Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from <i>Pectinatus</i> Bacterium

Řezanka, Tomáš; Siříšťová, Lucie; Matoulková, Dagmar; Sigler, Karel

doi:10.1007/s11745-011-3556-y

Cited by 30 publications

(25 citation statements)

References 48 publications

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“…), whose base peak in the spectrum of PE plasmalogens shows ions of type [M+Li‐43] + due to their aziridine loss (Řezanka et al . ) from the adduct of molecular ion, e.g. [M+Li] + .…”

Section: Resultsmentioning

confidence: 99%

Lipidomics as an important key for the identification of beer-spoilage bacteria

Řezanka

Matoulková

Benada

et al. 2015

Lett Appl Microbiol

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Electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was used for characterizing intact plasmalogen phospholipid molecules in beer-spoilage bacteria. Identification of intact plasmalogens was carried out using collision-induced dissociation and the presence of suitable marker molecular species, both qualitative and quantitative, was determined in samples containing the anaerobic bacteria Megasphaera and Pectinatus. Using selected ion monitoring (SIM), this method had a limit of detection at 1 pg for the standard, i.e. 1-(1Z-octadecenyl)-2-oleoyl-sn-glycero-3-phosphoethanolamine and be linear in the range of four orders of magnitude from 2 pg to 20 ng. This technique was applied to intact plasmalogen extracts from the samples of contaminated and uncontaminated beer without derivatization and resulted in the identification of contamination of beer by Megasphaera and Pectinatus bacteria. The limit of detection was about 830 cells of anaerobic bacteria, i.e. bacteria containing natural cyclopropane plasmalogenes (c-p-19:0/15:0), which is the majority plasmalogen located in both Megasphaera and Pectinatus. The SIM ESI-MS method has been shown to be useful for the analysis of low concentration of plasmalogens in all biological samples, which were contaminated with anaerobic bacteria, e.g. juice, not only in beer. Significance and impact of the study: Electrospray ionization-tandem mass spectrometry (ESI-MS/MS) using collision-induced dissociation was used to characterize intact plasmalogen phospholipid molecules in beer-spoilage anaerobic bacteria Megasphaera and Pectinatus. Using selected ion monitoring (SIM), this method has a detection limit of 1 pg for the standard 1-(1Z-octadecenyl)-2-oleoyl-sn-glycero-3-phosphoethanolamine and is linear within four orders of magnitude (2 pg to 20 ng). The limit of detection was about 830 cells of bacteria containing natural cyclopropane plasmalogen (c-p-19:0/15:0). SIM ESI-MS method is useful for analyzing low concentrations of plasmalogens in biological samples contaminated with anaerobic bacteria, e.g. beer or juice.

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“…), whose base peak in the spectrum of PE plasmalogens shows ions of type [M+Li‐43] + due to their aziridine loss (Řezanka et al . ) from the adduct of molecular ion, e.g. [M+Li] + .…”

Section: Resultsmentioning

confidence: 99%

Lipidomics as an important key for the identification of beer-spoilage bacteria

Řezanka

Matoulková

Benada

et al. 2015

Lett Appl Microbiol

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“…24 The neutral loss scan of 141 from protonated or Li(I)-adducted plasmalogens cannot observe ethanolamine plasmalogens in complex lipid mixtures, due to its low abundances in the positive-ion mode. [1][2][3]23 Thus, the neutral loss scan of 141 could not be a useful tool to discover ethanolamine plasmalogens from complex biological samples, and a new diagnostic strategy is needed. To the best of our knowledge, this is the first application for Ag(I) adduction to be used for the identification of ethanolamine plasmalogens.…”

Section: -14mentioning

confidence: 99%

“…In mammals, the sn-1 position is typically derived from C16:0, C18:0 or C18:1 vinyl ether moieties. 1 Plasmalogens are commonly found in animal tissues, and they contain mostly ethanolamine or choline as their head groups. The vinyl ether double bond of plamalogens is targeted by various oxidants, such as peroxy radicals and metal ions.…”

Section: Introductionmentioning

confidence: 99%

Identification of Ethanolamine Plasmalogens from Complex Lipid Mixtures by MS/MS and Ag Adduction

Kim

Koh

et al. 2012

ANAL. SCI.

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A neutral loss scan of 141, corresponding to a phosphoethanolamine head group, has been commonly used for the determination of various glycerophosphoethanolamine species in complex lipid mixtures. However, the neutral loss of 141 Da is not a major fragmentation pathway in the collision-induced dissociation (CID) of ethanolamine plasmalogens in the positive-ion mode. Thus, the use of the neutral loss scan of 141 can be problematic to observe all possible ethanolamine phospholipids in biological samples. Ethanolamine plasmalogens could easily form adducts with Ag(I) ions, and the CID of Ag(I)-adducted ethanolamine plasmalogens provided abundant head group loss of 141 with higher collision energy. Thus, all ethanolamine plasmalogens could be identified from a neutral loss scan of 141 after Ag(I) adduction. This novel approach could be a useful diagnostic tool to observe most of the possible glycerophosphoethanolamine species in complex lipid mixtures.

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“…The reason for this is that lipid species and the applied internal standard (IS) of the given lipid class are co‐eluting or eluting within a narrow time window. Consequently, this method facilitates the identification of PLs and provides the most reliable quantification data because of the almost identical matrix effect during the ionization process (Cífková et al, ; Cífková, Hájek, Lísa, & Holčapek, ; Pati et al, ; Řezanka, Siristova, Matoulková, & Sigler, ).…”

Section: Introductionmentioning

confidence: 99%

Plasma phospholipid profiling of a mouse model of anxiety disorder by hydrophilic interaction liquid chromatography coupled to high‐resolution mass spectrometry

Berkecz

Körmöczi

Tömösi

et al. 2018

Biomedical Chromatography

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Glycerophospholipids (PLs), as amphipathic small molecules and the main constituents of biological membranes, play an important role in several cellular processes, even though their accurate identification from complex biological samples remains a challenge. In this paper, we report a fast and comprehensive HILIC-ESI-MS method for the analysis of glycerophospholipid classes using high-resolution mass spectrometry in negative mode. The final method enabled the quantitative analysis of 130 endogenous PL species in mouse plasma. The application of the method developed was to find differences of plasma PL composition in a mouse model of anxiety disorder. In the case of four PL classes and 35 PL species, significant differences were observed comparing low anxiety-related behavior with high anxiety-related behavior groups. The most characteristic trend was up-regulation in both the PL classes and PL species, and decreases were only detected in two phosphatidylcholines among 35 species in mice having elevated anxiety.

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Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Cited by 30 publications

References 48 publications

Lipidomics as an important key for the identification of beer-spoilage bacteria

Lipidomics as an important key for the identification of beer-spoilage bacteria

Identification of Ethanolamine Plasmalogens from Complex Lipid Mixtures by MS/MS and Ag Adduction

Plasma phospholipid profiling of a mouse model of anxiety disorder by hydrophilic interaction liquid chromatography coupled to high‐resolution mass spectrometry

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