study area. This study also examines the benefit of including nearby stations when the area of interest does not have a sufficient number of existing hydrometric stations for analysis. It is shown that these stations can provide useful information because their inclusion in the analysis increased the average TI in the watershed. Recommendations were made as to the ideal locations of additional stations in the Madawaska Watershed hydrometric network.(KEY TERMS: entropy; hydrometric network; multi-objective optimization; network design; water resources; spatial scale.) Werstuck, Connor and Paulin Coulibaly, 2018. Assessing Spatial Scale Effects on Hydrometric Network Design Using Entropy and Multi-objective Methods.