Hydrophobic affinity partition in aqueous two-phase systems of erythrocytes from different species

Walter, Harry; Krob, Eugene J.; Tung, Rita

doi:10.1016/0014-4827(76)90294-9

Cited by 35 publications

(11 citation statements)

References 28 publications

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“…In the present investigations, surface structures of group A streptococci responsible for hydrophobicity were further characterized. Partition in aqueous polyethylene glycol (PEG)dextran two-phase systems containing hydrophobic groups covalently bound to PEG was used to study the hydrophobic surface properties of the bacterial cells (1,2,11,24,40,41,43). LTA extracted from group A streptococci showed a hydrophobic interaction liability similar to that of whole organisms.…”

mentioning

confidence: 99%

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Miörner¹,

Johansson²,

Kronvall³

1983

Infect Immun

106

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MATERIALS AND METHODS Bacterial strains. A total of 56 bacterial strains were included in the study. The bacterial species and number of strains were as follows: group A streptococci, 34; group C streptococci, 5; group G streptococci, 6; Klebsiella pneumoniae, 3; Pseudomonas aeruginosa, 4; and Escherichia coli, 4. The strains were kindly provided by J. Rotta, Prague, Czechoslovakia (28 strains), and L. W. Wannamaker, Minneapolis, Minn.(2 strains), or were obtained consecutively from clinical specimens sent to the Clinical Microbiology Laboratory, University Hospital, Lund, Sweden. Strains were stored at -90°C in Todd-Hewitt broth supplemented with fetal calf serum and on blood agar plates at 4°C. Bacterial strains were inoculated in Todd-Hewitt broth and incubated for 15 to 17.5 h at 37°C. Bacteria were harvested by centrifugation at 3,500 rpm for 10 min and washed twice in phosphate-buffered 336 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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mentioning

confidence: 99%

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Miörner¹,

Johansson²,

Kronvall³

1983

Infect Immun

106

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“…The PEG backbone of P-PEG assures that the P-PEG will be in the top (or PEG-rich) phase and thus 'pull' membranes (or cells) 'up' in accordance 292 with the degree of hydrophobic interaction between the exposed membrane surface and the palmytoyl residue. That membrane charge is not involved in determining the partition of cells in this phase system (containing P-PEG and NaCl) is also shown by the fact that complete removal of human red cell sialic acid by neuraminidase treatment does not diminish the partition coefficient of erythrocytes [20].…”

Section: Resultsmentioning

confidence: 88%

Hydrophobic affinity partition in aqueous two‐phase systems containing polyethylene glycol)‐palmitate of rightside‐out and inside‐out vesicles from human erythrocyte membranes

Walter¹,

Krob²

1976

FEBS Letters

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“…We have shown in unpublished studies that 76-93% of fresh phagocytes lose their capacity to adhere and spread normally on glass and plastic surfaces after separation through ficoll or Percoll, a relatively new medium for density gradient purification of phagocytes consisting of polyvinylpyrrolidonecoated silic particles and not reportedly affecting cell function [19]. Given the sensitivity of polymer phase partitioning to minor cell surface property changes [4,6,7,11], we chose not to attempt cell purification procedures other than gravity or dextran sedimentation for these studies. The fresh buffy coat suspensions used, therefore, contained significant numbers of contaminating nonphagocytic cells, principally lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Surface charge and hydrophobic properties of fresh and cryopreserved blood phagocytes as determined by partition in two‐phase aqueous polymer systems

et al. 1986

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Cell partitioning in two-phase aqueous polymer systems was used to examine hydrophobic and surface charge-related membrane properties of fresh and cryopreserved human blood phagocytes. This technique is highly sensitive to cell surface characteristics, and the partition behavior depends exponentially on the membrane properties involved. The transition from fresh to cryopreserved and reconstituted cells was accompanied by a significant loss of net negative charge without detectable alteration in hydrophobic membrane properties as detected by the partition technique. The partition coefficient (PC), which is the proportion of cells partitioning into the upper phase, when measured for fresh cells mixed with dimethyl sulfoxide (Me2SO) and cryopreserved leukocytes. No difference was detected between the PCs of the total leukocytes and phagocytes as determined by differential leukocyte counts of the upper polymer phase. The significantly reduced PC of cells prepared by dextran (Dx) separation in both charge-sensitive and -insensitive systems is attributable to the capacity of Dx to adsorb, in part irreversibly, to cells so that those carrying Dx tend to partition with Dx in the lower phase. These results serve to illustrate the utility of partitioning as a highly sensitive method to probe leukocyte surface membrane properties.

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Hydrophobic affinity partition in aqueous two-phase systems of erythrocytes from different species

Cited by 35 publications

References 28 publications

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Hydrophobic affinity partition in aqueous two‐phase systems containing polyethylene glycol)‐palmitate of rightside‐out and inside‐out vesicles from human erythrocyte membranes

Surface charge and hydrophobic properties of fresh and cryopreserved blood phagocytes as determined by partition in two‐phase aqueous polymer systems

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