Hydrophilic, Bright CuInS<sub>2</sub> Quantum Dots as Cd-Free Fluorescent Labels in Quantitative Immunoassay

Speranskaya, E. S.; Beloglazova, Natalia V.; Abé, Sofie; Aubert, Tangi; Smet, Philippe; Poelman, Dirk; Goryacheva, Irina Yu.; Saeger, Sarah De; Hens, Zeger

doi:10.1021/la501268b

Cited by 85 publications

(60 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Currently, enzymes as labels have been widely used in commercially quantitative immunoassays [1, 2], especially enzyme-linked immunosorbent assay (ELISA) [3, 4] is frequently used in in vitro diagnosis because of its high sensitivity and specificity [5, 6]. The signal collection in ELISA are based on a change in optical transmittance by a time-dependent enzymatic reaction, but enzymes are usually vulnerable to external conditions and very susceptible to matrix constituent [7].…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Feng³

et al. 2017

J Nanobiotechnol

View full text Add to dashboard Cite

BackgroundThe conventional and widely used enzyme-linked immunosorbent assays (ELISA), due to specificity and high-sensitivity, were suitable in vitro diagnosis. But enzymes are vulnerable to the external conditions, and the complex operation steps limit its application. Semiconductor quantum dots have been successfully used in biological and medical research due to the high photoluminescence and high resistance to photobleaching. In this study, we have developed a novel quantum dot-labeled immunosorbent assay for rapid disease detection of C-reactive protein (CRP).ResultsThe assay for the detection of CRP can provide a wide analytical range of 1.56–400 ng/mL with the limit of detection (LOD) = 0.46 ng/mL and the limit of quantification = 1.53 ng/mL. The precision of the assay has been confirmed for low coefficient of variation, less than 10% (intra-assay) and less than 15% (inter-assay). The accuracy of assay meets the requirements with the recoveries of 95.4–105.7%. Furthermore, clinical samples have been collected and used for correlation analysis between this FLISA and gold standard Roche immunoturbidimetry. It shows excellent accurate concordance and the correlation coefficient value (R) is as high as 0.989 (n = 34).ConclusionsThis in vitro quantum dot-based detection method offers a lower LOD and a wide liner detection range than ELISA. The total reaction time is only 50 min, which is much shorter than the commercialization ELISA (about 120 min). All of the results show that a convenient, sensitive, and accurate fluorescence-linked immunosorbent assay method has been well established for the detection of CRP samples. Therefore, this method has immense potential for the development of rapid and cost-effective in vitro diagnostic kits.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0267-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Feng³

et al. 2017

J Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…CIS has the advantage of being a ternary material which allows further tuning of optical properties by changing the ratio of Cu and In in the lattice (De Trizio et al 2012;Nam et al 2011;Speranskaya et al 2014;Uehara et al 2008). For example, Cu poor CIS QDs have been shown to have increased PL quantum yield (PLQY) which was blue shifted compared to stoichiometric CIS (Nam et al 2011;Uehara et al 2008;Zhong et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…A good quality ZnS shell can be grown on the exposed CIS surfaces due to the small lattice mismatch between the two materials. ZnS shells have been shown to increase PLQY by passivating surface trap states on a range of QD systems including CdSe, CdZnSe, and CIS (Dabbousi et al 1997;Fitzmorris et al 2013;Li et al 2011;Park and Kim 2011;Speranskaya et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Structural and optical characterization of CuInS2 quantum dots synthesized by microwave-assisted continuous flow methods

et al. 2015

View full text Add to dashboard Cite

Semiconductor quantum dots (QDs) have recently been incorporated into consumer displays and lighting technologies. Now that these materials are being produced on industrial scales, it is important to investigate scalable synthetic methods and less toxic materials and chemistries. To achieve these goals, we have synthesized cadmium-free, visible light-emitting QDs using a microwave-assisted continuous flow reactor. After synthesis, the CuInS 2 QD cores underwent a near-complete Zn cation exchange reaction in a batch reactor, followed by the growth of a ZnS shell. Analysis of X-ray diffraction, transmission electron microscopy, and Raman spectroscopy data indicate that the crystal structure changes from CuInS 2 (chalcopyrite) to ZnS (zincblende) during the cation exchange reaction. Compositional analysis indicated that the core/shell QDs were *98 % ZnS, with Cu and In present at much lower concentrations. The photoluminescence (PL) peak position was blue shifted for longer cation exchange reactions, and it was found that the ZnS shell was necessary for improved PL stability.The synthesized QDs have a PL down conversion efficiency of *65 % when using a blue LED source.

show abstract

“…QDs, in particular, have emerged as one of the most promising labels for immunoassay detection because of their photostability and size tunable photoluminescence. They can be used to develop enzyme linked-immunosorbent assays (ELISAs) [4], lateral flow immunoassays (LFIAs) [99], Western blots [59] or in FRET studies [47,100], to name but a few. For biosensing, the greatest potential of QD-conjugates lies in multiplexing [51,99].…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays I-III-VI 2 semiconductor QDs (e.g. CuInS 2 ) are considered as one of the main alternatives of Cd-based QDs [4]. Due to QDs small size, the electrons are confined in a limited space, and when the radii of the semiconductor nanocrystal is smaller or equal than the excitation Bohr radius, there is quantization of the energy levels.…”

Section: Introductionmentioning

confidence: 99%

Bioconjugation of quantum dots: Review & impact on future application

Foubert

Beloglazova

Rajković

et al. 2016

TrAC Trends in Analytical Chemistry

Self Cite

120

110

View full text Add to dashboard Cite

Nowadays luminescent semiconductor quantum dots (QDs) are widely applied in different areas due to their unique optical properties. QDs can be used as photoluminescent labels with excellent possibilities for high-throughput detection and diagnostics. For most of such applications QDs must be coupled to biomolecules, which often represents a fundamental challenge. Although QDs have a lot of advantages over organic dyes, most of the techniques that have been developed for QD functionalization and bioconjugation, are more complicated than the corresponding techniques for organic fluorescent dyes. Here, the importance of choosing a suitable bioconjugation strategy in different applications, such as imaging and assays is described. The main goal of this review is to give a structured and detailed overview and comparison of the most widely used conjugation strategies in function of the active groups (carboxyl, amine, thiol, epoxy, hydroxyl and aldehyde groups) present on QD surface.

show abstract

Hydrophilic, Bright CuInS₂ Quantum Dots as Cd-Free Fluorescent Labels in Quantitative Immunoassay

Cited by 85 publications

References 51 publications

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Structural and optical characterization of CuInS2 quantum dots synthesized by microwave-assisted continuous flow methods

Bioconjugation of quantum dots: Review & impact on future application

Contact Info

Product

Resources

About

Hydrophilic, Bright CuInS2 Quantum Dots as Cd-Free Fluorescent Labels in Quantitative Immunoassay

Cited by 85 publications

References 51 publications

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Structural and optical characterization of CuInS2 quantum dots synthesized by microwave-assisted continuous flow methods

Bioconjugation of quantum dots: Review & impact on future application

Contact Info

Product

Resources

About

Hydrophilic, Bright CuInS₂ Quantum Dots as Cd-Free Fluorescent Labels in Quantitative Immunoassay