Hydrophilic polymeric monoliths containing choline phosphate for separation science applications

Wang, Qiqin; Wu, Huihui; Peng, Kun; Jin, Hanying; Wang, Yuqiang; Crommen, Jacques; Jiang, Zhengjin

doi:10.1016/j.aca.2017.11.032

Cited by 30 publications

(12 citation statements)

References 39 publications

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“…Based on commercially available silica monoliths, a plethora of silica‐based monoliths were prepared and decorated by anchoring a layer of polar or ion‐exchange moieties of carboxylic acids, amides, even polyacrylamide. Polymeric HILIC monoliths can be easily fabricated via free radical polymerization as several hydrophilic monomers with various moieties are commercially available . Besides AuNPs used for surface modification of monolith in HILIC, cyclodextrin molecular tube, a new comer of cyclodextrin family with large and hydrophilic outer area and stable structure, was entrapped into a poly(HEMA‐ co ‐pentaerythritol triacrylate) monolith via host–guest self‐assembly.…”

Section: Application Of Monolithic Materials For Enrichment and Fractmentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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Section: Application Of Monolithic Materials For Enrichment and Fractmentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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“…The analyses of the triad peptides were done in Parallel Reaction Monitoring (PRM) mode. Specifically, the 13 In terms of data processing, chromatogram overlay and smoothing were performed using DataAnalysis 4.2 (Bruker, German). Then all raw data were exported as.…”

Section: Lc and Ms Conditions And Settingsmentioning

confidence: 99%

“…the MS analysis of the deamidation of Asn-containing residues in mAbs is due to the fact that there is only a single Dalton mass shift between the native form (Asn) and deamidated (n-Asp and isoAsp) forms (see Fig. S1), resulting in the overlap of mass peaks from the deamidated peptides and the 13 C isotopes of the corresponding native form peptides [6]. Hence, these native and modified peptides need to be chromatographically separated before MS analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a series of phospholipid-functionalized monoliths were developed for studying drug-membrane interactions [9][10][11] and predicting the phospholipidosis-inducing risk of drug candidates [12]. Moreover, these bio-membrane mimicking monoliths exhibited good separation ability for a wide range of compounds including small molecules, peptides, and intact proteins [9,11,13,14] owing to their mixed-mode interactions. Therefore, it is of interest to investigate the applicability of these phospholipidfunctionalized monoliths for the separation of deamidation related residues of mAbs.…”

Section: Introductionmentioning

confidence: 99%

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Separation of deamidated peptides with mixed-mode chromatography using phospholipid-functionalized monolithic stationary phases

Liu

Bults

Bischoff

et al. 2019

Journal of Chromatography A

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“…Indeed, phosphatidylcholine modified monolithic stationary phases were successfully used as immobilized artificial membranes . After chain‐end n ‐butyl group is introduced in zwitterionic functional group, higher selectivity and efficiency is achieved when compared to classical phoshorylcholine methacrylate with ethyl end group .…”

Section: Introductionmentioning

confidence: 99%

Controlling selectivity of polymer-based monolithic stationary phases

2019

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In this work, we aimed to prepare a monolithic capillary column that allowed an isocratic separation of ten dopamine precursors and metabolites in a single run. Segments of five zwitterion sulfobetaine polymer monoliths have been modified by zwitterion phoshorylcholine by using an ultraviolet‐initiated two‐step photografting. Columns with 0, 33, 50, 66, and 100% of modified length were prepared. Effect of length of the modified segment and mobile phase composition has been tested. All columns provided dual‐retention mechanism with reversed‐phase retention in highly aqueous mobile phase and hydrophilic interaction mechanism in highly organic mobile phase. The retention mechanism was controlled by the composition of the mobile phase and has been described by a three‐parameter model. We have used regression parameters to characterize the retention of analyzed compounds and to study individual pathways of dopamine metabolism. Comprehensive optimization of mobile phase composition allowed to find an optimal composition of the mobile phase and stationary phase surface chemistry arrangement to achieve desired separation. Optimized columns provided an isocratic separation of all tested compounds in less than nine min.

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Hydrophilic polymeric monoliths containing choline phosphate for separation science applications

Cited by 30 publications

References 39 publications

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Separation of deamidated peptides with mixed-mode chromatography using phospholipid-functionalized monolithic stationary phases

Controlling selectivity of polymer-based monolithic stationary phases

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