Hydrophobic cryogels for DNA adsorption: Effect of embedding of monosize microbeads into cryogel network on their adsorptive performances

Çorman, M. Emin; Bereli, Nilay; Özkara, Serpil; Uzun, Lokman; Deni̇zli̇, Adil

doi:10.1002/bmc.2954

Cited by 32 publications

(16 citation statements)

References 39 publications

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“…The water uptake ratios of the membranes were determined by using deionized water [21].…”

Section: Characterization Of Membranesmentioning

confidence: 99%

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PolyUracil Membranes for Rapid and Effective RNA Purification

Armutçu¹

2020

Adıyaman University Journal of Science

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Ribonucleic acid (RNA) plays a critically important role in cellular defense, deoxyribonucleic acid (DNA) replication, transcription, and gene expression for living organisms as well as, a lot of diseases such as cancer, immunodeficiency, tumors has been associated with RNA's disruption. For this purpose, supermacropores membranes were designed to purify RNA using nucleotide-based ligand. In this study, polymerizable uracil monomer as uracil methacrylate (UraM) was synthesized, and 2-hydroxyethyl methacrylate (HEMA)-based membranes [poly(HEMA-UraM)] were prepared by bulk polymerization under partially frozen conditions by copolymerization of monomers, UraM, and HEMA. These membranes were characterized via swelling studies, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). To optimize separation conditions, effects of pH, initial RNA concentration, time, and temperature on RNA adsorption capacity were examined. Maximum adsorption of RNA on poly(HEMA-UraM) membrane was found to be 15.52 mg/g for 0.5 mg/mL RNA initial concentration at 25.0°C with an optimum pH of 7.0. After ten repetitive adsorption-desorption cycles, the RNA adsorption capacity decreased only 3.68%.

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“…The water uptake ratios of the membranes were determined by using deionized water [21].…”

Section: Characterization Of Membranesmentioning

confidence: 99%

“…Membranes are three-dimensional polymeric system of interconnected networks of macropores with a size of 10-100 [20,21]. The large pores and superior flow properties of membranes have allowed these materials to be used in different applications [22].…”

Section: Introductionmentioning

confidence: 99%

PolyUracil Membranes for Rapid and Effective RNA Purification

Armutçu¹

2020

Adıyaman University Journal of Science

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“…Maximum adsorbed pDNA was 32.4 mg/g. Çorman et al, prepared an affinity adsorbent cyrogel for the purification of DNA via hydrophobic interactions [32]. Experimental studies were carried on three types of PHEMA based cryogels embedded PHEMA/MATrp beads, PHEMA/MATrp and PHEMA.…”

Section: Dna Purification By Affinity Chromatographymentioning

confidence: 99%

DNA Purification by Solid Phase Extraction (SPE) Methods

Topçu¹,

Aşır²,

Türkmen³

2016

HJBC

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Ö Z E T D NA'nın saflaştırılması veya izole edilmesi moleküler biyoloji, tıbbi çalışmalar ve gen tedavi çalışmaları için önem taşır. Özellikle PCR çalışmaları için yüksek saflıkta DNA molekülüne ihtiyaç duyulmaktadır. Nükleik asitlerin saflaştırılmasında sırasıyla çözücü özütlemesi ve katı faz özütlemesi olmak üzere iki yöntem kulanılır. Çözücü ekstraksiyonu kolay uygulanabilir olmasına rağmen; toksisite, yüksek oranda çözücü ihtiyacı, yöntemin uzun zaman alması ve düşük özgüllüğü gibi birtakım kısıtlamalar mecvuttur. Günümüzde çeşitli örneklerden DNA'nın saflaştırılması için katı faz özütlemesi (SPE) yaygın olarak kullanılmaktadır. Bu çalışmada; özellikle DNA'nın saflaştırılması için SPE yönteminin uygulamaları üzerinde durulmuş ve bilimsel çalışmalar özetlenmiştir. Anahtar KelimelerDNA, DNA ayrılması, DNA saflaştırılması, SPE. A B S T R A C T DNA isolation/purification is a widely used procedure for molecular biology, medical studies, gene therapy and especially PCR studies which needs high quality or extremely pure nucleic acid samples. Nucleic acid separation was mainly achieved by two methods; solvent extraction and solid phase extraction (SPE) methods. Although solvent extraction method is the simplest method, there are some drawbacks such as toxicity, more solvent requirement, time-consuming procedure and low specificity. Nowadays, there is a wide use of solid phase matrices to extract DNA from a variety of samples. In this review article; we basically focused on SPE for DNA purification methods.

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“…Cryogels based on organic and inorganic materials are new chemical adsorbents used in water treatment due to their excellent structural properties and flow dynamics . Their mechanical properties and chemical stability are improved although they have a macroporous structure –. Water soluble polymerizable monomers have been widely used to prepare cryogels for water treatment –.…”

Section: Introductionmentioning

confidence: 99%

Removal of organic water pollutant using magnetite nanomaterials embedded with ionic copolymers of 2‐acrylamido‐2‐methylpropane sodium sulfonate cryogels

Al‐Hussain

Ezzat

Gaffer

et al. 2017

Polymer International

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Magnetite cryogel composites as macroporous crosslinked matrices have received wide attention and attract much interest in the water purification and desalination industry. They can be used to produce effective adsorbents with high adsorption rate, capacity and desorption for water pollutants. In this work, the incorporation of magnetite nanoparticles into cryogels by the in situ method is proposed to increase the dispersion of nanoparticles in the gel composites and to produce effective magnetic materials with high adsorption capacities. Ionic sodium‐2‐acrylamido‐2‐methylpropane sulfonate (Na‐AMPS) monomer was selected to prepare cryogels as the homopolymer or copolymers with 2‐hydroxyethyl methacrylate (HEMA) or N‐vinyl pyrrolidone (VP) by the crosslinking polymerization technique in the frozen state. Magnetite nanoparticles were introduced into the cryogel by the in situ co‐precipitation method after introducing iron cations into the cryogel networks. The surface morphologies, crystal structure, magnetite content, thermal stability and magnetic properties were determined for the cryogels and their magnetite composites. The magnetite cryogel composites show significantly enhanced methylene blue dye removal in short times with higher adsorption efficiencies and good regeneration to form an effective adsorbent for water treatment. © 2017 Society of Chemical Industry

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Hydrophobic cryogels for DNA adsorption: Effect of embedding of monosize microbeads into cryogel network on their adsorptive performances

Cited by 32 publications

References 39 publications

PolyUracil Membranes for Rapid and Effective RNA Purification

PolyUracil Membranes for Rapid and Effective RNA Purification

DNA Purification by Solid Phase Extraction (SPE) Methods

Removal of organic water pollutant using magnetite nanomaterials embedded with ionic copolymers of 2‐acrylamido‐2‐methylpropane sodium sulfonate cryogels

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