Hydrophilically stabilized gold nanostars as SERS labels for tissue imaging of the tumor suppressor p63 by immuno-SERS microscopy

Schütz, Max; Steinigeweg, Dennis; Salehi, Mohammad; Kömpe, Karsten; Schlücker, Sebastian

doi:10.1039/c0cc05229a

Cited by 156 publications

(125 citation statements)

References 19 publications

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“…Immuno-labeling SERS has was demonstrated for detecting antigens in tissues [334], and was continued using Au nanostar SERS labels for imaging a tumor suppressor in prostate biopsies [335]. Other ex vivo studies of human tissue include breast adenocarcinoma diagnosis using Au/SiO2 nanoclouds [336], colorectal cancer detection by SERS-based blood serum screening [337], measurement of normal and cancerous nasopharyngeal tissue [338], and colon carcinoma vs normal tissue [339].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

268

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

268

189

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“…A stoichiometric ratio of Ra 1 : Ra 2 ¼ 100 : 1 was chosen to ensure the steric accessibility of the carboxyl groups in the densely packed SAM. 19,20,39 The addition of DTNB-MEG-OH (Ra 1 ) and DTNB-TEG-CO 2 H (Ra 2 ) to the metal colloid leads to a hydrophilically stabilized self-assembled monolayer comprising two different terminal spacer units (dual spacer-SAM, cf. Fig.…”

Section: Characterization Of Antibody-sers Label Conjugatesmentioning

confidence: 99%

“…1B and Scheme 1A). [17][18][19][20] The benets of using MEG-OH and TEG-CO 2 H as hydrophilic spacers attached to aromatic Raman labels include the increased stability of the SERS labels due to the EG termini and the controllable bioconjugation via the MEG-OH/TEG-CO 2 H stoichiometry, together with the advantages of a SAM (maximum surface coverage with Raman reporters, uniform molecular orientation, and minimal coadsorption of other molecules from the surrounding onto the metal surface). SERS immunoassays have been reported for the detection of cell surface antigens, 21 immunoglobulin G (IgG), 8,13 protein A,…”

Section: Introductionmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

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Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono-and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL À1 . The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size.

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“…Antibodies directed against the protein p63 were conjugated to SERS labels with this protocol and were employed in immunohistochemistry. 55,94,121 b Polymer-encapsulated SERS nanoprobes. Polymerencapsulated SERS labels oen provide terminal functional groups from the polymer shell.…”

Section: Conjugation Of Ligands To Protected Sers Labelsmentioning

confidence: 99%

“…54 To avoid the use of CTAB and toxic DMF, Schütz et al have developed a biocompatible synthesis route towards monodisperse gold nanostars ( Fig. 4F) in water, with sodium citrate and hydroquinone as reducing/capping agents; 55 it was demonstrated that the as-prepared gold nanostars can be used as efficient SERS labels for tissue imaging.…”

mentioning

confidence: 99%

Rational design and synthesis of SERS labels

Wang

Schlücker

2013

Analyst

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143

147

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SERS labels are a new class of nanotags for optical detection based on Raman scattering. Central advantages include their spectral multiplexing capacity due to the small line width of vibrational Raman bands, quantification based on spectral intensities, high photostability, minimization of autofluorescence from biological specimens via red to near-infrared (NIR) excitation, and the need for only a single laser excitation line. Current concepts for the rational design and synthesis of SERS labels are summarized in this review. Chemical constituents of SERS labels are the plasmonically active metal colloids for signal enhancement upon resonant laser excitation, organic Raman reporter molecules for adsorption onto the metal surface for identification, and an optional protective shell. Different chemical approaches towards the synthesis of rationally designed SERS labels are highlighted, including also their subsequent bioconjugation.

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Hydrophilically stabilized gold nanostars as SERS labels for tissue imaging of the tumor suppressor p63 by immuno-SERS microscopy

Cited by 156 publications

References 19 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Rational design and synthesis of SERS labels

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