Hydrophobic characterization of intracellular lipids in situ by Nile Red red/yellow emission ratio

Diaz, Giacomo; Melis, Marta; Batetta, Barbara; Angius, Fabrizio; Falchi, Angela Maria

doi:10.1016/j.micron.2008.01.001

Cited by 151 publications

(123 citation statements)

References 15 publications

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“…To test the hypothesis, physiological experiments were performed by using the lipid dye Nile red. Nile red has been reported to be used as a general lipid dye for both polar and nonpolar lipid in different excitation ranges (45). As shown in Fig.…”

Section: Rpl4 Mutation Resulted In Reduced Lipid Accumulation In Merimentioning

confidence: 99%

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Sun

Hicks

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The vacuole is the most prominent compartment in plant cells and is important for ion and protein storage. In our effort to search for key regulators in the plant vacuole sorting pathway, ribosomal large subunit 4 (rpl4d) was identified as a translational mutant defective in both vacuole trafficking and normal development. Polysome profiling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no significant change in the general mRNA distribution pattern. Ribsomal profiling data indicated that genes in the lipid metabolism pathways were translationally down-regulated in the rpl4d mutant. Live imaging studies by Nile red staining suggested that both polar and nonpolar lipid accumulation was reduced in meristem tissues of rpl4d mutants. Pharmacological evidence showed that sterol and sphingolipid biosynthetic inhibitors can phenocopy the defects of the rpl4d mutant, including an altered vacuole trafficking pattern. Genetic evidence from lipid biosynthetic mutants indicates that alteration in the metabolism of either sterol or sphingolipid biosynthesis resulted in vacuole trafficking defects, similar to the rpl4d mutant. Tissue-specific complementation with key enzymes from lipid biosynthesis pathways can partially rescue both vacuole trafficking and auxin-related developmental defects in the rpl4d mutant. These results indicate that lipid metabolism modulates auxin-mediated tissue differentiation and endomembrane trafficking pathways downstream of ribosomal protein function.

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Section: Rpl4 Mutation Resulted In Reduced Lipid Accumulation In Merimentioning

confidence: 99%

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Sun

Hicks

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Nile red stains positively for lipids in plants Pighin et al, 2004;Schmidt and Herman, 2008;Dietrich et al, 2009), fluorescing red in the presence of polar lipids and yellow when staining esterified cholesterol and triacylglycerols (Diaz et al, 2008). This approach showed polar lipids in the PBS of maize, but in the oat parental line, only the MS and some of the veinal cells stained (Fig.…”

Section: Maize Chromosome 3 Introduction Causes Lipid Deposition In Omentioning

confidence: 91%

Individual Maize Chromosomes in the C3 Plant Oat Can Increase Bundle Sheath Cell Size and Vein Density

et al. 2012

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C4 photosynthesis has evolved in at least 66 lineages within the angiosperms and involves alterations to the biochemistry, cell biology, and development of leaves. The characteristic “Kranz” anatomy of most C4 leaves was discovered in the 1890s, but the genetic basis of these traits remains poorly defined. Oat × maize addition lines allow the effects of individual maize (Zea mays; C4) chromosomes to be investigated in an oat (Avena sativa; C3) genetic background. Here, we have determined the extent to which maize chromosomes can introduce C4 characteristics into oat and have associated any C4-like changes with specific maize chromosomes. While there is no indication of a simultaneous change to C4 biochemistry, leaf anatomy, and ultrastructure in any of the oat × maize addition lines, the C3 oat leaf can be modified at multiple levels. Maize genes encoding phosphoenolpyruvate carboxylase, pyruvate, orthophosphate dikinase, and the 2′-oxoglutarate/malate transporter are expressed in oat and generate transcripts of the correct size. Three maize chromosomes independently cause increases in vein density, and maize chromosome 3 results in larger bundle sheath cells with increased cell wall lipid deposition in oat leaves. These data provide proof of principle that aspects of C4 biology could be integrated into leaves of C3 crops.

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“…Nile Red is a selective lipid-specific dye that is strongly fluorescent only in a hydrophobic environment. Its emission spectrum shifts depending on the polarity of its microenvironment, fluorescing magenta in the presence of polar lipids and green in the presence of neutral lipids (Diaz et al, 2008). Confocal laser scanning microscopy of C. forskohlii root cork stained with Nile Red indicated that the observed structures were oil bodies and that the composition of the lipophilic content was heterogeneous.…”

Section: Identification Of Unique Lipophilic Organelles In C Forskohmentioning

confidence: 99%

Manoyl Oxide (13R), the Biosynthetic Precursor of Forskolin, Is Synthesized in Specialized Root Cork Cells in Coleus forskohlii

et al. 2014

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Hydrophobic characterization of intracellular lipids in situ by Nile Red red/yellow emission ratio

Cited by 151 publications

References 15 publications

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Individual Maize Chromosomes in the C3 Plant Oat Can Increase Bundle Sheath Cell Size and Vein Density

Manoyl Oxide (13R), the Biosynthetic Precursor of Forskolin, Is Synthesized in Specialized Root Cork Cells in Coleus forskohlii

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